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Article: Investigating the morphology and mechanical properties of blastomeres with atomic force microscopy

TitleInvestigating the morphology and mechanical properties of blastomeres with atomic force microscopy
Authors
KeywordsYoung's modulus
mechanical properties
embryo
blastomere
atomic force microscopy
Issue Date2013
Citation
Surface and Interface Analysis, 2013, v. 45, n. 8, p. 1193-1196 How to Cite?
AbstractAtomic force microscopy (AFM) was used to directly investigate the morphology and mechanical properties of blastomeres during the embryo development. With AFM imaging, the surface topography of blastomeres from two-cell, four-cell, and eight-cell stages was visualized, and the AFM images clearly revealed the blastomere's morphological changes during the different embryo developmental stages. The section measurements of the AFM topography images of the blastomeres showed that the axis of the embryos nearly kept constant during the two-cell, four-cell, and eight-cell stages. With AFM indenting, the mechanical properties of living blastomeres from several embryos were measured quantitatively under physiological conditions. The results of mechanical properties measurements indicated that the Young's modulus of the two blastomeres from two-cell embryo was different from each other, and the four blastomeres from the four-cell embryo also had variable Young's modulus. Besides, the blastomeres from two-cell embryos were significantly harder than blastomeres from four-cell embryos. These results can improve our understanding of the embryo development from the view of cell mechanics. Copyright © 2013 John Wiley & Sons, Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/213321
ISSN
2015 Impact Factor: 1.018
2015 SCImago Journal Rankings: 0.422

 

DC FieldValueLanguage
dc.contributor.authorLiu, Lianqing-
dc.contributor.authorLi, Mi-
dc.contributor.authorZhang, Changlin-
dc.contributor.authorWang, Liu-
dc.contributor.authorXi, Ning-
dc.contributor.authorWang, Yuechao-
dc.contributor.authorDong, Zaili-
dc.date.accessioned2015-07-28T04:06:53Z-
dc.date.available2015-07-28T04:06:53Z-
dc.date.issued2013-
dc.identifier.citationSurface and Interface Analysis, 2013, v. 45, n. 8, p. 1193-1196-
dc.identifier.issn0142-2421-
dc.identifier.urihttp://hdl.handle.net/10722/213321-
dc.description.abstractAtomic force microscopy (AFM) was used to directly investigate the morphology and mechanical properties of blastomeres during the embryo development. With AFM imaging, the surface topography of blastomeres from two-cell, four-cell, and eight-cell stages was visualized, and the AFM images clearly revealed the blastomere's morphological changes during the different embryo developmental stages. The section measurements of the AFM topography images of the blastomeres showed that the axis of the embryos nearly kept constant during the two-cell, four-cell, and eight-cell stages. With AFM indenting, the mechanical properties of living blastomeres from several embryos were measured quantitatively under physiological conditions. The results of mechanical properties measurements indicated that the Young's modulus of the two blastomeres from two-cell embryo was different from each other, and the four blastomeres from the four-cell embryo also had variable Young's modulus. Besides, the blastomeres from two-cell embryos were significantly harder than blastomeres from four-cell embryos. These results can improve our understanding of the embryo development from the view of cell mechanics. Copyright © 2013 John Wiley & Sons, Ltd.-
dc.languageeng-
dc.relation.ispartofSurface and Interface Analysis-
dc.subjectYoung's modulus-
dc.subjectmechanical properties-
dc.subjectembryo-
dc.subjectblastomere-
dc.subjectatomic force microscopy-
dc.titleInvestigating the morphology and mechanical properties of blastomeres with atomic force microscopy-
dc.typeArticle-
dc.description.natureLink_to_subscribed_fulltext-
dc.identifier.doi10.1002/sia.5254-
dc.identifier.scopuseid_2-s2.0-84880132806-
dc.identifier.volume45-
dc.identifier.issue8-
dc.identifier.spage1193-
dc.identifier.epage1196-
dc.identifier.eissn1096-9918-

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