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Article: Serotonin enhances megakaryopoiesis and proplatelet formation via p-Erk1/2 and F-actin reorganization.

TitleSerotonin enhances megakaryopoiesis and proplatelet formation via p-Erk1/2 and F-actin reorganization.
Authors
Issue Date2014
PublisherAlphaMed Press. The Journal's web site is located at http://www.stemcells.com
Citation
Stem Cells, 2014, v. 32 n. 11, p. 2973-2982 How to Cite?
AbstractOur previous studies have shown that serotonin (5-HT) is a growth factor for hematopoietic stem/progenitor cells. In this study, we proposed a possible mechanism: 5-HT may enhance megakaryopoiesis and proplatelet formation via Erk1/2 pathway and cytoskeleton reorganization. Here, 5-HT2B R was first identified in megakaryocytic cells. 5-HT also promoted the megakaryocytes (MKs) proliferation and reduced the cell apoptosis via the activation of 5-HT2B R and Akt pathway. The effects were reduced by the 5-HT2B R inhibitor ketanserin. The effect of 5-HT on proplatelet formation in bone marrow MKs were further confirmed: the 5-HT treated group had more proplatelet bearing MKs compared with the control group. To determine whether 5-HT has effects on cytoskeleton reorganization of MKs, and whether these effects could be reduced by ketanserin or Erk1/2 inhibitor PD98059, MKs were stained with the F-actin specific binder rhodamine-phalloidin. The polymerized actin level was lower in the control group than the 5-HT group and was distributed throughout the cytoplasm with occasional aggregations. Our data demonstrated that Erk1/2 was activated in MKs treated with 5-HT. This study suggests that 5-HT has a potent effect on platelet formation and this effect is likely mediated via 5HT2B R with subsequent activation of p-Erk1/2 and consequent F-actin reorganization and proplatelet formation. We also demonstrated that melatonin, the metabolite of 5-HT, exerts a protective effect on MK and platelet recovery in the irradiated mouse model. This study suggested that 5-HT plays an important role in platelet formation via 5HT2B R, p-Erk1/2 and F-actin reorganization.
Persistent Identifierhttp://hdl.handle.net/10722/205574

 

DC FieldValueLanguage
dc.contributor.authorYe, JYen_US
dc.contributor.authorLiang, EYen_US
dc.contributor.authorCheng, YSen_US
dc.contributor.authorChan, GCFen_US
dc.contributor.authorDing, Yen_US
dc.contributor.authorMeng, FYen_US
dc.contributor.authorNg, MHen_US
dc.contributor.authorChong, BHen_US
dc.contributor.authorLian, Qen_US
dc.contributor.authorYang, Men_US
dc.date.accessioned2014-09-20T04:00:22Z-
dc.date.available2014-09-20T04:00:22Z-
dc.date.issued2014-
dc.identifier.citationStem Cells, 2014, v. 32 n. 11, p. 2973-2982en_US
dc.identifier.urihttp://hdl.handle.net/10722/205574-
dc.description.abstractOur previous studies have shown that serotonin (5-HT) is a growth factor for hematopoietic stem/progenitor cells. In this study, we proposed a possible mechanism: 5-HT may enhance megakaryopoiesis and proplatelet formation via Erk1/2 pathway and cytoskeleton reorganization. Here, 5-HT2B R was first identified in megakaryocytic cells. 5-HT also promoted the megakaryocytes (MKs) proliferation and reduced the cell apoptosis via the activation of 5-HT2B R and Akt pathway. The effects were reduced by the 5-HT2B R inhibitor ketanserin. The effect of 5-HT on proplatelet formation in bone marrow MKs were further confirmed: the 5-HT treated group had more proplatelet bearing MKs compared with the control group. To determine whether 5-HT has effects on cytoskeleton reorganization of MKs, and whether these effects could be reduced by ketanserin or Erk1/2 inhibitor PD98059, MKs were stained with the F-actin specific binder rhodamine-phalloidin. The polymerized actin level was lower in the control group than the 5-HT group and was distributed throughout the cytoplasm with occasional aggregations. Our data demonstrated that Erk1/2 was activated in MKs treated with 5-HT. This study suggests that 5-HT has a potent effect on platelet formation and this effect is likely mediated via 5HT2B R with subsequent activation of p-Erk1/2 and consequent F-actin reorganization and proplatelet formation. We also demonstrated that melatonin, the metabolite of 5-HT, exerts a protective effect on MK and platelet recovery in the irradiated mouse model. This study suggested that 5-HT plays an important role in platelet formation via 5HT2B R, p-Erk1/2 and F-actin reorganization.en_US
dc.languageengen_US
dc.publisherAlphaMed Press. The Journal's web site is located at http://www.stemcells.comen_US
dc.relation.ispartofStem Cellsen_US
dc.titleSerotonin enhances megakaryopoiesis and proplatelet formation via p-Erk1/2 and F-actin reorganization.en_US
dc.typeArticleen_US
dc.identifier.emailChan, GCF: gcfchan@hku.hken_US
dc.identifier.emailDing, Y: dingnash@hku.hken_US
dc.identifier.emailLian, Q: qzlian@hkucc.hku.hken_US
dc.identifier.emailYang, M: yangmo@hkucc.hku.hken_US
dc.identifier.authorityChan, GCF=rp00431en_US
dc.identifier.authorityLian, Q=rp00267en_US
dc.identifier.doi10.1002/stem.1777en_US
dc.identifier.hkuros239229en_US

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