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Article: Imaging and measuring the biophysical properties of Fc gamma receptors on single macrophages using atomic force microscopy

TitleImaging and measuring the biophysical properties of Fc gamma receptors on single macrophages using atomic force microscopy
Authors
KeywordsImmunotherapy
Fc gamma receptor
Atomic force microscopy
Antibody
Macrophage
Issue Date2013
Citation
Biochemical and Biophysical Research Communications, 2013, v. 438, n. 4, p. 709-714 How to Cite?
AbstractFc gamma receptors (FcγR), widely expressed on effector cells (e.g., NK cells, macrophages), play an important role in clinical cancer immunotherapy. The binding of FcγRs to the Fc portions of antibodies that are attached to the target cells can activate the antibody-dependent cell-mediated cytotoxicity (ADCC) killing mechanism which leads to the lysis of target cells. In this work, we used atomic force microscopy (AFM) to observe the cellular ultra-structures and measure the biophysical properties (affinity and distribution) of FcγRs on single macrophages in aqueous environments. AFM imaging was used to obtain the topographies of macrophages, revealing the nanoscale cellular fine structures. For molecular interaction recognition, antibody molecules were attached onto AFM tips via a heterobifunctional polyethylene glycol (PEG) crosslinker. With AFM single-molecule force spectroscopy, the binding affinities of FcγRs were quantitatively measured on single macrophages. Adhesion force mapping method was used to localize the FcγRs, revealing the nanoscale distribution of FcγRs on local areas of macrophages. The experimental results can improve our understanding of FcγRs on macrophages; the established approach will facilitate further research on physiological activities involved in antibody-based immunotherapy. © 2013 Elsevier Inc.
Persistent Identifierhttp://hdl.handle.net/10722/213352
ISSN
2015 Impact Factor: 2.371
2015 SCImago Journal Rankings: 1.152

 

DC FieldValueLanguage
dc.contributor.authorLi, Mi-
dc.contributor.authorLiu, Lianqing-
dc.contributor.authorXi, Ning-
dc.contributor.authorWang, Yuechao-
dc.contributor.authorXiao, Xiubin-
dc.contributor.authorZhang, Weijing-
dc.date.accessioned2015-07-28T04:06:59Z-
dc.date.available2015-07-28T04:06:59Z-
dc.date.issued2013-
dc.identifier.citationBiochemical and Biophysical Research Communications, 2013, v. 438, n. 4, p. 709-714-
dc.identifier.issn0006-291X-
dc.identifier.urihttp://hdl.handle.net/10722/213352-
dc.description.abstractFc gamma receptors (FcγR), widely expressed on effector cells (e.g., NK cells, macrophages), play an important role in clinical cancer immunotherapy. The binding of FcγRs to the Fc portions of antibodies that are attached to the target cells can activate the antibody-dependent cell-mediated cytotoxicity (ADCC) killing mechanism which leads to the lysis of target cells. In this work, we used atomic force microscopy (AFM) to observe the cellular ultra-structures and measure the biophysical properties (affinity and distribution) of FcγRs on single macrophages in aqueous environments. AFM imaging was used to obtain the topographies of macrophages, revealing the nanoscale cellular fine structures. For molecular interaction recognition, antibody molecules were attached onto AFM tips via a heterobifunctional polyethylene glycol (PEG) crosslinker. With AFM single-molecule force spectroscopy, the binding affinities of FcγRs were quantitatively measured on single macrophages. Adhesion force mapping method was used to localize the FcγRs, revealing the nanoscale distribution of FcγRs on local areas of macrophages. The experimental results can improve our understanding of FcγRs on macrophages; the established approach will facilitate further research on physiological activities involved in antibody-based immunotherapy. © 2013 Elsevier Inc.-
dc.languageeng-
dc.relation.ispartofBiochemical and Biophysical Research Communications-
dc.subjectImmunotherapy-
dc.subjectFc gamma receptor-
dc.subjectAtomic force microscopy-
dc.subjectAntibody-
dc.subjectMacrophage-
dc.titleImaging and measuring the biophysical properties of Fc gamma receptors on single macrophages using atomic force microscopy-
dc.typeArticle-
dc.description.natureLink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.bbrc.2013.07.114-
dc.identifier.pmid23916706-
dc.identifier.scopuseid_2-s2.0-84883278270-
dc.identifier.volume438-
dc.identifier.issue4-
dc.identifier.spage709-
dc.identifier.epage714-
dc.identifier.eissn1090-2104-

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