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Article: Endothelium-dependent and -independent relaxation of rat aorta induced by extract of Schizophyllum commune

TitleEndothelium-dependent and -independent relaxation of rat aorta induced by extract of Schizophyllum commune
Authors
Issue Date2014
PublisherElsevier GmbH - Urban und Fischer Verlag. The Journal's web site is located at http://www.elsevier.com/locate/phytomed
Citation
Phytomedicine, 2014, v. 21 n. 11, p. 1230-1236 How to Cite?
AbstractSchizophyllum commune (SC) is widely consumed by Chinese, especially in southern part of China. The aim of the present study was to assess the extract of SC on vascular tone and the mechanisms involved. Experiments were performed on aorta of 18-week-old male Sprague-Dawley rats. Dried SC was extracted with 50% ethanol, 90% ethanol and deionized water, respectively. The effects of SC on the isometric tension of rat aortic rings were measured. Protein expression for the endothelial nitric oxide synthase (eNOS) was also determined in the primarily cultured rat aortic arterial endothelial cells (RAECs). The results showed that the water extract of SC induced a marked relaxation in aortic rings with or without endothelium. After the pretreatments of Nω-nitro-l-arginine methyl ester, indomethacin, RP-cAMP, and methylene blue, the SC-induced relaxation was significantly decreased. In addition, the contraction due to Ca2+ influx and intracellular Ca2+ release was also inhibited by SC. Furthermore, expression of the eNOS protein was significantly elevated in RAECs after treatment of SC. In conclusion, the water extract of SC induces an endothelium-dependent and -independent relaxation in rat aorta. The relaxing effect of SC involves the modulation of NO-cGMP-dependent pathways, PGI2-cAMP-depedent pathways, Ca2+ influx though calcium channels and intracellular Ca2+ release.
Persistent Identifierhttp://hdl.handle.net/10722/203302
ISSN
2015 Impact Factor: 2.937
2015 SCImago Journal Rankings: 1.013
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChen, H-
dc.contributor.authorLi, S-
dc.contributor.authorWang, P-
dc.contributor.authorYan, S-
dc.contributor.authorHu, L-
dc.contributor.authorPan, X-
dc.contributor.authorYang, C-
dc.contributor.authorLeung, GPH-
dc.date.accessioned2014-09-19T13:56:57Z-
dc.date.available2014-09-19T13:56:57Z-
dc.date.issued2014-
dc.identifier.citationPhytomedicine, 2014, v. 21 n. 11, p. 1230-1236-
dc.identifier.issn0944-7113-
dc.identifier.urihttp://hdl.handle.net/10722/203302-
dc.description.abstractSchizophyllum commune (SC) is widely consumed by Chinese, especially in southern part of China. The aim of the present study was to assess the extract of SC on vascular tone and the mechanisms involved. Experiments were performed on aorta of 18-week-old male Sprague-Dawley rats. Dried SC was extracted with 50% ethanol, 90% ethanol and deionized water, respectively. The effects of SC on the isometric tension of rat aortic rings were measured. Protein expression for the endothelial nitric oxide synthase (eNOS) was also determined in the primarily cultured rat aortic arterial endothelial cells (RAECs). The results showed that the water extract of SC induced a marked relaxation in aortic rings with or without endothelium. After the pretreatments of Nω-nitro-l-arginine methyl ester, indomethacin, RP-cAMP, and methylene blue, the SC-induced relaxation was significantly decreased. In addition, the contraction due to Ca2+ influx and intracellular Ca2+ release was also inhibited by SC. Furthermore, expression of the eNOS protein was significantly elevated in RAECs after treatment of SC. In conclusion, the water extract of SC induces an endothelium-dependent and -independent relaxation in rat aorta. The relaxing effect of SC involves the modulation of NO-cGMP-dependent pathways, PGI2-cAMP-depedent pathways, Ca2+ influx though calcium channels and intracellular Ca2+ release.-
dc.languageeng-
dc.publisherElsevier GmbH - Urban und Fischer Verlag. The Journal's web site is located at http://www.elsevier.com/locate/phytomed-
dc.relation.ispartofPhytomedicine-
dc.titleEndothelium-dependent and -independent relaxation of rat aorta induced by extract of Schizophyllum commune-
dc.typeArticle-
dc.identifier.emailLeung, GPH: gphleung@hkucc.hku.hk-
dc.identifier.authorityLeung, GPH=rp00234en_US
dc.identifier.doi10.1016/j.phymed.2014.06.008-
dc.identifier.pmid25172784-
dc.identifier.hkuros240486-
dc.identifier.hkuros234174-
dc.identifier.volume21-
dc.identifier.issue11-
dc.identifier.spage1230-
dc.identifier.epage1236-
dc.identifier.isiWOS:000341741900002-
dc.publisher.placeGermany-

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