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Article: Bifurcating Fragmentation Behavior of Gas-Phase Tryptic Peptide Dications in Collisional Activation

TitleBifurcating Fragmentation Behavior of Gas-Phase Tryptic Peptide Dications in Collisional Activation
Authors
Issue Date2008
Citation
Journal of the American Society for Mass Spectrometry, 2008, v. 19, n. 12, p. 1755-1763 How to Cite?
AbstractCollision-activated dissociation (CAD) of tryptic peptides is a cornerstone of mass spectrometry-based proteomics research. Principal component analysis of a database containing 15,000 high-resolution CAD mass spectra of gas-phase tryptic peptide dications revealed that they fall into two classes with a good separation between the classes. The main factor determining the class identity is the relative abundance of the peptide bond cleavage after the first two N-terminal residues. A possible scenario explaining this bifurcation involves trans- to cis-isomerization of the N-terminal peptide bond, which facilitates solvation of the N-terminal charge on the second backbone amide and formation of stable b2 ions in the form of protonated diketopiperazines. Evidence supporting this scenario is derived from statistical analysis of the high-resolution CAD MS/MS database. It includes the observation of the strong deficit of a3 ions and anomalous amino acid preferences for b2 ion formation. © 2008 American Society for Mass Spectrometry.
Persistent Identifierhttp://hdl.handle.net/10722/206286
ISSN
2015 Impact Factor: 3.031
2015 SCImago Journal Rankings: 1.322
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorSavitski, Mikhail M.-
dc.contributor.authorFälth, Maria-
dc.contributor.authorFung, Yi Man Eva-
dc.contributor.authorAdams, Christopher M.-
dc.contributor.authorZubarev, Roman A.-
dc.date.accessioned2014-10-22T01:25:34Z-
dc.date.available2014-10-22T01:25:34Z-
dc.date.issued2008-
dc.identifier.citationJournal of the American Society for Mass Spectrometry, 2008, v. 19, n. 12, p. 1755-1763-
dc.identifier.issn1044-0305-
dc.identifier.urihttp://hdl.handle.net/10722/206286-
dc.description.abstractCollision-activated dissociation (CAD) of tryptic peptides is a cornerstone of mass spectrometry-based proteomics research. Principal component analysis of a database containing 15,000 high-resolution CAD mass spectra of gas-phase tryptic peptide dications revealed that they fall into two classes with a good separation between the classes. The main factor determining the class identity is the relative abundance of the peptide bond cleavage after the first two N-terminal residues. A possible scenario explaining this bifurcation involves trans- to cis-isomerization of the N-terminal peptide bond, which facilitates solvation of the N-terminal charge on the second backbone amide and formation of stable b2 ions in the form of protonated diketopiperazines. Evidence supporting this scenario is derived from statistical analysis of the high-resolution CAD MS/MS database. It includes the observation of the strong deficit of a3 ions and anomalous amino acid preferences for b2 ion formation. © 2008 American Society for Mass Spectrometry.-
dc.languageeng-
dc.relation.ispartofJournal of the American Society for Mass Spectrometry-
dc.titleBifurcating Fragmentation Behavior of Gas-Phase Tryptic Peptide Dications in Collisional Activation-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jasms.2008.08.003-
dc.identifier.pmid18799320-
dc.identifier.scopuseid_2-s2.0-56949103268-
dc.identifier.volume19-
dc.identifier.issue12-
dc.identifier.spage1755-
dc.identifier.epage1763-
dc.identifier.isiWOS:000261808900005-

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