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Article: Calpain and N-methyl-D-aspartate (NMDA)-induced excitotoxicity in rat retinas

TitleCalpain and N-methyl-D-aspartate (NMDA)-induced excitotoxicity in rat retinas
Authors
KeywordsNMDA
Calpain
Ganglion cell
Apoptosis
Proteinases
Issue Date2005
Citation
Brain Research, 2005, v. 1046, n. 1-2, p. 207-215 How to Cite?
AbstractCalpain-mediated proteolysis has been implicated as a major process in neuronal cell death in both acute insults and the chronic neurodegenerative disorders in the central nerves system. However, activation of calpain also plays a protective function in the early phase of excitotoxic neuronal death. The exact role of calpains in neuronal death and recovery after exposure to N-methyl-d-aspartate (NMDA) is not clearly known. The purpose of present study was to examine the involvement of μ- and m-calpain in NMDA-induced excitotoxicity in the adult rat retina. Increased immunoreactivity of μ-calpain was noted in RGC layer cells and in the inner nuclear layer with maximal expression at 12 h after NMDA injection. This was further confirmed with Western blotting. TdT-mediated biotin-dUTP nick end labeling (TUNEL) positive cells in the inner retina co-localized with moderate or intense μ-calpain immunoreactivity. In contrast, there was no remarkable change in m-calpain immunoreactivity at any time point after NMDA injection. Simultaneous injection of 2 nmol of a calpain inhibitor (calpain inhibitor II) significantly reduced the number of TUNEL-positive cells in the inner retina at 18 h after NMDA injection and preserved RGC-like cells counted at 7 days after injection. The results of this study showed that μ-calpain may be involved in mediating NMDA-induced excitotoxicity in the rat retina and calpain inhibitors may play a therapeutic role in NMDA related disease. © 2005 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/205695
ISSN
2015 Impact Factor: 2.561
2015 SCImago Journal Rankings: 1.351
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChiu, Kin-
dc.contributor.authorTim, Tak Lam-
dc.contributor.authorLi, Winnie Wai Ying-
dc.contributor.authorCaprioli, Joseph-
dc.contributor.authorKwong, Jacky Man Kwong-
dc.date.accessioned2014-10-06T08:02:13Z-
dc.date.available2014-10-06T08:02:13Z-
dc.date.issued2005-
dc.identifier.citationBrain Research, 2005, v. 1046, n. 1-2, p. 207-215-
dc.identifier.issn0006-8993-
dc.identifier.urihttp://hdl.handle.net/10722/205695-
dc.description.abstractCalpain-mediated proteolysis has been implicated as a major process in neuronal cell death in both acute insults and the chronic neurodegenerative disorders in the central nerves system. However, activation of calpain also plays a protective function in the early phase of excitotoxic neuronal death. The exact role of calpains in neuronal death and recovery after exposure to N-methyl-d-aspartate (NMDA) is not clearly known. The purpose of present study was to examine the involvement of μ- and m-calpain in NMDA-induced excitotoxicity in the adult rat retina. Increased immunoreactivity of μ-calpain was noted in RGC layer cells and in the inner nuclear layer with maximal expression at 12 h after NMDA injection. This was further confirmed with Western blotting. TdT-mediated biotin-dUTP nick end labeling (TUNEL) positive cells in the inner retina co-localized with moderate or intense μ-calpain immunoreactivity. In contrast, there was no remarkable change in m-calpain immunoreactivity at any time point after NMDA injection. Simultaneous injection of 2 nmol of a calpain inhibitor (calpain inhibitor II) significantly reduced the number of TUNEL-positive cells in the inner retina at 18 h after NMDA injection and preserved RGC-like cells counted at 7 days after injection. The results of this study showed that μ-calpain may be involved in mediating NMDA-induced excitotoxicity in the rat retina and calpain inhibitors may play a therapeutic role in NMDA related disease. © 2005 Elsevier B.V. All rights reserved.-
dc.languageeng-
dc.relation.ispartofBrain Research-
dc.subjectNMDA-
dc.subjectCalpain-
dc.subjectGanglion cell-
dc.subjectApoptosis-
dc.subjectProteinases-
dc.titleCalpain and N-methyl-D-aspartate (NMDA)-induced excitotoxicity in rat retinas-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.brainres.2005.04.016-
dc.identifier.pmid15878434-
dc.identifier.scopuseid_2-s2.0-20144385366-
dc.identifier.volume1046-
dc.identifier.issue1-2-
dc.identifier.spage207-
dc.identifier.epage215-
dc.identifier.isiWOS:000229898600024-

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