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Article: Atomic force microscopy imaging of live mammalian cells

TitleAtomic force microscopy imaging of live mammalian cells
Authors
Keywordspolydimethylsiloxane
cell locomotion
cytoskeleton
atomic force microscopy
lamellipodia
Issue Date2013
Citation
Science China Life Sciences, 2013, v. 56, n. 9, p. 811-817 How to Cite?
AbstractAtomic force microscopy (AFM) was used to examine the morphology of live mammalian adherent and suspended cells. Time-lapse AFM was used to record the locomotion dynamics of MCF-7 and Neuro-2a cells. When a MCF-7 cell retracted, many small sawtooth-like filopodia formed and reorganized, and the thickness of cellular lamellipodium increased as the retraction progressed. In elongated Neuro-2a cells, the cytoskeleton reorganized from an irregular to a parallel, linear morphology. Suspended mammalian cells were immobilized by method combining polydimethylsiloxane-fabricated wells with poly-L-lysine electrostatic adsorption. In this way, the morphology of a single live lymphoma cell was imaged by AFM. The experimental results can improve our understanding of cell locomotion and may lead to improved immobilization strategies. © 2013 The Author(s).
Persistent Identifierhttp://hdl.handle.net/10722/213354
ISSN
2015 Impact Factor: 2.297
2015 SCImago Journal Rankings: 0.728

 

DC FieldValueLanguage
dc.contributor.authorLi, Mi-
dc.contributor.authorLiu, LianQing Q.-
dc.contributor.authorXi, Ning-
dc.contributor.authorWang, YueChao C.-
dc.contributor.authorDong, ZaiLi L.-
dc.contributor.authorXiao, XiuBin B.-
dc.contributor.authorZhang, WeiJing J.-
dc.date.accessioned2015-07-28T04:07:00Z-
dc.date.available2015-07-28T04:07:00Z-
dc.date.issued2013-
dc.identifier.citationScience China Life Sciences, 2013, v. 56, n. 9, p. 811-817-
dc.identifier.issn1674-7305-
dc.identifier.urihttp://hdl.handle.net/10722/213354-
dc.description.abstractAtomic force microscopy (AFM) was used to examine the morphology of live mammalian adherent and suspended cells. Time-lapse AFM was used to record the locomotion dynamics of MCF-7 and Neuro-2a cells. When a MCF-7 cell retracted, many small sawtooth-like filopodia formed and reorganized, and the thickness of cellular lamellipodium increased as the retraction progressed. In elongated Neuro-2a cells, the cytoskeleton reorganized from an irregular to a parallel, linear morphology. Suspended mammalian cells were immobilized by method combining polydimethylsiloxane-fabricated wells with poly-L-lysine electrostatic adsorption. In this way, the morphology of a single live lymphoma cell was imaged by AFM. The experimental results can improve our understanding of cell locomotion and may lead to improved immobilization strategies. © 2013 The Author(s).-
dc.languageeng-
dc.relation.ispartofScience China Life Sciences-
dc.subjectpolydimethylsiloxane-
dc.subjectcell locomotion-
dc.subjectcytoskeleton-
dc.subjectatomic force microscopy-
dc.subjectlamellipodia-
dc.titleAtomic force microscopy imaging of live mammalian cells-
dc.typeArticle-
dc.description.natureLink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s11427-013-4532-y-
dc.identifier.pmid23929002-
dc.identifier.scopuseid_2-s2.0-84883455481-
dc.identifier.volume56-
dc.identifier.issue9-
dc.identifier.spage811-
dc.identifier.epage817-

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