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Article: Streptobacillus hongkongensis sp. nov., isolated from patients with quinsy and septic arthritis, and emended descriptions of the genus Streptobacillus and Streptobacillus moniliformis

TitleStreptobacillus hongkongensis sp. nov., isolated from patients with quinsy and septic arthritis, and emended descriptions of the genus Streptobacillus and Streptobacillus moniliformis
Authors
Issue Date2014
PublisherSociety for General Microbiology. The Journal's web site is located at http://ijs.sgmjournals.org
Citation
International Journal of Systematic and Evolutionary Microbiology, 2014, v. 64 n. pt. 9, p. 3034-3039 How to Cite?
AbstractTwo bacterial strains, HKU33T and HKU34, were isolated in Hong Kong from the pus aspirated from the right peritonsillar abscess of a patient with quinsy and the left elbow joint fluid of another patient with tophaceous gout and left elbow septic arthritis, respectively. The bacteria were Gramstain-negative, non-motile, non-spore-forming, non-haemolytic pleomorphic bacilli. They grew best on Columbia agar with 5% defibrinated sheep blood in an anaerobic environment or aerobic environment with 5% CO2. They also grew on chocolate agar but not on MacConkey agar. They were catalase-and cytochrome oxidase-negative. They showed a unique profile of enzyme activities distinguishable from their closely related species. Phylogenetic analysis of the complete 16S rRNA gene, and partial groEL, gyrB and recA gene sequences showed the two isolates formed a distinct branch within the family Leptotrichiaceae, being related most closely to Streptobacillus moniliformis. Hierarchical cluster analysis of mass spectra of whole-cell protein contents showed that strains HKU33T and HKU34 were closely related to each other, but were distinct from Streptobacillus moniliformis, Sneathia sanguinegens and ‘Leptotrichia amnionii’. The DNA G+C content of strain HKU33T was 26.0±2.1 mol% (mean±SD; n=3). DNA-DNA hybridization demonstrated ≤45.02% DNA relatedness between the two isolates and Streptobacillus moniliformis CCUG 13453T. A novel species, Streptobacillus hongkongensis sp. nov., is proposed to accommodate strains HKU33T and HKU34, with HKU33T (=JCM 18691T=NCTC 13659T=DSM 26322T) designated the type strain. Emended descriptions of the genus Streptobacillus and Streptobacillus moniliformis are also given.
Persistent Identifierhttp://hdl.handle.net/10722/211857
ISSN
2017 Impact Factor: 1.932
2015 SCImago Journal Rankings: 1.088
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWoo, PCY-
dc.contributor.authorWu, AK-
dc.contributor.authorTsang, CC-
dc.contributor.authorLeung, KW-
dc.contributor.authorNgan, AH-
dc.contributor.authorCurreem, SOT-
dc.contributor.authorLam, KW-
dc.contributor.authorChen, JHK-
dc.contributor.authorChan, JFW-
dc.contributor.authorLau, SKP-
dc.date.accessioned2015-07-21T02:13:48Z-
dc.date.available2015-07-21T02:13:48Z-
dc.date.issued2014-
dc.identifier.citationInternational Journal of Systematic and Evolutionary Microbiology, 2014, v. 64 n. pt. 9, p. 3034-3039-
dc.identifier.issn1466-5026-
dc.identifier.urihttp://hdl.handle.net/10722/211857-
dc.description.abstractTwo bacterial strains, HKU33T and HKU34, were isolated in Hong Kong from the pus aspirated from the right peritonsillar abscess of a patient with quinsy and the left elbow joint fluid of another patient with tophaceous gout and left elbow septic arthritis, respectively. The bacteria were Gramstain-negative, non-motile, non-spore-forming, non-haemolytic pleomorphic bacilli. They grew best on Columbia agar with 5% defibrinated sheep blood in an anaerobic environment or aerobic environment with 5% CO2. They also grew on chocolate agar but not on MacConkey agar. They were catalase-and cytochrome oxidase-negative. They showed a unique profile of enzyme activities distinguishable from their closely related species. Phylogenetic analysis of the complete 16S rRNA gene, and partial groEL, gyrB and recA gene sequences showed the two isolates formed a distinct branch within the family Leptotrichiaceae, being related most closely to Streptobacillus moniliformis. Hierarchical cluster analysis of mass spectra of whole-cell protein contents showed that strains HKU33T and HKU34 were closely related to each other, but were distinct from Streptobacillus moniliformis, Sneathia sanguinegens and ‘Leptotrichia amnionii’. The DNA G+C content of strain HKU33T was 26.0±2.1 mol% (mean±SD; n=3). DNA-DNA hybridization demonstrated ≤45.02% DNA relatedness between the two isolates and Streptobacillus moniliformis CCUG 13453T. A novel species, Streptobacillus hongkongensis sp. nov., is proposed to accommodate strains HKU33T and HKU34, with HKU33T (=JCM 18691T=NCTC 13659T=DSM 26322T) designated the type strain. Emended descriptions of the genus Streptobacillus and Streptobacillus moniliformis are also given.-
dc.languageeng-
dc.publisherSociety for General Microbiology. The Journal's web site is located at http://ijs.sgmjournals.org-
dc.relation.ispartofInternational Journal of Systematic and Evolutionary Microbiology-
dc.rightsInternational Journal of Systematic and Evolutionary Microbiology. Copyright © Society for General Microbiology.-
dc.rightsThis is an author manuscript that has been accepted for publication in [Journal Title], copyright Society for General Microbiology, but has not been copy-edited, formatted or proofed. Cite this article as appearing in [Journal Title]. This version of the manuscript may not be duplicated or reproduced, other than for personal use or within the rule of 'Fair Use of Copyrighted Materials' (section 17, Title 17, US Code), without permission from the copyright owner, Society for General Microbiology. The Society for General Microbiology disclaims any responsibility or liability for errors or omissions in this version of the manuscript or in any version derived from it by any other parties. The final copy-edited, published article, which is the version of record, can be found at [Journal URL], and is freely available without a subscription.-
dc.titleStreptobacillus hongkongensis sp. nov., isolated from patients with quinsy and septic arthritis, and emended descriptions of the genus Streptobacillus and Streptobacillus moniliformis-
dc.typeArticle-
dc.identifier.emailWoo, PCY: pcywoo@hkucc.hku.hk-
dc.identifier.emailChen, JHK: jonchk@hku.hk-
dc.identifier.emailChan, JFW: jfwchan@hku.hk-
dc.identifier.emailLau, SKP: skplau@hkucc.hku.hk-
dc.identifier.authorityWoo, PCY=rp00430-
dc.identifier.authorityChan, JFW=rp01736-
dc.identifier.authorityLau, SKP=rp00486-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1099/ijs.0.061242-0-
dc.identifier.pmid24912824-
dc.identifier.scopuseid_2-s2.0-84907046000-
dc.identifier.hkuros245663-
dc.identifier.volume64-
dc.identifier.issuept. 9-
dc.identifier.spage3034-
dc.identifier.epage3039-
dc.identifier.isiWOS:000344912300017-
dc.publisher.placeUnited Kingdom-

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