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Article: A Mechanistic Study of the Electron Capture Dissociation of Oligonucleotides

TitleA Mechanistic Study of the Electron Capture Dissociation of Oligonucleotides
Authors
Issue Date2009
Citation
Journal of the American Society for Mass Spectrometry, 2009, v. 20, n. 2, p. 213-226 How to Cite?
AbstractElectron capture dissociation (ECD) of a series of custom-synthesized oligonucleotide pentamers was performed in a Fourier-transform mass spectrometer with a conventional filament-type electron gun. Dissociation of oligonucleotide ions by electron capture generates primarily w/d-type and z/a-type ions with and without the loss of a nucleobase fragment ions. Minor yields of radical [z/a + H]· fragment ions were also observed in many cases. It is interesting to note that some nucleoside-like fragment ions and protonated nucleobase ions (except thymine-related nucleobases and nucleoside-like fragments) were observed in most ECD spectra. The formation of these low-mass fragment ions was tentatively attributed to the secondary fragmentation of the radical [z + H]· fragment ions. From the ECD tandem mass spectra of a series of C/T based binary oligonucleotide ions, including d(CTCTC), d(CTTTC), d(TCCCT), d(CCCCT), and d(TCCCC), it was clearly demonstrated that the formation of many sequence ions was sensitive to the position of cytosine (or the position of charge carrier). The findings of this work support a notion that the ECD of protonated oligonucleotide molecules is charge-directed with the electron being captured by the protonated nucleobase. © 2009 American Society for Mass Spectrometry.
Persistent Identifierhttp://hdl.handle.net/10722/206287
ISSN
2015 Impact Factor: 3.031
2015 SCImago Journal Rankings: 1.322
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChan, Tak Wah Dominic-
dc.contributor.authorChoy, Man Fai-
dc.contributor.authorChan, Wai Yi Kelly-
dc.contributor.authorFung, Yi Man Eva-
dc.date.accessioned2014-10-22T01:25:34Z-
dc.date.available2014-10-22T01:25:34Z-
dc.date.issued2009-
dc.identifier.citationJournal of the American Society for Mass Spectrometry, 2009, v. 20, n. 2, p. 213-226-
dc.identifier.issn1044-0305-
dc.identifier.urihttp://hdl.handle.net/10722/206287-
dc.description.abstractElectron capture dissociation (ECD) of a series of custom-synthesized oligonucleotide pentamers was performed in a Fourier-transform mass spectrometer with a conventional filament-type electron gun. Dissociation of oligonucleotide ions by electron capture generates primarily w/d-type and z/a-type ions with and without the loss of a nucleobase fragment ions. Minor yields of radical [z/a + H]· fragment ions were also observed in many cases. It is interesting to note that some nucleoside-like fragment ions and protonated nucleobase ions (except thymine-related nucleobases and nucleoside-like fragments) were observed in most ECD spectra. The formation of these low-mass fragment ions was tentatively attributed to the secondary fragmentation of the radical [z + H]· fragment ions. From the ECD tandem mass spectra of a series of C/T based binary oligonucleotide ions, including d(CTCTC), d(CTTTC), d(TCCCT), d(CCCCT), and d(TCCCC), it was clearly demonstrated that the formation of many sequence ions was sensitive to the position of cytosine (or the position of charge carrier). The findings of this work support a notion that the ECD of protonated oligonucleotide molecules is charge-directed with the electron being captured by the protonated nucleobase. © 2009 American Society for Mass Spectrometry.-
dc.languageeng-
dc.relation.ispartofJournal of the American Society for Mass Spectrometry-
dc.titleA Mechanistic Study of the Electron Capture Dissociation of Oligonucleotides-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jasms.2008.08.018-
dc.identifier.pmid18842427-
dc.identifier.scopuseid_2-s2.0-58249092025-
dc.identifier.volume20-
dc.identifier.issue2-
dc.identifier.spage213-
dc.identifier.epage226-
dc.identifier.isiWOS:000263004500006-

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