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Article: Andrographolide exerted its antimicrobial effects by upregulation of human β-defensin-2 induced through p38 MAPK and NF-κB pathway in human lung epithelial cells

TitleAndrographolide exerted its antimicrobial effects by upregulation of human β-defensin-2 induced through p38 MAPK and NF-κB pathway in human lung epithelial cells
Authors
Issue Date2012
PublisherN R C Research Press. The Journal's web site is located at http://pubs.nrc-cnrc.gc.ca/cgi-bin/rp/rp2_desc_e?cjpp
Citation
Canadian Journal of Physiology and Pharmacology, 2012, v. 90 n. 5, p. 647-653 How to Cite?
AbstractAndrographis paniculata (Burm. f) Nees is a traditional herbal medicine for the treatment of infection and inflammation in China. Andrographolide (andro) is one of the major components. Human β-defensin-2 (hBD-2) is an inducible antimicrobial peptide that plays an important role in innate immunity. The present study aimed to investigate the effect of andro on upregulation of hBD-2 and the key signaling pathways involved in andro-induced hBD-2 expression. Real-time reverse transcription - PCR and Western blot assays showed that andro (1.0-10 µmol/L) can upregulate the expression of hBD-2 in a dose-dependent manner. Further studies suggested that hBD-2 mRNA and protein expression in responsive to andro were attenuated by pretreatment with SB203580 (an inhibitor of p38 mitogen-activated protein kinase (p38 MAPK)), MG-132 (an inhibitor of nuclear factor κB (NF-κB)), and an NF-κB activator inhibitor, but not by an inhibitor of ERK (PD98059) or by an inhibitor of JNK(SP600125). Moreover, we found that a second p38 MAPK inhibitor (SB202190) significantly blocked andro-mediated hBD-2 induction in SPC-A-1 lung epithelial cells. Finally, the p-c-Jun transcription factor activity assay also showed that AP-1 activity was induced by andro compared with the untreated group. We conclude that andro may exert its antimicrobial effects by upregulating the expression of hBD-2 through the p38 MAPK and NF-κB pathway.
Persistent Identifierhttp://hdl.handle.net/10722/211385
ISSN
2015 Impact Factor: 1.704
2015 SCImago Journal Rankings: 0.683

 

DC FieldValueLanguage
dc.contributor.authorShao, ZJ-
dc.contributor.authorZheng, XW-
dc.contributor.authorTing, F-
dc.contributor.authorHuang, J-
dc.contributor.authorChen, J-
dc.contributor.authorWu, YY-
dc.contributor.authorZhou, LM-
dc.contributor.authorTu, WW-
dc.contributor.authorLi, H-
dc.date.accessioned2015-07-09T06:43:54Z-
dc.date.available2015-07-09T06:43:54Z-
dc.date.issued2012-
dc.identifier.citationCanadian Journal of Physiology and Pharmacology, 2012, v. 90 n. 5, p. 647-653-
dc.identifier.issn0008-4212-
dc.identifier.urihttp://hdl.handle.net/10722/211385-
dc.description.abstractAndrographis paniculata (Burm. f) Nees is a traditional herbal medicine for the treatment of infection and inflammation in China. Andrographolide (andro) is one of the major components. Human β-defensin-2 (hBD-2) is an inducible antimicrobial peptide that plays an important role in innate immunity. The present study aimed to investigate the effect of andro on upregulation of hBD-2 and the key signaling pathways involved in andro-induced hBD-2 expression. Real-time reverse transcription - PCR and Western blot assays showed that andro (1.0-10 µmol/L) can upregulate the expression of hBD-2 in a dose-dependent manner. Further studies suggested that hBD-2 mRNA and protein expression in responsive to andro were attenuated by pretreatment with SB203580 (an inhibitor of p38 mitogen-activated protein kinase (p38 MAPK)), MG-132 (an inhibitor of nuclear factor κB (NF-κB)), and an NF-κB activator inhibitor, but not by an inhibitor of ERK (PD98059) or by an inhibitor of JNK(SP600125). Moreover, we found that a second p38 MAPK inhibitor (SB202190) significantly blocked andro-mediated hBD-2 induction in SPC-A-1 lung epithelial cells. Finally, the p-c-Jun transcription factor activity assay also showed that AP-1 activity was induced by andro compared with the untreated group. We conclude that andro may exert its antimicrobial effects by upregulating the expression of hBD-2 through the p38 MAPK and NF-κB pathway.-
dc.languageeng-
dc.publisherN R C Research Press. The Journal's web site is located at http://pubs.nrc-cnrc.gc.ca/cgi-bin/rp/rp2_desc_e?cjpp-
dc.relation.ispartofCanadian Journal of Physiology and Pharmacology-
dc.rightsCanadian Journal of Physiology and Pharmacology. Copyright © N R C Research Press.-
dc.titleAndrographolide exerted its antimicrobial effects by upregulation of human β-defensin-2 induced through p38 MAPK and NF-κB pathway in human lung epithelial cells-
dc.typeArticle-
dc.identifier.emailTu, WW: wwtu@hku.hk-
dc.identifier.authorityTu, WW=rp00416-
dc.identifier.doi10.1139/y2012-050-
dc.identifier.pmid22537555-
dc.identifier.hkuros200723-
dc.identifier.hkuros225533-
dc.identifier.volume90-
dc.identifier.issue5-
dc.identifier.spage647-
dc.identifier.epage653-
dc.publisher.placeCanada-

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