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Article: Effect of the PB2 and M Genes on the Replication of H6 Influenza Virus in Chickens

TitleEffect of the PB2 and M Genes on the Replication of H6 Influenza Virus in Chickens
Authors
Issue Date2014
PublisherHindawi Publishing Corporation. The Journal's web site is located at http://www.hindawi.com/journals/irt
Citation
Influenza Research and Treatment, 2014, v. 2014, article no. 547839 How to Cite?
AbstractH6 subtype influenza viruses are commonly isolated from wild aquatic birds. However, limited information is available regarding H6 influenza virus isolated from chickens. We compared the viral genome segment between A/chicken/Hong Kong/W312/97 (H6N1), which was able to grow in chicken trachea, and A/duck/Shantou/5540/01 (H6N2), which was isolated from wild aquatic duck, to explore the factors for effective replication in chicken. When chickens were inoculated with reassortants (W312 background), the replication of viruses with PB2 and M genes derived from the duck strain was significantly reduced. Chimeras of PB2 and M proteins, encoding the C-terminal region of the PB2 protein and the M2 protein from W312, were required for efficient replication in canine-derived (MDCK) cells and in chicken trachea. These results indicate that host range may be determined by some types of internal proteins such as PB2 and M2, as well as by surface glycoprotein like hemagglutinin.
Persistent Identifierhttp://hdl.handle.net/10722/205475
PubMed Central ID

 

DC FieldValueLanguage
dc.contributor.authorOzaki, Hen_US
dc.contributor.authorGuan, Yen_US
dc.contributor.authorPeiris, JSMen_US
dc.contributor.authorWebster, Ren_US
dc.contributor.authorTakada, Aen_US
dc.contributor.authorWebby, Ren_US
dc.date.accessioned2014-09-20T02:36:21Z-
dc.date.available2014-09-20T02:36:21Z-
dc.date.issued2014en_US
dc.identifier.citationInfluenza Research and Treatment, 2014, v. 2014, article no. 547839en_US
dc.identifier.urihttp://hdl.handle.net/10722/205475-
dc.description.abstractH6 subtype influenza viruses are commonly isolated from wild aquatic birds. However, limited information is available regarding H6 influenza virus isolated from chickens. We compared the viral genome segment between A/chicken/Hong Kong/W312/97 (H6N1), which was able to grow in chicken trachea, and A/duck/Shantou/5540/01 (H6N2), which was isolated from wild aquatic duck, to explore the factors for effective replication in chicken. When chickens were inoculated with reassortants (W312 background), the replication of viruses with PB2 and M genes derived from the duck strain was significantly reduced. Chimeras of PB2 and M proteins, encoding the C-terminal region of the PB2 protein and the M2 protein from W312, were required for efficient replication in canine-derived (MDCK) cells and in chicken trachea. These results indicate that host range may be determined by some types of internal proteins such as PB2 and M2, as well as by surface glycoprotein like hemagglutinin.en_US
dc.languageengen_US
dc.publisherHindawi Publishing Corporation. The Journal's web site is located at http://www.hindawi.com/journals/irt-
dc.relation.ispartofInfluenza Research and Treatmenten_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titleEffect of the PB2 and M Genes on the Replication of H6 Influenza Virus in Chickensen_US
dc.typeArticleen_US
dc.identifier.emailGuan, Y: yguan@hkucc.hku.hken_US
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hken_US
dc.identifier.authorityGuan, Y=rp00397en_US
dc.identifier.authorityPeiris, JSM=rp00410en_US
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1155/2014/547839en_US
dc.identifier.pmid24696782-
dc.identifier.pmcidPMC3948651-
dc.identifier.hkuros239208en_US
dc.identifier.volume2014en_US

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