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Article: Transcriptomic Responses Of Corpuscle Of Stannius Gland Of Japanese Eels (anguilla Japonica) To Changes In Water Salinity

TitleTranscriptomic Responses Of Corpuscle Of Stannius Gland Of Japanese Eels (anguilla Japonica) To Changes In Water Salinity
Authors
Issue Date2015
Citation
Scientific Reports, 2015, v. 5, p. 9836 How to Cite?
AbstractPhysiological studies of a unique endocrine gland in fish, named corpuscles of Stannius (CS), described a Ca2+-regulatory function for this gland mediated by stanniocalcin-1, a hypocalcemic polypeptide hormone. However, to date, the endocrine functions of the glands have not been completely elucidated. We hypothesized that other unidentified active principles in the glands are involved in the regulation of plasma ion (Na+, Ca2+) and/or blood pressure. In this study, transcriptome sequencing of CS glands was performed using Japanese eels (Anguilla japonica) adapted to freshwater (FW) or seawater (SW) to reveal the presence and differential expression of genes encoding proteins related to the ion-osmoregulatory and pressor functions. We acquired a total of 14.1 Mb and 12.1 Mb quality-trimmed reads from the CS glands collected from FW and SW adapted eels, respectively. The de novo assembly resulted in 9254 annotated genes. Among them, 475 genes were differentially expressed with 357 up- and 118 down-regulated in the SW group. Gene ontology analysis further demonstrated the presence of natriuresis and pressor related genes. In summary, ours is the first study using high-throughput sequencing to identify gene targets that could explain the physiological importance of the CS glands.
Persistent Identifierhttp://hdl.handle.net/10722/209939

 

DC FieldValueLanguage
dc.contributor.authorGu, Jen_US
dc.contributor.authorLi, JWen_US
dc.contributor.authorTse, WKFen_US
dc.contributor.authorChan, TFen_US
dc.contributor.authorLai, KPen_US
dc.contributor.authorWong, CKCen_US
dc.date.accessioned2015-05-18T03:32:45Z-
dc.date.available2015-05-18T03:32:45Z-
dc.date.issued2015en_US
dc.identifier.citationScientific Reports, 2015, v. 5, p. 9836en_US
dc.identifier.urihttp://hdl.handle.net/10722/209939-
dc.description.abstractPhysiological studies of a unique endocrine gland in fish, named corpuscles of Stannius (CS), described a Ca2+-regulatory function for this gland mediated by stanniocalcin-1, a hypocalcemic polypeptide hormone. However, to date, the endocrine functions of the glands have not been completely elucidated. We hypothesized that other unidentified active principles in the glands are involved in the regulation of plasma ion (Na+, Ca2+) and/or blood pressure. In this study, transcriptome sequencing of CS glands was performed using Japanese eels (Anguilla japonica) adapted to freshwater (FW) or seawater (SW) to reveal the presence and differential expression of genes encoding proteins related to the ion-osmoregulatory and pressor functions. We acquired a total of 14.1 Mb and 12.1 Mb quality-trimmed reads from the CS glands collected from FW and SW adapted eels, respectively. The de novo assembly resulted in 9254 annotated genes. Among them, 475 genes were differentially expressed with 357 up- and 118 down-regulated in the SW group. Gene ontology analysis further demonstrated the presence of natriuresis and pressor related genes. In summary, ours is the first study using high-throughput sequencing to identify gene targets that could explain the physiological importance of the CS glands.en_US
dc.languageengen_US
dc.relation.ispartofScientific Reportsen_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titleTranscriptomic Responses Of Corpuscle Of Stannius Gland Of Japanese Eels (anguilla Japonica) To Changes In Water Salinityen_US
dc.typeArticleen_US
dc.identifier.emailLai, KP: balllai@hku.hken_US
dc.identifier.authorityLai, KP=rp01753en_US
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1038/srep09836en_US
dc.identifier.hkuros243219en_US
dc.identifier.volume5en_US
dc.identifier.spage9836en_US
dc.identifier.epage9836en_US

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