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Article: Drug-induced changes of topography and elasticity in living B lymphoma cells based on atomic force microscopy | (Chinese Source)

TitleDrug-induced changes of topography and elasticity in living B lymphoma cells based on atomic force microscopy | (Chinese Source)
Authors
KeywordsAtomic force microscopy
Young′s modulus
Lymphoma
Elasticity
Force curve
Issue Date2012
Citation
Wuli Huaxue Xuebao/ Acta Physico - Chimica Sinica, 2012, v. 28, n. 6, p. 1502-1508 How to Cite?
AbstractAtomic force microscopy (AFM) provides a means for characterizing the surface topography and biophysical properties of individual living cells under near-physiological conditions. However, owing to the lack of adequate cellular immobilization methods, AFM imaging of living, suspended mammalian cells is still a big challenge. In this paper, a method is presented for immobilizing individual living B lymphoma cells that combines mechanical trapping with pillar arrays and electrostatic adsorption with poly-L-lysine. In this way, the topography and elasticity changes of individual B lymphoma cells that were stimulated with different concentrations of Rituximab were observed and measured dynamically. When the cell is stimulated by 0.2 mg·mL -1 Rituximab for 2 h, the cell topography becomes more corrugated and Young′s modulus decreases from 196 to 183 kPa. When the cell is stimulated by 0.5 mg·mL -1 Rituximab for 2 h, the cell topography changes more significantly and some tubercles appear, and Young′s modulus decreases from 234 to 175 kPa. These results thus provide a unique insight into the effects of Rituximab on individual cells. © Editorial office of Acta Physico-Chimica Sinica.
Persistent Identifierhttp://hdl.handle.net/10722/213243
ISSN
2015 Impact Factor: 0.844
2015 SCImago Journal Rankings: 0.265

 

DC FieldValueLanguage
dc.contributor.authorLi, Mi-
dc.contributor.authorLiu, Lian Qing-
dc.contributor.authorXi, Ning-
dc.contributor.authorWang, Yue Chao-
dc.contributor.authorDong, Zai Li-
dc.contributor.authorXiao, Xiu Bin-
dc.contributor.authorZhang, Wei Jing-
dc.date.accessioned2015-07-28T04:06:39Z-
dc.date.available2015-07-28T04:06:39Z-
dc.date.issued2012-
dc.identifier.citationWuli Huaxue Xuebao/ Acta Physico - Chimica Sinica, 2012, v. 28, n. 6, p. 1502-1508-
dc.identifier.issn1000-6818-
dc.identifier.urihttp://hdl.handle.net/10722/213243-
dc.description.abstractAtomic force microscopy (AFM) provides a means for characterizing the surface topography and biophysical properties of individual living cells under near-physiological conditions. However, owing to the lack of adequate cellular immobilization methods, AFM imaging of living, suspended mammalian cells is still a big challenge. In this paper, a method is presented for immobilizing individual living B lymphoma cells that combines mechanical trapping with pillar arrays and electrostatic adsorption with poly-L-lysine. In this way, the topography and elasticity changes of individual B lymphoma cells that were stimulated with different concentrations of Rituximab were observed and measured dynamically. When the cell is stimulated by 0.2 mg·mL -1 Rituximab for 2 h, the cell topography becomes more corrugated and Young′s modulus decreases from 196 to 183 kPa. When the cell is stimulated by 0.5 mg·mL -1 Rituximab for 2 h, the cell topography changes more significantly and some tubercles appear, and Young′s modulus decreases from 234 to 175 kPa. These results thus provide a unique insight into the effects of Rituximab on individual cells. © Editorial office of Acta Physico-Chimica Sinica.-
dc.languageeng-
dc.relation.ispartofWuli Huaxue Xuebao/ Acta Physico - Chimica Sinica-
dc.subjectAtomic force microscopy-
dc.subjectYoung′s modulus-
dc.subjectLymphoma-
dc.subjectElasticity-
dc.subjectForce curve-
dc.titleDrug-induced changes of topography and elasticity in living B lymphoma cells based on atomic force microscopy | (Chinese Source)-
dc.typeArticle-
dc.description.natureLink_to_subscribed_fulltext-
dc.identifier.doi10.3866/PKU.WHXB201203201-
dc.identifier.scopuseid_2-s2.0-84861312869-
dc.identifier.volume28-
dc.identifier.issue6-
dc.identifier.spage1502-
dc.identifier.epage1508-

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