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Conference Paper: Genomic insights into high exopolysaccharideproducing dairy starter bacterium Streptococcus thermophilus ASCC 1275

TitleGenomic insights into high exopolysaccharideproducing dairy starter bacterium Streptococcus thermophilus ASCC 1275
Authors
Keywordsgenome sequencing
EPS biosynthesis
Streptococcus thermophilus
Issue Date2014
PublisherAmerican Society of Animal Science. The Journal's web site is located at http://jas.fass.org
Citation
Joint Annual Meeting (JAM) of the American Dairy Science Association (ADSA), the American Society of Animal Science (ASAS), and the Canadian Society of Animal Science (CSAS), Kansas City, Missouri, USA, 20-24 July. In the Journal of Animal Science, 2014, v. 92 n. E-Suppl. 2, p. 164, abstract no. 0332 How to Cite?
AbstractStreptococcus thermophilus ASCC 1275 (ST 1275) is a typical dairy starter bacterium and produces the highest known amount (1,000 mg/L) of exopolysaccharide (EPS) in milk within this species. This organism produces both capsular and ropy EPS and possesses textural modifying properties for yogurt and cheese. In this study, de novo shotgun paired-end pyrosequencing was applied to complete the whole genome of ST 1275. The genome size of ST 1275, a plasmid-free bacterium, was 1.85 Mbp with an average GC content of 39.1%. A novel eps gene cluster for EPS assembly containing two-pair genes of epsC- epsD for determining the chain length of EPS was found in ST 1275 genome, which confirms that ST 1275 produces two types of EPSs as found in our previous studies. Compared with other sequenced S. thermophilus strains, ST 1275 possessed the lowest numbers of 5 rRNA operons and 55 tRNAs suggesting that this organism may have a more effective protein synthesis machinery. The highest number of four separate CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated) loci was found in ST 1275 genome indicating that this organism may have a better adaptive immunity against various bacteriophage infections. Further analysis including carbohydrate utilization, effective proteolytic system, sophisticated stress response systems and defense systems in ST 1275 was performed to provide genomic insights into its adaptation to milk and as a cell factory for EPS production during milk fermentation. The elucidation of ST 1275 genome makes this organism as a model dairy starter bacterium for the research of high EPS yield and capsular/ropy EPS producer from the species of S. thermophilus.
DescriptionConference Theme: Linking animal science and animal agriculture: Meeting the global demands of 2050
Graduate Student Competition: ADSA Dairy Foods Oral
Persistent Identifierhttp://hdl.handle.net/10722/205072
ISSN
2015 Impact Factor: 2.014
2015 SCImago Journal Rankings: 1.377

 

DC FieldValueLanguage
dc.contributor.authorShah, Nen_US
dc.contributor.authorWu, Qen_US
dc.contributor.authorTun, HMen_US
dc.date.accessioned2014-09-20T01:20:00Z-
dc.date.available2014-09-20T01:20:00Z-
dc.date.issued2014en_US
dc.identifier.citationJoint Annual Meeting (JAM) of the American Dairy Science Association (ADSA), the American Society of Animal Science (ASAS), and the Canadian Society of Animal Science (CSAS), Kansas City, Missouri, USA, 20-24 July. In the Journal of Animal Science, 2014, v. 92 n. E-Suppl. 2, p. 164, abstract no. 0332en_US
dc.identifier.issn0021-8812-
dc.identifier.urihttp://hdl.handle.net/10722/205072-
dc.descriptionConference Theme: Linking animal science and animal agriculture: Meeting the global demands of 2050-
dc.descriptionGraduate Student Competition: ADSA Dairy Foods Oral-
dc.description.abstractStreptococcus thermophilus ASCC 1275 (ST 1275) is a typical dairy starter bacterium and produces the highest known amount (1,000 mg/L) of exopolysaccharide (EPS) in milk within this species. This organism produces both capsular and ropy EPS and possesses textural modifying properties for yogurt and cheese. In this study, de novo shotgun paired-end pyrosequencing was applied to complete the whole genome of ST 1275. The genome size of ST 1275, a plasmid-free bacterium, was 1.85 Mbp with an average GC content of 39.1%. A novel eps gene cluster for EPS assembly containing two-pair genes of epsC- epsD for determining the chain length of EPS was found in ST 1275 genome, which confirms that ST 1275 produces two types of EPSs as found in our previous studies. Compared with other sequenced S. thermophilus strains, ST 1275 possessed the lowest numbers of 5 rRNA operons and 55 tRNAs suggesting that this organism may have a more effective protein synthesis machinery. The highest number of four separate CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated) loci was found in ST 1275 genome indicating that this organism may have a better adaptive immunity against various bacteriophage infections. Further analysis including carbohydrate utilization, effective proteolytic system, sophisticated stress response systems and defense systems in ST 1275 was performed to provide genomic insights into its adaptation to milk and as a cell factory for EPS production during milk fermentation. The elucidation of ST 1275 genome makes this organism as a model dairy starter bacterium for the research of high EPS yield and capsular/ropy EPS producer from the species of S. thermophilus.-
dc.languageengen_US
dc.publisherAmerican Society of Animal Science. The Journal's web site is located at http://jas.fass.org-
dc.relation.ispartofJournal of Animal Scienceen_US
dc.rightsJournal of Animal Science. Copyright © American Society of Animal Science.-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectgenome sequencing-
dc.subjectEPS biosynthesis-
dc.subjectStreptococcus thermophilus-
dc.titleGenomic insights into high exopolysaccharideproducing dairy starter bacterium Streptococcus thermophilus ASCC 1275en_US
dc.typeConference_Paperen_US
dc.identifier.emailShah, N: npshah@hku.hken_US
dc.identifier.authorityShah, N=rp01571en_US
dc.description.naturepublished_or_final_version-
dc.identifier.hkuros240541en_US
dc.identifier.volume92-
dc.identifier.issueE-Suppl. 2-
dc.identifier.spage164, abstract no. 0332-
dc.identifier.epage164, abstract no. 0332-
dc.publisher.placeUnited States-

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