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Article: Planar cell polarity breaks bilateral symmetry by controlling ciliary positioning

TitlePlanar cell polarity breaks bilateral symmetry by controlling ciliary positioning
Authors
KeywordsAlleles
Animals
Body Patterning/genetics/*physiology
Carrier Proteins/genetics/metabolism
Cell Polarity/genetics/*physiology
Cilia/*physiology
Embryo
Functional Laterality/genetics/physiology
Membrane Proteins/deficiency/genetics/metabolism
Mice
Nerve Tissue Proteins/deficiency/genetics/metabolism
Mammalian/*cytology/*embryology/metabolism/pathology
Issue Date2010
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/nature
Citation
Nature, 2010, v. 466 n. 7304, p. 378-382 How to Cite?
AbstractDefining the three body axes is a central event of vertebrate morphogenesis. Establishment of left-right (L-R) asymmetry in development follows the determination of dorsal-ventral and anterior-posterior (A-P) body axes, although the molecular mechanism underlying precise L-R symmetry breaking in reference to the other two axes is still poorly understood. Here, by removing both Vangl1 and Vangl2, the two mouse homologues of a Drosophila core planar cell polarity (PCP) gene Van Gogh (Vang), we reveal a previously unrecognized function of PCP in the initial breaking of lateral symmetry. The leftward nodal flow across the posterior notochord (PNC) has been identified as the earliest event in the de novo formation of L-R asymmetry. We show that PCP is essential in interpreting the A-P patterning information and linking it to L-R asymmetry. In the absence of Vangl1 and Vangl2, cilia are positioned randomly around the centre of the PNC cells and nodal flow is turbulent, which results in disrupted L-R asymmetry. PCP in mouse, unlike what has been implicated in other vertebrate species, is not required for ciliogenesis, cilium motility, Sonic hedgehog (Shh) signalling or apical docking of basal bodies in ciliated tracheal epithelial cells. Our data suggest that PCP acts earlier than the unidirectional nodal flow during bilateral symmetry breaking in vertebrates and provide insight into the functional mechanism of PCP in organizing the vertebrate tissues in development.
Persistent Identifierhttp://hdl.handle.net/10722/208448
ISSN
2015 Impact Factor: 38.138
2015 SCImago Journal Rankings: 21.936
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorSong, Hen_US
dc.contributor.authorHu, Jen_US
dc.contributor.authorChen, Wen_US
dc.contributor.authorElliott, Gen_US
dc.contributor.authorAndre, Pen_US
dc.contributor.authorGao, Ben_US
dc.contributor.authorYang, Yen_US
dc.date.accessioned2015-03-11T03:01:07Z-
dc.date.available2015-03-11T03:01:07Z-
dc.date.issued2010en_US
dc.identifier.citationNature, 2010, v. 466 n. 7304, p. 378-382en_US
dc.identifier.issn0028-0836en_US
dc.identifier.urihttp://hdl.handle.net/10722/208448-
dc.description.abstractDefining the three body axes is a central event of vertebrate morphogenesis. Establishment of left-right (L-R) asymmetry in development follows the determination of dorsal-ventral and anterior-posterior (A-P) body axes, although the molecular mechanism underlying precise L-R symmetry breaking in reference to the other two axes is still poorly understood. Here, by removing both Vangl1 and Vangl2, the two mouse homologues of a Drosophila core planar cell polarity (PCP) gene Van Gogh (Vang), we reveal a previously unrecognized function of PCP in the initial breaking of lateral symmetry. The leftward nodal flow across the posterior notochord (PNC) has been identified as the earliest event in the de novo formation of L-R asymmetry. We show that PCP is essential in interpreting the A-P patterning information and linking it to L-R asymmetry. In the absence of Vangl1 and Vangl2, cilia are positioned randomly around the centre of the PNC cells and nodal flow is turbulent, which results in disrupted L-R asymmetry. PCP in mouse, unlike what has been implicated in other vertebrate species, is not required for ciliogenesis, cilium motility, Sonic hedgehog (Shh) signalling or apical docking of basal bodies in ciliated tracheal epithelial cells. Our data suggest that PCP acts earlier than the unidirectional nodal flow during bilateral symmetry breaking in vertebrates and provide insight into the functional mechanism of PCP in organizing the vertebrate tissues in development.en_US
dc.languageengen_US
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/natureen_US
dc.relation.ispartofNatureen_US
dc.subjectAllelesen_US
dc.subjectAnimalsen_US
dc.subjectBody Patterning/genetics/*physiologyen_US
dc.subjectCarrier Proteins/genetics/metabolismen_US
dc.subjectCell Polarity/genetics/*physiologyen_US
dc.subjectCilia/*physiologyen_US
dc.subjectEmbryoen_US
dc.subjectFunctional Laterality/genetics/physiologyen_US
dc.subjectMembrane Proteins/deficiency/genetics/metabolismen_US
dc.subjectMiceen_US
dc.subjectNerve Tissue Proteins/deficiency/genetics/metabolismen_US
dc.subjectMammalian/*cytology/*embryology/metabolism/pathology-
dc.titlePlanar cell polarity breaks bilateral symmetry by controlling ciliary positioningen_US
dc.typeArticleen_US
dc.identifier.emailGao, B: gaobo@hku.hken_US
dc.identifier.authorityGao, B=rp02012en_US
dc.identifier.doi10.1038/nature09129en_US
dc.identifier.pmid20562861-
dc.identifier.scopuseid_2-s2.0-77954661192-
dc.identifier.volume466en_US
dc.identifier.issue7304en_US
dc.identifier.spage378en_US
dc.identifier.epage382en_US
dc.identifier.isiWOS:000279867100051-
dc.identifier.f10004040958-

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