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Article: Uptake of Retrograde Tracers by Intact Optic Nerve Axons: A New Way to Label Retinal Ganglion Cells

TitleUptake of Retrograde Tracers by Intact Optic Nerve Axons: A New Way to Label Retinal Ganglion Cells
Authors
Issue Date2015
PublisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
Citation
PLOS ONE, 2015, v. 10 n. 6, article no. e0128718 How to Cite?
AbstractRetrograde labelling of retinal ganglion cells with optic nerve transection often leads to degeneration of ganglion cells in prolonged experiments. Here we report that an intact optic nerve could uptake retrograde tracers applied onto the surface of the nerve, leading to high efficiency labelling of ganglion cells in the retina with long-term survival of cells. This method labelled a similar number of ganglion cells (2289 +/- 174 at 2 days) as the retrograde labeling technique from the superior colliculus (2250 +/- 94) or optic nerve stump (2279 +/- 114) after transection. This finding provides an alternative way to label retinal ganglion cells without damaging the optic tract. This will facilitate anatomical studies in identifying the morphology and connectivity of retinal ganglion cells, allowing secondary or triple labelling manipulations for long-term investigations.
Persistent Identifierhttp://hdl.handle.net/10722/212536
ISSN
2015 Impact Factor: 3.057
2015 SCImago Journal Rankings: 1.395
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLiang, YX-
dc.contributor.authorYang, J-
dc.contributor.authorYuan, TF-
dc.contributor.authorSo, KF-
dc.date.accessioned2015-07-21T02:39:49Z-
dc.date.available2015-07-21T02:39:49Z-
dc.date.issued2015-
dc.identifier.citationPLOS ONE, 2015, v. 10 n. 6, article no. e0128718-
dc.identifier.issn1932-6203-
dc.identifier.urihttp://hdl.handle.net/10722/212536-
dc.description.abstractRetrograde labelling of retinal ganglion cells with optic nerve transection often leads to degeneration of ganglion cells in prolonged experiments. Here we report that an intact optic nerve could uptake retrograde tracers applied onto the surface of the nerve, leading to high efficiency labelling of ganglion cells in the retina with long-term survival of cells. This method labelled a similar number of ganglion cells (2289 +/- 174 at 2 days) as the retrograde labeling technique from the superior colliculus (2250 +/- 94) or optic nerve stump (2279 +/- 114) after transection. This finding provides an alternative way to label retinal ganglion cells without damaging the optic tract. This will facilitate anatomical studies in identifying the morphology and connectivity of retinal ganglion cells, allowing secondary or triple labelling manipulations for long-term investigations.-
dc.languageeng-
dc.publisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action-
dc.relation.ispartofPLOS ONE-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titleUptake of Retrograde Tracers by Intact Optic Nerve Axons: A New Way to Label Retinal Ganglion Cells-
dc.typeArticle-
dc.identifier.emailLiang, YX: yxliang@hku.hk-
dc.identifier.emailYang, J: jianyang@hkucc.hku.hk-
dc.identifier.emailYuan, TF: tfyuan@hku.hk-
dc.identifier.emailSo, KF: hrmaskf@hku.hk-
dc.identifier.authorityLiang, YX=rp00510-
dc.identifier.authoritySo, KF=rp00329-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1371/journal.pone.0128718-
dc.identifier.pmid26065419-
dc.identifier.pmcidPMC4465746-
dc.identifier.hkuros244317-
dc.identifier.volume10-
dc.identifier.issue6-
dc.identifier.spagearticle no. e0128718-
dc.identifier.epagearticle no. e0128718-
dc.identifier.isiWOS:000356100900028-
dc.publisher.placeUnited States-

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