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Article: Protective immunity induced by peptide mimics to LPS in mice with endotoxic shock

TitleProtective immunity induced by peptide mimics to LPS in mice with endotoxic shock
Authors
KeywordsPeptide mimics
Lipopolysaccharide(LPS)
Endotoxic shock
Protective immunity
Issue Date2008
Citation
Progress in Biochemistry and Biophysics, 2008, v. 35, n. 11, p. 1312-1319 How to Cite?
AbstractTo characterize a mimotop, peptide mimic to epitope on lipopolysaccharide (LPS) which was named as 13L and was expected to induce humoral immune response to LPS and a protective immunity, peptide 13L was synthesized and conjugated to Blue Carrier (BC) as immunogen. Balb/c mice were immunized with peptide 13L - BC conjugate and BC only as control. Anti-LPS antibodies of mice immunized with peptide 13L-BC were detected by ELISA using peptide 13L-BSA as coating antigen. Survival time of mice challenged with S. typhi, and dynamics of MAP/mmHg in mice injected with LPS were observed. The results showed that specific antibodies against S. typhi-LPS 7261 and E. coli-LPS 2630 were elicited in mice immunized with peptide 13L-BC conjugate, and could be boosted by inactive bacterial and LPS. The main isotype of anti-LPS antibodies were IgG2a, IgG2b and IgM, but less IgG1 and IgG3, The survival time of mice infected with S. typhi were (12±1.3) days and (5.3± 0.4) days(P < 0.01) in group immunized with peptide 13L conjugate and with BC, respectively. And the peptide 13L-BC can also elicit protective immunity against endotoxic shock by LPS. The dynamics of MAP/mmHg observed by carotid Artery catheterization showed a significant difference between mice immunized with peptide 13L-BC and mice immunized with BC only in 1, 2,3 and 4 hours after injection of LPS 2630 (P < 0.05, P < 0.01, P < 0.05 and P < 0.01), and injection of LPS 7261 (P > 0.05, P < 0.05, P < 0.05 and P < 0.01). These results suggested that the peptide 13L can mimic the antigenicity of LPS epitopes to induce secondary antibody response like as thymus-dependent antigen, and also can elicit a protective immunity of mice from infection with G- bacteria and endotoxic shock. This peptide mimics could be a new vaccine candidate of LPS.
Persistent Identifierhttp://hdl.handle.net/10722/207909
ISSN
2015 Impact Factor: 0.224
2015 SCImago Journal Rankings: 0.138

 

DC FieldValueLanguage
dc.contributor.authorLuo, HB-
dc.contributor.authorZheng, J-
dc.contributor.authorZhu, P-
dc.contributor.authorHuang, LQ-
dc.contributor.authorFu, N-
dc.date.accessioned2015-01-26T11:46:42Z-
dc.date.available2015-01-26T11:46:42Z-
dc.date.issued2008-
dc.identifier.citationProgress in Biochemistry and Biophysics, 2008, v. 35, n. 11, p. 1312-1319-
dc.identifier.issn1000-3282-
dc.identifier.urihttp://hdl.handle.net/10722/207909-
dc.description.abstractTo characterize a mimotop, peptide mimic to epitope on lipopolysaccharide (LPS) which was named as 13L and was expected to induce humoral immune response to LPS and a protective immunity, peptide 13L was synthesized and conjugated to Blue Carrier (BC) as immunogen. Balb/c mice were immunized with peptide 13L - BC conjugate and BC only as control. Anti-LPS antibodies of mice immunized with peptide 13L-BC were detected by ELISA using peptide 13L-BSA as coating antigen. Survival time of mice challenged with S. typhi, and dynamics of MAP/mmHg in mice injected with LPS were observed. The results showed that specific antibodies against S. typhi-LPS 7261 and E. coli-LPS 2630 were elicited in mice immunized with peptide 13L-BC conjugate, and could be boosted by inactive bacterial and LPS. The main isotype of anti-LPS antibodies were IgG2a, IgG2b and IgM, but less IgG1 and IgG3, The survival time of mice infected with S. typhi were (12±1.3) days and (5.3± 0.4) days(P < 0.01) in group immunized with peptide 13L conjugate and with BC, respectively. And the peptide 13L-BC can also elicit protective immunity against endotoxic shock by LPS. The dynamics of MAP/mmHg observed by carotid Artery catheterization showed a significant difference between mice immunized with peptide 13L-BC and mice immunized with BC only in 1, 2,3 and 4 hours after injection of LPS 2630 (P < 0.05, P < 0.01, P < 0.05 and P < 0.01), and injection of LPS 7261 (P > 0.05, P < 0.05, P < 0.05 and P < 0.01). These results suggested that the peptide 13L can mimic the antigenicity of LPS epitopes to induce secondary antibody response like as thymus-dependent antigen, and also can elicit a protective immunity of mice from infection with G- bacteria and endotoxic shock. This peptide mimics could be a new vaccine candidate of LPS.-
dc.languageeng-
dc.relation.ispartofProgress in Biochemistry and Biophysics-
dc.subjectPeptide mimics-
dc.subjectLipopolysaccharide(LPS)-
dc.subjectEndotoxic shock-
dc.subjectProtective immunity-
dc.titleProtective immunity induced by peptide mimics to LPS in mice with endotoxic shock-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.scopuseid_2-s2.0-59849096402-
dc.identifier.volume35-
dc.identifier.issue11-
dc.identifier.spage1312-
dc.identifier.epage1319-

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