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Article: N-Linked Glycoprotein Analysis Using Dual-Extraction Ultrahigh-Preformance Liquid Chromatography and Electrospray Tandem Mass Spectrometry

TitleN-Linked Glycoprotein Analysis Using Dual-Extraction Ultrahigh-Preformance Liquid Chromatography and Electrospray Tandem Mass Spectrometry
Authors
KeywordsUPLC
2DLC
Glycoproteins
Glycans
Dual extraction
Issue Date2010
PublisherHumana Press, Inc.
Citation
Methods in Molecular Biology, 2010, v. 600, p. 133-143 How to Cite?
AbstractAlthough reverse-phase liquid chromatography (RP-LC) is a common technique for peptide separation in shotgun proteomics and glycoproteomics, it often provides unsatisfactory results for the analysis of glycopeptides and glycans. This bias against glycopeptides makes it difficult to study glycoproteins. By coupling mass spectrometry (MS) with a combination of RP-LC and normal-phase (NP)-LC as an integrated front-end separation system, we demonstrate that effective identification and characterization of both peptides and glycopeptides mixtures, and their constituent glycan structures, can be achieved from a single sample injection event.
Persistent Identifierhttp://hdl.handle.net/10722/207551
ISSN

 

DC FieldValueLanguage
dc.contributor.authorSiu, SO-
dc.contributor.authorLam, MPY-
dc.contributor.authorLau, E-
dc.contributor.authorYeung, WSB-
dc.contributor.authorCox, DM-
dc.contributor.authorChu, IK-
dc.date.accessioned2014-12-31T03:21:11Z-
dc.date.available2014-12-31T03:21:11Z-
dc.date.issued2010-
dc.identifier.citationMethods in Molecular Biology, 2010, v. 600, p. 133-143-
dc.identifier.issn1940-6029-
dc.identifier.urihttp://hdl.handle.net/10722/207551-
dc.description.abstractAlthough reverse-phase liquid chromatography (RP-LC) is a common technique for peptide separation in shotgun proteomics and glycoproteomics, it often provides unsatisfactory results for the analysis of glycopeptides and glycans. This bias against glycopeptides makes it difficult to study glycoproteins. By coupling mass spectrometry (MS) with a combination of RP-LC and normal-phase (NP)-LC as an integrated front-end separation system, we demonstrate that effective identification and characterization of both peptides and glycopeptides mixtures, and their constituent glycan structures, can be achieved from a single sample injection event.-
dc.languageeng-
dc.publisherHumana Press, Inc.-
dc.relation.ispartofMethods in Molecular Biology-
dc.rightsThe final publication is available at Springer via http://dx.doi.org/[insert DOI]-
dc.subjectUPLC-
dc.subject2DLC-
dc.subjectGlycoproteins-
dc.subjectGlycans-
dc.subjectDual extraction-
dc.subject.meshAnimals-
dc.subject.meshCattle-
dc.subject.meshChromatography, High Pressure Liquid - Methods-
dc.subject.meshGlycopeptides - Chemistry-
dc.subject.meshGlycoproteins - Chemistry-
dc.subject.meshHorseradish Peroxidase - Chemistry-
dc.subject.meshMass Spectrometry - Methods-
dc.subject.meshPolysaccharides - Chemistry-
dc.subject.meshProteome-
dc.subject.meshProteomics - Methods-
dc.subject.meshRibonucleases - Chemistry-
dc.subject.meshSerum Albumin - Chemistry-
dc.subject.meshSolvents - Chemistry-
dc.subject.meshSpectrometry, Mass, Electrospray Ionization - Methods-
dc.subject.meshTrypsin - Chemistry-
dc.titleN-Linked Glycoprotein Analysis Using Dual-Extraction Ultrahigh-Preformance Liquid Chromatography and Electrospray Tandem Mass Spectrometry-
dc.typeArticle-
dc.identifier.emailSiu, SO: siuon818@gmail.com-
dc.identifier.emailYeung, WSB: wsbyeung@hkucc.hku.hk-
dc.identifier.emailChu, IK: ivankchu@hku.hk-
dc.identifier.authorityYeung, WSB=rp00331-
dc.identifier.authorityChu, IK=rp00683-
dc.identifier.doi10.1007/978-1-60761-454-8_9-
dc.identifier.pmid19882125-
dc.identifier.scopuseid_2-s2.0-74049116246-
dc.identifier.hkuros173499-
dc.identifier.volume600-
dc.identifier.spage133-
dc.identifier.epage143-
dc.publisher.placeUnited States-
dc.identifier.scopusauthoridSiu, SO=8603087200-
dc.identifier.scopusauthoridLam, MP=35302594800-
dc.identifier.scopusauthoridLau, E=35302963200-
dc.identifier.scopusauthoridYeung, WS=7102370745-
dc.identifier.scopusauthoridCox, DM=35434137900-
dc.identifier.scopusauthoridChu, IK=7103327484-

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