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Article: Modulation of COX2 and hTERT expression by Photodynamic Therapy in human colon cancer cells

TitleModulation of COX2 and hTERT expression by Photodynamic Therapy in human colon cancer cells
Authors
KeywordsPhotodynamic Therapy
Colon cancer
Modulation
Cancer
Flow cytometry
Issue Date2009
PublisherSPIE - International Society for Optical Engineering. The Journal's web site is located at http://spie.org/x1848.xml?WT.svl=mddp2
Citation
Proceedings of SPIE, 2009, v. 7380, p. article no. 738065 How to Cite?
AbstractPhotodynamic therapy (PDT) was employed as a cancer therapy with photosensitizer (PS)-loaded cancer cells, eradicated by the reactive oxygen species after light activation. Cyclo-oxygenase 2 (COX2) is an enzyme expressed in 80% of colon adenocarcinoma and is one of the targets for effective cancer treatment. There is also uprising evidence that the human telomerase reverse transcriptase (hTERT), a catalytic component of telomerase, is reported as a promising indicator for monitoring cancer treatment. In this study, NPe6 mediated PDT on COX2 induced apoptosis in HT-29 was investigated. The cell cycle changes was analysed by flow cytometry and the hTERT expression at pre and post PDT was evaluated at transcription level by Taqman real time PCR. NPe6-PDT in HT-29 cells demonstrated anti-proliferating effect in a drug and light dose dependent manner. LD50 was achieved at 16μg/mL and 2J/cm2 at 4 hour-post treatment with a significant down-regulation of COX2 expression at LD30 and LD50 by immunohistochemical staining (IHC) (p<0.05, One-Way ANOVA). Membrane blebbing was detected in over 60% of cells. 35.2% of treated cells arrested in S-phase at LD50 after 24 hours by flow cytometry. A 0.25- and 0.6-fold down-regulation of hTERT mRNA expression was achieved at LD30 and LD50 respectively by TaqMan real-time PCR. To summarize, NPe6 mediated PDT down-regulated COX2 expression and triggered cell apoptosis. The hTERT can serve as an indicative marker for monitoring NPe6-PDT cancer treatment efficacy.
Persistent Identifierhttp://hdl.handle.net/10722/207481
ISSN

 

DC FieldValueLanguage
dc.contributor.authorYow, CLMN-
dc.contributor.authorChu, ESM-
dc.date.accessioned2014-12-29T08:15:51Z-
dc.date.available2014-12-29T08:15:51Z-
dc.date.issued2009-
dc.identifier.citationProceedings of SPIE, 2009, v. 7380, p. article no. 738065-
dc.identifier.issn0277-786X-
dc.identifier.urihttp://hdl.handle.net/10722/207481-
dc.description.abstractPhotodynamic therapy (PDT) was employed as a cancer therapy with photosensitizer (PS)-loaded cancer cells, eradicated by the reactive oxygen species after light activation. Cyclo-oxygenase 2 (COX2) is an enzyme expressed in 80% of colon adenocarcinoma and is one of the targets for effective cancer treatment. There is also uprising evidence that the human telomerase reverse transcriptase (hTERT), a catalytic component of telomerase, is reported as a promising indicator for monitoring cancer treatment. In this study, NPe6 mediated PDT on COX2 induced apoptosis in HT-29 was investigated. The cell cycle changes was analysed by flow cytometry and the hTERT expression at pre and post PDT was evaluated at transcription level by Taqman real time PCR. NPe6-PDT in HT-29 cells demonstrated anti-proliferating effect in a drug and light dose dependent manner. LD50 was achieved at 16μg/mL and 2J/cm2 at 4 hour-post treatment with a significant down-regulation of COX2 expression at LD30 and LD50 by immunohistochemical staining (IHC) (p<0.05, One-Way ANOVA). Membrane blebbing was detected in over 60% of cells. 35.2% of treated cells arrested in S-phase at LD50 after 24 hours by flow cytometry. A 0.25- and 0.6-fold down-regulation of hTERT mRNA expression was achieved at LD30 and LD50 respectively by TaqMan real-time PCR. To summarize, NPe6 mediated PDT down-regulated COX2 expression and triggered cell apoptosis. The hTERT can serve as an indicative marker for monitoring NPe6-PDT cancer treatment efficacy.-
dc.languageeng-
dc.publisherSPIE - International Society for Optical Engineering. The Journal's web site is located at http://spie.org/x1848.xml?WT.svl=mddp2-
dc.relation.ispartofProceedings of SPIE-
dc.rightsProceedings of SPIE. Copyright © SPIE - International Society for Optical Engineering.-
dc.rightsCopyright notice format: Copyright 2009 (year) Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectPhotodynamic Therapy-
dc.subjectColon cancer-
dc.subjectModulation-
dc.subjectCancer-
dc.subjectFlow cytometry-
dc.titleModulation of COX2 and hTERT expression by Photodynamic Therapy in human colon cancer cellsen_US
dc.typeArticleen_US
dc.identifier.emailChu, ESM: elliecsm@hku.hk, htellie@inet.polyu.edu.hk-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1117/12.823084-
dc.identifier.hkuros166069-
dc.identifier.volume7380-
dc.identifier.spagearticle no. 738065-
dc.identifier.epagearticle no. 738065-
dc.publisher.placeUnited States-

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