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Article: Probing for chemotherapy-induced peripheral neuropathy in live dorsal root ganglion neurons with atomic force microscopy

TitleProbing for chemotherapy-induced peripheral neuropathy in live dorsal root ganglion neurons with atomic force microscopy
Authors
KeywordsDorsal root ganglion neurons
Chemotherapy-induced peripheral neuropathy
Cell mechanic
AFM live cell imaging
Nanoindentation
Issue Date2014
Citation
Nanomedicine: Nanotechnology, Biology, and Medicine, 2014, v. 10, n. 6, p. 1323-1333 How to Cite?
AbstractChemotherapy-induced peripheral neuropathy (CIPN) remains a major reason for cancer patients to withdraw from their lifesaving therapy. CIPN results in irreversible sensory and motor impairments; however, the epidemiology is largely unknown. Here, we report for the first time that chemotherapy drug vincristine not only reduced axonal regeneration in primary dorsal root ganglion neuron but also induced substantial changes in cell mechanical properties detected by atomic force microscopy (AFM). Confocal imaging analysis revealed vincristine-induced microtubule depolymerization. By using AFM for high-resolution live cell imaging and quantitative analysis, we observed significant changes in cell surface roughness and stiffness of vincristine-treated neurons. Elastic modulus was decreased (21-45%) with increasing dosage of vincristine. Further study with paclitaxel, another well-known CIPN drug, confirmed the link between cell mechanics and cytoskeleton organization. These data support that our system can be used for probing potential CIPN drugs that are of enormous benefit to new chemotherapy drug development. From the Clinical Editor: This study concludes that reduced cell elasticity in dorsal root ganglion neurons accompanies the development of chemotherapy-induced peripheral neuropathy, providing a model system that enables testing of upcoming chemotherapy agents for this particularly inconvenient and often treatment-limiting complication. © 2014 Elsevier Inc.
Persistent Identifierhttp://hdl.handle.net/10722/213421
ISSN
2015 Impact Factor: 5.671
2015 SCImago Journal Rankings: 1.886

 

DC FieldValueLanguage
dc.contributor.authorAu, Ngan Pan Bennett-
dc.contributor.authorFang, Yuqiang-
dc.contributor.authorXi, Ning-
dc.contributor.authorLai, King Wai Chiu-
dc.contributor.authorMa, Chi Him Eddie-
dc.date.accessioned2015-07-28T04:07:13Z-
dc.date.available2015-07-28T04:07:13Z-
dc.date.issued2014-
dc.identifier.citationNanomedicine: Nanotechnology, Biology, and Medicine, 2014, v. 10, n. 6, p. 1323-1333-
dc.identifier.issn1549-9634-
dc.identifier.urihttp://hdl.handle.net/10722/213421-
dc.description.abstractChemotherapy-induced peripheral neuropathy (CIPN) remains a major reason for cancer patients to withdraw from their lifesaving therapy. CIPN results in irreversible sensory and motor impairments; however, the epidemiology is largely unknown. Here, we report for the first time that chemotherapy drug vincristine not only reduced axonal regeneration in primary dorsal root ganglion neuron but also induced substantial changes in cell mechanical properties detected by atomic force microscopy (AFM). Confocal imaging analysis revealed vincristine-induced microtubule depolymerization. By using AFM for high-resolution live cell imaging and quantitative analysis, we observed significant changes in cell surface roughness and stiffness of vincristine-treated neurons. Elastic modulus was decreased (21-45%) with increasing dosage of vincristine. Further study with paclitaxel, another well-known CIPN drug, confirmed the link between cell mechanics and cytoskeleton organization. These data support that our system can be used for probing potential CIPN drugs that are of enormous benefit to new chemotherapy drug development. From the Clinical Editor: This study concludes that reduced cell elasticity in dorsal root ganglion neurons accompanies the development of chemotherapy-induced peripheral neuropathy, providing a model system that enables testing of upcoming chemotherapy agents for this particularly inconvenient and often treatment-limiting complication. © 2014 Elsevier Inc.-
dc.languageeng-
dc.relation.ispartofNanomedicine: Nanotechnology, Biology, and Medicine-
dc.subjectDorsal root ganglion neurons-
dc.subjectChemotherapy-induced peripheral neuropathy-
dc.subjectCell mechanic-
dc.subjectAFM live cell imaging-
dc.subjectNanoindentation-
dc.titleProbing for chemotherapy-induced peripheral neuropathy in live dorsal root ganglion neurons with atomic force microscopy-
dc.typeArticle-
dc.description.natureLink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.nano.2014.03.002-
dc.identifier.pmid24632247-
dc.identifier.scopuseid_2-s2.0-84905232719-
dc.identifier.volume10-
dc.identifier.issue6-
dc.identifier.spage1323-
dc.identifier.epage1333-
dc.identifier.eissn1549-9642-

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