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Article: A fully automated system for measuring cellular mechanical properties

TitleA fully automated system for measuring cellular mechanical properties
Authors
KeywordsMechanical properties of cells
Local scan
Automation
Atomic force microscopy (AFM)
Issue Date2012
Citation
Journal of Laboratory Automation, 2012, v. 17, n. 6, p. 443-448 How to Cite?
AbstractAs a novel effective label-free biomarker, the mechanical properties of cells have become increasingly important. However, the current methods of mapping cellular mechanical properties are mostly carried out manually, resulting in measurements that are time-consuming with low efficiency. In this article, a fully automated system of measuring the mechanical properties of cells based on atomic force microscopy (AFM) is proposed. In this system, the cells are recognized using an imageprocessing method and the relative position of the cell, and the AFM tip is accurately calibrated by the local scan method, meaning that the mechanical properties of cells can be measured sequentially without performing the step of AFM imaging. In addition, with the implementation of the automation, the high-throughput measurement of cellular mechanical properties can be performed rapidly. The capability of our system is validated on Raji cells, and the results indicate that the measurement rate of our system is 26 times faster than that of the traditional manual method, providing the technology for high-throughput measurement of cellular mechanical properties. © 2012 Society for Laboratory Automation and Screening.
Persistent Identifierhttp://hdl.handle.net/10722/213301
ISSN
2015 Impact Factor: 1.297
2015 SCImago Journal Rankings: 0.422

 

DC FieldValueLanguage
dc.contributor.authorWang, Zhibo-
dc.contributor.authorLiu, Lianqing-
dc.contributor.authorWang, Yuechao-
dc.contributor.authorXi, Ning-
dc.contributor.authorDong, Zaili-
dc.contributor.authorLi, Mi-
dc.contributor.authorYuan, Shuai-
dc.date.accessioned2015-07-28T04:06:49Z-
dc.date.available2015-07-28T04:06:49Z-
dc.date.issued2012-
dc.identifier.citationJournal of Laboratory Automation, 2012, v. 17, n. 6, p. 443-448-
dc.identifier.issn2211-0682-
dc.identifier.urihttp://hdl.handle.net/10722/213301-
dc.description.abstractAs a novel effective label-free biomarker, the mechanical properties of cells have become increasingly important. However, the current methods of mapping cellular mechanical properties are mostly carried out manually, resulting in measurements that are time-consuming with low efficiency. In this article, a fully automated system of measuring the mechanical properties of cells based on atomic force microscopy (AFM) is proposed. In this system, the cells are recognized using an imageprocessing method and the relative position of the cell, and the AFM tip is accurately calibrated by the local scan method, meaning that the mechanical properties of cells can be measured sequentially without performing the step of AFM imaging. In addition, with the implementation of the automation, the high-throughput measurement of cellular mechanical properties can be performed rapidly. The capability of our system is validated on Raji cells, and the results indicate that the measurement rate of our system is 26 times faster than that of the traditional manual method, providing the technology for high-throughput measurement of cellular mechanical properties. © 2012 Society for Laboratory Automation and Screening.-
dc.languageeng-
dc.relation.ispartofJournal of Laboratory Automation-
dc.subjectMechanical properties of cells-
dc.subjectLocal scan-
dc.subjectAutomation-
dc.subjectAtomic force microscopy (AFM)-
dc.titleA fully automated system for measuring cellular mechanical properties-
dc.typeArticle-
dc.description.natureLink_to_subscribed_fulltext-
dc.identifier.doi10.1177/2211068212460236-
dc.identifier.pmid23015516-
dc.identifier.scopuseid_2-s2.0-84875806055-
dc.identifier.volume17-
dc.identifier.issue6-
dc.identifier.spage443-
dc.identifier.epage448-
dc.identifier.eissn2211-0690-

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