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- Publisher Website: 10.1152/ajpgi.00093.2004
- Scopus: eid_2-s2.0-10844280063
- PMID: 15256360
- WOS: WOS:000225658500005
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Article: Tumor necrosis factor-α stimulates gastric epithelial cell proliferation
Title | Tumor necrosis factor-α stimulates gastric epithelial cell proliferation |
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Authors | |
Keywords | Prostaglandin E 2 Cyclooxygenase Dexamethasone Phospholipase A 2 |
Issue Date | 2005 |
Citation | American Journal of Physiology - Gastrointestinal and Liver Physiology, 2005, v. 288, n. 1 51-1, p. G32-G38 How to Cite? |
Abstract | TNF-α is a cytokine produced during gastric mucosal injury. We examined whether TNF-α could promote mucosal repair by stimulation of epithelial cell proliferation and explored further the underlying mechanisms in a rat gastric mucosal epithelial cell line (RGM-1). TNF-α treatment (1-10 ng/ml) for 12 or 24 h significantly increased cell proliferation but did not induce apoptosis in RGM-1 cells. TNF-α treatment significantly increased cytosolic phospholipase A2 and cyclooxygenase-2 (COX-2) protein expression and PGE 2 level but did not affect the protein levels of EGF, basic fibroblast growth factor, and COX-1 in RGM-1 cells. The mRNA of TNF receptor (TNF-R) 2 but not of TNF-R1 was also increased. Dexamethasone dose dependently inhibited the stimulatory effect of TNF-α on cell proliferation, which was associated with a significant decrease in cellular COX-2 expression and PGE2 level. A selective COX-2 inhibitor 3-(3-fluorophenyl)-4-[4-(methylsulfonyl) phenyl]-5,5-dimethyl- 5H-furan-2-one (DFU) by itself had no effect on basal cell proliferation but significantly reduced the stimulatory effect of TNF-α on RMG-1 cells. Combination of dexamethasone and DFU did not produce an additive effect. PGE 2 significantly reversed the depressive action of dexamethasone on cell proliferation. These results suggest that TNF-α plays a regulatory role in epithelial cell repair in the gastric mucosa via the TNF-α receptor and activation of the arachidonic acid/PG pathway. |
Persistent Identifier | http://hdl.handle.net/10722/207488 |
ISSN | 2023 Impact Factor: 3.9 2023 SCImago Journal Rankings: 1.460 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Jiing, Chyuan Luo | - |
dc.contributor.author | Shin, Vivianyvonne | - |
dc.contributor.author | Ying, HuaYang | - |
dc.contributor.author | Wu, William Ka Kei | - |
dc.contributor.author | Yi, NiYe | - |
dc.contributor.author | So, Wallace Hau Leung | - |
dc.contributor.author | Chang, Fullyoung | - |
dc.contributor.author | Chi, Hincho | - |
dc.date.accessioned | 2014-12-31T01:01:46Z | - |
dc.date.available | 2014-12-31T01:01:46Z | - |
dc.date.issued | 2005 | - |
dc.identifier.citation | American Journal of Physiology - Gastrointestinal and Liver Physiology, 2005, v. 288, n. 1 51-1, p. G32-G38 | - |
dc.identifier.issn | 0193-1857 | - |
dc.identifier.uri | http://hdl.handle.net/10722/207488 | - |
dc.description.abstract | TNF-α is a cytokine produced during gastric mucosal injury. We examined whether TNF-α could promote mucosal repair by stimulation of epithelial cell proliferation and explored further the underlying mechanisms in a rat gastric mucosal epithelial cell line (RGM-1). TNF-α treatment (1-10 ng/ml) for 12 or 24 h significantly increased cell proliferation but did not induce apoptosis in RGM-1 cells. TNF-α treatment significantly increased cytosolic phospholipase A2 and cyclooxygenase-2 (COX-2) protein expression and PGE 2 level but did not affect the protein levels of EGF, basic fibroblast growth factor, and COX-1 in RGM-1 cells. The mRNA of TNF receptor (TNF-R) 2 but not of TNF-R1 was also increased. Dexamethasone dose dependently inhibited the stimulatory effect of TNF-α on cell proliferation, which was associated with a significant decrease in cellular COX-2 expression and PGE2 level. A selective COX-2 inhibitor 3-(3-fluorophenyl)-4-[4-(methylsulfonyl) phenyl]-5,5-dimethyl- 5H-furan-2-one (DFU) by itself had no effect on basal cell proliferation but significantly reduced the stimulatory effect of TNF-α on RMG-1 cells. Combination of dexamethasone and DFU did not produce an additive effect. PGE 2 significantly reversed the depressive action of dexamethasone on cell proliferation. These results suggest that TNF-α plays a regulatory role in epithelial cell repair in the gastric mucosa via the TNF-α receptor and activation of the arachidonic acid/PG pathway. | - |
dc.language | eng | - |
dc.relation.ispartof | American Journal of Physiology - Gastrointestinal and Liver Physiology | - |
dc.subject | Prostaglandin E 2 | - |
dc.subject | Cyclooxygenase | - |
dc.subject | Dexamethasone | - |
dc.subject | Phospholipase A 2 | - |
dc.title | Tumor necrosis factor-α stimulates gastric epithelial cell proliferation | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1152/ajpgi.00093.2004 | - |
dc.identifier.pmid | 15256360 | - |
dc.identifier.scopus | eid_2-s2.0-10844280063 | - |
dc.identifier.volume | 288 | - |
dc.identifier.issue | 1 51-1 | - |
dc.identifier.spage | G32 | - |
dc.identifier.epage | G38 | - |
dc.identifier.isi | WOS:000225658500005 | - |
dc.identifier.issnl | 0193-1857 | - |