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Article: Detecting CD20-Rituximab specific interactions on lymphoma cells using atomic force microscopy

TitleDetecting CD20-Rituximab specific interactions on lymphoma cells using atomic force microscopy
Authors
KeywordsRituximab
CD20 antigen
single-molecule force spectroscopy
atomic force microscopy
Issue Date2010
Citation
Science China Life Sciences, 2010, v. 53, n. 10, p. 1189-1195 How to Cite?
AbstractElucidating the underlying mechanisms of cell physiology is currently an important research topic in life sciences. Atomic force microscopy methods can be used to investigate these molecular mechanisms. In this study, single-molecule force spectroscopy was used to explore the specific recognition between the CD20 antigen and anti-CD20 antibody Rituximab on B lymphoma cells under near-physiological conditions. The CD20-Rituximab specific binding force was measured through tip functionalization. Distribution of CD20 on the B lymphoma cells was visualized three-dimensionally. In addition, the relationship between the intramolecular force and the molecular extension of the CD20-Rituximab complex was analyzed under an external force. These results facilitate further investigation of the mechanism of Rituximab's anti-cancer effect. © 2010 Science China Press and Springer-Verlag Berlin Heidelberg.
Persistent Identifierhttp://hdl.handle.net/10722/213125
ISSN
2015 Impact Factor: 2.297
2015 SCImago Journal Rankings: 0.728

 

DC FieldValueLanguage
dc.contributor.authorLi, Mi-
dc.contributor.authorLiu, Lian Qing-
dc.contributor.authorXi, Ning-
dc.contributor.authorWang, Yue Chao-
dc.contributor.authorDong, ZaiLi-
dc.contributor.authorLi, Guang Yong-
dc.contributor.authorXiao, Xiu Bin-
dc.contributor.authorZhang, Wei Jing-
dc.date.accessioned2015-07-28T04:06:13Z-
dc.date.available2015-07-28T04:06:13Z-
dc.date.issued2010-
dc.identifier.citationScience China Life Sciences, 2010, v. 53, n. 10, p. 1189-1195-
dc.identifier.issn1674-7305-
dc.identifier.urihttp://hdl.handle.net/10722/213125-
dc.description.abstractElucidating the underlying mechanisms of cell physiology is currently an important research topic in life sciences. Atomic force microscopy methods can be used to investigate these molecular mechanisms. In this study, single-molecule force spectroscopy was used to explore the specific recognition between the CD20 antigen and anti-CD20 antibody Rituximab on B lymphoma cells under near-physiological conditions. The CD20-Rituximab specific binding force was measured through tip functionalization. Distribution of CD20 on the B lymphoma cells was visualized three-dimensionally. In addition, the relationship between the intramolecular force and the molecular extension of the CD20-Rituximab complex was analyzed under an external force. These results facilitate further investigation of the mechanism of Rituximab's anti-cancer effect. © 2010 Science China Press and Springer-Verlag Berlin Heidelberg.-
dc.languageeng-
dc.relation.ispartofScience China Life Sciences-
dc.subjectRituximab-
dc.subjectCD20 antigen-
dc.subjectsingle-molecule force spectroscopy-
dc.subjectatomic force microscopy-
dc.titleDetecting CD20-Rituximab specific interactions on lymphoma cells using atomic force microscopy-
dc.typeArticle-
dc.description.natureLink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s11427-010-4070-9-
dc.identifier.pmid20953941-
dc.identifier.scopuseid_2-s2.0-77958510986-
dc.identifier.volume53-
dc.identifier.issue10-
dc.identifier.spage1189-
dc.identifier.epage1195-

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