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Conference Paper: Anti-Inflammatory Effect of miR-17-3P VIA NF-κB Pathway in Human Endothelial Cells

TitleAnti-Inflammatory Effect of miR-17-3P VIA NF-κB Pathway in Human Endothelial Cells
Authors
Issue Date2013
PublisherMedcom Limited. The Journal's web site is located at http://www.hkcchk.com/journals.php
Citation
The 17th Annual Scientific Meeting of the Institute of Cardiovascular Science and Medicine (ICSM), The University of Hong Kong, Hong Kong, China, 23 November 2013. In Journal of the Hong Kong College of Cardiology, 2013, v. 21 n. 2, p. 71, abstract no. CP8 How to Cite?
AbstractObjective: MicroRNAs (miRNAs) are a class of small, noncoding RNAs of ≈22 nucleotides that negatively regulate gene expression. They appear to play a role in the development/progression of many disorders, including inflammation, cardiovascular diseases and endothelial dysfunction. This study examines the role of miR-17-3p in vascular inflammation. Methods: Human umbilical vein endothelial cells (HUVECs) were transfected with miR-17-3p agomir (miR-17-3p mimic) or its negative control using lipofectamine 2000. They were incubated with lipopolysaccharide (LPS, 10 ng/ml) for 16 hours to induce inflammatory reactions. The level of miR- 17-3p and the expressions of IκBα, p65 and phosphorylated p65 (p-p65) were measured by qPCR and Western blotting. The amount of interleukin-8 (IL-8) and tumor necrosis factor (TNF)-α released in the culture medium was detected with ELISA kit. Results: The levels of miR-17-3p, IL-8, TNF-α and p-p65 were increased following LPS stimulation. While the expression of p65 was not changed, the expression of IκBα was reduced. LPS-induced increase in IL-8 level and decrease in IκBα expression were smaller in HUVECs transfected with miR- 17-3p agomir than in those transfected with the negative control.
DescriptionConference Theme: Translating Advances in Science into Improvements in Cardiovascular Health
Chaired Poster Presentation
Persistent Identifierhttp://hdl.handle.net/10722/204447
ISSN
2015 SCImago Journal Rankings: 0.102

 

DC FieldValueLanguage
dc.contributor.authorZhang, Yen_US
dc.contributor.authorLeung, SWSen_US
dc.contributor.authorMan, RYKen_US
dc.date.accessioned2014-09-19T23:52:21Z-
dc.date.available2014-09-19T23:52:21Z-
dc.date.issued2013en_US
dc.identifier.citationThe 17th Annual Scientific Meeting of the Institute of Cardiovascular Science and Medicine (ICSM), The University of Hong Kong, Hong Kong, China, 23 November 2013. In Journal of the Hong Kong College of Cardiology, 2013, v. 21 n. 2, p. 71, abstract no. CP8en_US
dc.identifier.issn1027-7811-
dc.identifier.urihttp://hdl.handle.net/10722/204447-
dc.descriptionConference Theme: Translating Advances in Science into Improvements in Cardiovascular Health-
dc.descriptionChaired Poster Presentation-
dc.description.abstractObjective: MicroRNAs (miRNAs) are a class of small, noncoding RNAs of ≈22 nucleotides that negatively regulate gene expression. They appear to play a role in the development/progression of many disorders, including inflammation, cardiovascular diseases and endothelial dysfunction. This study examines the role of miR-17-3p in vascular inflammation. Methods: Human umbilical vein endothelial cells (HUVECs) were transfected with miR-17-3p agomir (miR-17-3p mimic) or its negative control using lipofectamine 2000. They were incubated with lipopolysaccharide (LPS, 10 ng/ml) for 16 hours to induce inflammatory reactions. The level of miR- 17-3p and the expressions of IκBα, p65 and phosphorylated p65 (p-p65) were measured by qPCR and Western blotting. The amount of interleukin-8 (IL-8) and tumor necrosis factor (TNF)-α released in the culture medium was detected with ELISA kit. Results: The levels of miR-17-3p, IL-8, TNF-α and p-p65 were increased following LPS stimulation. While the expression of p65 was not changed, the expression of IκBα was reduced. LPS-induced increase in IL-8 level and decrease in IκBα expression were smaller in HUVECs transfected with miR- 17-3p agomir than in those transfected with the negative control.-
dc.languageengen_US
dc.publisherMedcom Limited. The Journal's web site is located at http://www.hkcchk.com/journals.php-
dc.relation.ispartofJournal of the Hong Kong College of Cardiologyen_US
dc.titleAnti-Inflammatory Effect of miR-17-3P VIA NF-κB Pathway in Human Endothelial Cellsen_US
dc.typeConference_Paperen_US
dc.identifier.emailLeung, SWS: swsleung@hku.hken_US
dc.identifier.emailMan, RYK: rykman@hkucc.hku.hken_US
dc.identifier.authorityLeung, SWS=rp00235en_US
dc.identifier.authorityMan, RYK=rp00236en_US
dc.identifier.hkuros238802en_US
dc.identifier.volume21-
dc.identifier.issue2-
dc.identifier.spage71-
dc.identifier.epage71-
dc.publisher.placeHong Kong-

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