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Article: Cellular biophysical dynamics and ion channel activities detected by AFM-based nanorobotic manipulator in insulinoma β-cells

TitleCellular biophysical dynamics and ion channel activities detected by AFM-based nanorobotic manipulator in insulinoma β-cells
Authors
KeywordsIon channel activities
Cellular stiffness
AFM nanorobotics
Insulinoma β-cells
Issue Date2013
Citation
Nanomedicine: Nanotechnology, Biology, and Medicine, 2013, v. 9, n. 5, p. 636-645 How to Cite?
AbstractDistinct biochemical, electrochemical and electromechanical coupling processes of pancreatic β-cells may well underlie different response patterns of insulin release from glucose and capsaicin stimulation. Intracellular Ca2+ levels increased rapidly and dose-dependently upon glucose stimulation, accompanied with about threefold rapid increases in cellular stiffness. Subsequently, cellular stiffness diminished rapidly and settled at a value about twofold of the baseline. Capsaicin caused a similar transient increase in intracellular Ca2+ changes. However, cellular stiffness increased gradually to about twofold until leveling off. The current study characterizes for the first time the biophysical properties underlying glucose-induced biphasic responses of insulin secretion, distinctive from the slow and single-phased stiffness response to capsaicin despite similar changes in intracellular Ca2+ levels. The integrated AFM nanorobotics and optical investigation enables the fine dissection of mechano-property from ion channel activities in response to specific and non-specific agonist stimulation, providing novel biomechanical markers for the insulin secretion process. From the Clinical Editor: This study characterizes the biophysical properties underlying glucose-induced biphasic responses of insulin secretion. Integrated AFM nanorobotics and optical investigations provided novel biomechanical markers for the insulin secretion process. © 2013 Elsevier Inc.
Persistent Identifierhttp://hdl.handle.net/10722/213317
ISSN
2015 Impact Factor: 5.671
2015 SCImago Journal Rankings: 1.886

 

DC FieldValueLanguage
dc.contributor.authorYang, Ruiguo-
dc.contributor.authorXi, Ning-
dc.contributor.authorLai, King Wai Chiu-
dc.contributor.authorPatterson, Kevin C.-
dc.contributor.authorChen, Hongzhi-
dc.contributor.authorSong, Bo-
dc.contributor.authorQu, Chengeng-
dc.contributor.authorZhong, Beihua-
dc.contributor.authorWang, Donna H.-
dc.date.accessioned2015-07-28T04:06:52Z-
dc.date.available2015-07-28T04:06:52Z-
dc.date.issued2013-
dc.identifier.citationNanomedicine: Nanotechnology, Biology, and Medicine, 2013, v. 9, n. 5, p. 636-645-
dc.identifier.issn1549-9634-
dc.identifier.urihttp://hdl.handle.net/10722/213317-
dc.description.abstractDistinct biochemical, electrochemical and electromechanical coupling processes of pancreatic β-cells may well underlie different response patterns of insulin release from glucose and capsaicin stimulation. Intracellular Ca2+ levels increased rapidly and dose-dependently upon glucose stimulation, accompanied with about threefold rapid increases in cellular stiffness. Subsequently, cellular stiffness diminished rapidly and settled at a value about twofold of the baseline. Capsaicin caused a similar transient increase in intracellular Ca2+ changes. However, cellular stiffness increased gradually to about twofold until leveling off. The current study characterizes for the first time the biophysical properties underlying glucose-induced biphasic responses of insulin secretion, distinctive from the slow and single-phased stiffness response to capsaicin despite similar changes in intracellular Ca2+ levels. The integrated AFM nanorobotics and optical investigation enables the fine dissection of mechano-property from ion channel activities in response to specific and non-specific agonist stimulation, providing novel biomechanical markers for the insulin secretion process. From the Clinical Editor: This study characterizes the biophysical properties underlying glucose-induced biphasic responses of insulin secretion. Integrated AFM nanorobotics and optical investigations provided novel biomechanical markers for the insulin secretion process. © 2013 Elsevier Inc.-
dc.languageeng-
dc.relation.ispartofNanomedicine: Nanotechnology, Biology, and Medicine-
dc.subjectIon channel activities-
dc.subjectCellular stiffness-
dc.subjectAFM nanorobotics-
dc.subjectInsulinoma β-cells-
dc.titleCellular biophysical dynamics and ion channel activities detected by AFM-based nanorobotic manipulator in insulinoma β-cells-
dc.typeArticle-
dc.description.natureLink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.nano.2012.10.011-
dc.identifier.pmid23178285-
dc.identifier.scopuseid_2-s2.0-84879464376-
dc.identifier.volume9-
dc.identifier.issue5-
dc.identifier.spage636-
dc.identifier.epage645-
dc.identifier.eissn1549-9642-

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