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Article: Genome-wide identification of target genes repressed by the zinc finger transcription factor REST/NRSF in the HEK 293 cell line

TitleGenome-wide identification of target genes repressed by the zinc finger transcription factor REST/NRSF in the HEK 293 cell line
Authors
Issue Date2009
PublisherOxford University Press. The Journal's web site is located at http://abbs.oxfordjournals.org/
Citation
Acta Biochimica et Biophysica Sinica, 2009, v. 41 n. 12, p. 1008-1017 How to Cite?
AbstractTranscriptional repression is as important as transcriptional activation in establishing cell-type specific patterns of gene expression. RE1-silencing transcription factor (REST), also known as neuronal restrictive silencing factor (NRSF), is a transcriptional regulator that represses a battery of neuronal differentiation genes in non-neuronal cells or in neural progenitor cells by binding to a specific DNA sequence (repressor element-1/neuron-restrictive silencer element, RE1/NRSE). REST/NRSF functions in the neuronal development are widely studied, however, little is known about target genes in various non-neuronal lineages that may result in cell differentiation. Here, we use RNA interference (RNAi) technology combined with the microarray strategy to identify potential REST/NRSF targets and RE1/NRSEs in human non-neuronal cell line HEK 293. Expression of 54 genes was up-regulated by inhibition of REST/NRSF in the HEK 293 cells according to the microarray experiment and 13 of those were further confirmed by quantitative RT-PCR. Our results confirmed the good confidence and reliability of current research data based on in silico, chromatin immunoprecipitation in combination with microarrays (ChIP-chip), and high-throughput sequencing (ChIP-seq). However, in view of the fact that thousands of genes have been testified or predicted to be recognized by REST/NRSF, our data show that only a few genes among those are directly up-regulated by the interaction of REST/NRSF with RE1/NRSEs sites in gene sequences.
Persistent Identifierhttp://hdl.handle.net/10722/208402
ISSN
2015 Impact Factor: 2.124
2015 SCImago Journal Rankings: 0.881

 

DC FieldValueLanguage
dc.contributor.authorLiu, Z-
dc.contributor.authorLiu, M-
dc.contributor.authorNiu, G-
dc.contributor.authorCheng, Y-
dc.contributor.authorFei, J-
dc.date.accessioned2015-02-25T06:01:24Z-
dc.date.available2015-02-25T06:01:24Z-
dc.date.issued2009-
dc.identifier.citationActa Biochimica et Biophysica Sinica, 2009, v. 41 n. 12, p. 1008-1017-
dc.identifier.issn1672-9145-
dc.identifier.urihttp://hdl.handle.net/10722/208402-
dc.description.abstractTranscriptional repression is as important as transcriptional activation in establishing cell-type specific patterns of gene expression. RE1-silencing transcription factor (REST), also known as neuronal restrictive silencing factor (NRSF), is a transcriptional regulator that represses a battery of neuronal differentiation genes in non-neuronal cells or in neural progenitor cells by binding to a specific DNA sequence (repressor element-1/neuron-restrictive silencer element, RE1/NRSE). REST/NRSF functions in the neuronal development are widely studied, however, little is known about target genes in various non-neuronal lineages that may result in cell differentiation. Here, we use RNA interference (RNAi) technology combined with the microarray strategy to identify potential REST/NRSF targets and RE1/NRSEs in human non-neuronal cell line HEK 293. Expression of 54 genes was up-regulated by inhibition of REST/NRSF in the HEK 293 cells according to the microarray experiment and 13 of those were further confirmed by quantitative RT-PCR. Our results confirmed the good confidence and reliability of current research data based on in silico, chromatin immunoprecipitation in combination with microarrays (ChIP-chip), and high-throughput sequencing (ChIP-seq). However, in view of the fact that thousands of genes have been testified or predicted to be recognized by REST/NRSF, our data show that only a few genes among those are directly up-regulated by the interaction of REST/NRSF with RE1/NRSEs sites in gene sequences.-
dc.languageeng-
dc.publisherOxford University Press. The Journal's web site is located at http://abbs.oxfordjournals.org/-
dc.relation.ispartofActa Biochimica et Biophysica Sinica-
dc.subject.meshDown-Regulation - genetics-
dc.subject.meshGene Silencing-
dc.subject.meshGenome, Human-
dc.subject.meshRepressor Proteins - genetics - metabolism-
dc.subject.meshZinc Fingers-
dc.titleGenome-wide identification of target genes repressed by the zinc finger transcription factor REST/NRSF in the HEK 293 cell lineen_US
dc.typeArticleen_US
dc.identifier.emailNiu, G: wniu@hkucc.hku.hk-
dc.identifier.emailFei, J: jfei@tongji.edu.cn-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1093/abbs/gmp095-
dc.identifier.pmid20011975-
dc.identifier.hkuros180105-
dc.identifier.volume41-
dc.identifier.issue12-
dc.identifier.spage1008-
dc.identifier.epage1017-
dc.publisher.placeUnited States-

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