File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Cellular level robotic surgery: Nanodissection of intermediate filaments in live keratinocytes

TitleCellular level robotic surgery: Nanodissection of intermediate filaments in live keratinocytes
Authors
KeywordsIntermediate filament
Desmosome
Atomic Force Microscopy
Cell-cell adhesion
Nanosurgery
Mechanical property
Issue Date2015
Citation
Nanomedicine: Nanotechnology, Biology, and Medicine, 2015, v. 11, n. 1, p. 137-145 How to Cite?
Abstract© 2015 Elsevier Inc. We present the nanosurgery on the cytoskeleton of live cells using AFM based nanorobotics to achieve adhesiolysis and mimic the effect of pathophysiological modulation of intercellular adhesion. Nanosurgery successfully severs the intermediate filament bundles and disrupts cell-cell adhesion similar to the desmosomal protein disassembly in autoimmune disease, or the cationic modulation of desmosome formation. Our nanomechanical analysis revealed that adhesion loss results in a decrease in cellular stiffness in both cases of biochemical modulation of the desmosome junctions and mechanical disruption of intercellular adhesion, supporting the notion that intercellular adhesion through intermediate filaments anchors the cell structure as focal adhesion does and that intermediate filaments are integral components in cell mechanical integrity. The surgical process could potentially help reveal the mechanism of autoimmune pathology-induced cell-cell adhesion loss as well as its related pathways that lead to cell apoptosis. From the Clinical Editor: This team of authors performed nanosurgery on the cytoskeleton of live cells using AFM based nanorobotics to achieve adhesiolysis, and mimic the effect of pathophysiological modulation of intercellular adhesions. This method could potentially help reveal the mechanism of autoimmune pathology-induced cell-cell adhesion loss as well as its related pathways that lead to cell apoptosis.
Persistent Identifierhttp://hdl.handle.net/10722/213449
ISSN
2015 Impact Factor: 5.671
2015 SCImago Journal Rankings: 1.886

 

DC FieldValueLanguage
dc.contributor.authorYang, Ruiguo-
dc.contributor.authorSong, Bo-
dc.contributor.authorSun, Zhiyong-
dc.contributor.authorLai, King Wai Chiu-
dc.contributor.authorFung, Carmen Kar Man-
dc.contributor.authorPatterson, Kevin C.-
dc.contributor.authorSeiffert-Sinha, Kristina-
dc.contributor.authorSinha, Animesh A.-
dc.contributor.authorXi, Ning-
dc.date.accessioned2015-07-28T04:07:19Z-
dc.date.available2015-07-28T04:07:19Z-
dc.date.issued2015-
dc.identifier.citationNanomedicine: Nanotechnology, Biology, and Medicine, 2015, v. 11, n. 1, p. 137-145-
dc.identifier.issn1549-9634-
dc.identifier.urihttp://hdl.handle.net/10722/213449-
dc.description.abstract© 2015 Elsevier Inc. We present the nanosurgery on the cytoskeleton of live cells using AFM based nanorobotics to achieve adhesiolysis and mimic the effect of pathophysiological modulation of intercellular adhesion. Nanosurgery successfully severs the intermediate filament bundles and disrupts cell-cell adhesion similar to the desmosomal protein disassembly in autoimmune disease, or the cationic modulation of desmosome formation. Our nanomechanical analysis revealed that adhesion loss results in a decrease in cellular stiffness in both cases of biochemical modulation of the desmosome junctions and mechanical disruption of intercellular adhesion, supporting the notion that intercellular adhesion through intermediate filaments anchors the cell structure as focal adhesion does and that intermediate filaments are integral components in cell mechanical integrity. The surgical process could potentially help reveal the mechanism of autoimmune pathology-induced cell-cell adhesion loss as well as its related pathways that lead to cell apoptosis. From the Clinical Editor: This team of authors performed nanosurgery on the cytoskeleton of live cells using AFM based nanorobotics to achieve adhesiolysis, and mimic the effect of pathophysiological modulation of intercellular adhesions. This method could potentially help reveal the mechanism of autoimmune pathology-induced cell-cell adhesion loss as well as its related pathways that lead to cell apoptosis.-
dc.languageeng-
dc.relation.ispartofNanomedicine: Nanotechnology, Biology, and Medicine-
dc.subjectIntermediate filament-
dc.subjectDesmosome-
dc.subjectAtomic Force Microscopy-
dc.subjectCell-cell adhesion-
dc.subjectNanosurgery-
dc.subjectMechanical property-
dc.titleCellular level robotic surgery: Nanodissection of intermediate filaments in live keratinocytes-
dc.typeArticle-
dc.description.natureLink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.nano.2014.08.008-
dc.identifier.scopuseid_2-s2.0-84919620748-
dc.identifier.volume11-
dc.identifier.issue1-
dc.identifier.spage137-
dc.identifier.epage145-
dc.identifier.eissn1549-9642-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats