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- Publisher Website: 10.1096/fj.11-184622
- Scopus: eid_2-s2.0-80051704067
- PMID: 21507899
- WOS: WOS:000293337800009
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Article: The sarcoplasmic reticulum luminal thiol oxidase ERO1 regulates cardiomyocyte excitation-coupled calcium release and response to hemodynamic load
Title | The sarcoplasmic reticulum luminal thiol oxidase ERO1 regulates cardiomyocyte excitation-coupled calcium release and response to hemodynamic load |
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Authors | |
Keywords | Disulfide bonds Heart failure Endoplasmic reticulum |
Issue Date | 2011 |
Citation | FASEB Journal, 2011, v. 25, n. 8, p. 2583-2591 How to Cite? |
Abstract | Two related ER oxidation 1 (ERO1) proteins, ERO1α and ERO1β, dynamically regulate the redox environment in the mammalian endoplasmic reticulum (ER). Redox changes in cysteine residues on intralumenal loops of calcium release and reuptake channels have been implicated in altered calcium release and reuptake. These findings led us to hypothesize that altered ERO1 activity may affect cardiac functions that are dependent on intracellular calcium flux. We established mouse lines with loss of function insertion mutations in Ero1l and Ero1lb encoding ERO1α and ERO1β. The peak amplitude of calcium transients in homozygous Ero1α mutant adult cardiomyocytes was reduced to 42.0 ± 2.2% (n=10, P≤0.01) of values recorded in wild-type cardiomyocytes. Decreased ERO1 activity blunted cardiomyocyte inotropic response to adrenergic stimulation and sensitized mice to adrenergic blockade. Whereas all 12 wild-type mice survived challenge with 4 mg/kg esmolol, 6 of 8 compound Ero1l and Ero1lb mutant mice succumbed to this level of β adrenergic blockade (P≤0.01). In addition, mice lacking ERO1α were partially protected against progressive heart failure in a transaortic constriction model [at 10 wk postprocedure, fractional shortening was 0.31±0.02 in the mutant (n=20) vs. 0.23±0.03 in the wild type (n=18); P≤0.01]. These findings establish a role for ERO1 in calcium homeostasis and suggest that modifying the lumenal redox environment may affect the progression of heart failure. |
Persistent Identifier | http://hdl.handle.net/10722/205747 |
ISSN | 2023 Impact Factor: 4.4 2023 SCImago Journal Rankings: 1.412 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Chin, Kingtung | - |
dc.contributor.author | Kang, Guoxin | - |
dc.contributor.author | Qu, Jiaxiang | - |
dc.contributor.author | Gardner, Lawrence B. | - |
dc.contributor.author | Coetzee, William A. | - |
dc.contributor.author | Zito, Ester | - |
dc.contributor.author | Fishman, Glenn I. | - |
dc.contributor.author | Ron, David | - |
dc.date.accessioned | 2014-10-06T08:02:18Z | - |
dc.date.available | 2014-10-06T08:02:18Z | - |
dc.date.issued | 2011 | - |
dc.identifier.citation | FASEB Journal, 2011, v. 25, n. 8, p. 2583-2591 | - |
dc.identifier.issn | 0892-6638 | - |
dc.identifier.uri | http://hdl.handle.net/10722/205747 | - |
dc.description.abstract | Two related ER oxidation 1 (ERO1) proteins, ERO1α and ERO1β, dynamically regulate the redox environment in the mammalian endoplasmic reticulum (ER). Redox changes in cysteine residues on intralumenal loops of calcium release and reuptake channels have been implicated in altered calcium release and reuptake. These findings led us to hypothesize that altered ERO1 activity may affect cardiac functions that are dependent on intracellular calcium flux. We established mouse lines with loss of function insertion mutations in Ero1l and Ero1lb encoding ERO1α and ERO1β. The peak amplitude of calcium transients in homozygous Ero1α mutant adult cardiomyocytes was reduced to 42.0 ± 2.2% (n=10, P≤0.01) of values recorded in wild-type cardiomyocytes. Decreased ERO1 activity blunted cardiomyocyte inotropic response to adrenergic stimulation and sensitized mice to adrenergic blockade. Whereas all 12 wild-type mice survived challenge with 4 mg/kg esmolol, 6 of 8 compound Ero1l and Ero1lb mutant mice succumbed to this level of β adrenergic blockade (P≤0.01). In addition, mice lacking ERO1α were partially protected against progressive heart failure in a transaortic constriction model [at 10 wk postprocedure, fractional shortening was 0.31±0.02 in the mutant (n=20) vs. 0.23±0.03 in the wild type (n=18); P≤0.01]. These findings establish a role for ERO1 in calcium homeostasis and suggest that modifying the lumenal redox environment may affect the progression of heart failure. | - |
dc.language | eng | - |
dc.relation.ispartof | FASEB Journal | - |
dc.subject | Disulfide bonds | - |
dc.subject | Heart failure | - |
dc.subject | Endoplasmic reticulum | - |
dc.title | The sarcoplasmic reticulum luminal thiol oxidase ERO1 regulates cardiomyocyte excitation-coupled calcium release and response to hemodynamic load | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1096/fj.11-184622 | - |
dc.identifier.pmid | 21507899 | - |
dc.identifier.scopus | eid_2-s2.0-80051704067 | - |
dc.identifier.volume | 25 | - |
dc.identifier.issue | 8 | - |
dc.identifier.spage | 2583 | - |
dc.identifier.epage | 2591 | - |
dc.identifier.eissn | 1530-6860 | - |
dc.identifier.isi | WOS:000293337800009 | - |
dc.identifier.issnl | 0892-6638 | - |