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Article: FasL expression on human nucleus pulposus cells contributes to the immune privilege of intervertebral disc by interacting with immunocytes

TitleFasL expression on human nucleus pulposus cells contributes to the immune privilege of intervertebral disc by interacting with immunocytes
Authors
Issue Date2013
PublisherIvyspring International Publisher. The Journal's web site is located at http://www.medsci.org/
Citation
Int J Med Sci, 2013, v. 10 n. 8, p. 1053-1060 How to Cite?
AbstractThe mechanisms of immune privilege in human nucleus pulposus (NP) remain unclear. Accumulating evidence indicates that Fas ligand (FasL) might play an important role in the immune privilege of the disc. We aimed for addressing the role of FasL expression in human intervertebral disc degeneration (IDD) and immune privilege in terms of the interaction between NP cells and immunocytes via the FasL-Fas machinery. We collected NP specimens from 20 patients with IDD as degenerative group and 8 normal cadaveric donors as control. FasL expression was detected by qRT-PCR, western blotting and flow cytometry (FCM). We also collected macrophages and CD8(+) T cells from the peripheral blood of patients with IDD for co-cultures with NP cells. And macrophages and CD8(+) T cells were harvested for apoptosis analysis by FCM after 2 days of co-cultures. We found that FasL expression in mRNA, protein and cellular resolutions demonstrated a significant decrease in degenerative group compared with normal control (p<0.05). FCM analysis found that human NP cells with increased FasL expression resulted in significantly increased apoptosis ratio of macrophages and CD8(+) T cells. Our study demonstrated that FasL expression tends to decrease in degenerated discs and FasL plays an important role in human disc immune privilege, which might provide a novel target for the treatment strategies for IDD.
Persistent Identifierhttp://hdl.handle.net/10722/203241
ISSN
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLiu, ZHen_US
dc.contributor.authorSun, Zen_US
dc.contributor.authorWang, HQen_US
dc.contributor.authorGe, Jen_US
dc.contributor.authorJiang, TSen_US
dc.contributor.authorChen, YFen_US
dc.contributor.authorMa, Yen_US
dc.contributor.authorWang, Cen_US
dc.contributor.authorHu, Sen_US
dc.contributor.authorSamartzis, Den_US
dc.contributor.authorLuo, ZJen_US
dc.date.accessioned2014-09-19T13:11:29Z-
dc.date.available2014-09-19T13:11:29Z-
dc.date.issued2013en_US
dc.identifier.citationInt J Med Sci, 2013, v. 10 n. 8, p. 1053-1060en_US
dc.identifier.issn1449-1907 (Electronic) 1449-1907 (Linkinen_US
dc.identifier.urihttp://hdl.handle.net/10722/203241-
dc.description.abstractThe mechanisms of immune privilege in human nucleus pulposus (NP) remain unclear. Accumulating evidence indicates that Fas ligand (FasL) might play an important role in the immune privilege of the disc. We aimed for addressing the role of FasL expression in human intervertebral disc degeneration (IDD) and immune privilege in terms of the interaction between NP cells and immunocytes via the FasL-Fas machinery. We collected NP specimens from 20 patients with IDD as degenerative group and 8 normal cadaveric donors as control. FasL expression was detected by qRT-PCR, western blotting and flow cytometry (FCM). We also collected macrophages and CD8(+) T cells from the peripheral blood of patients with IDD for co-cultures with NP cells. And macrophages and CD8(+) T cells were harvested for apoptosis analysis by FCM after 2 days of co-cultures. We found that FasL expression in mRNA, protein and cellular resolutions demonstrated a significant decrease in degenerative group compared with normal control (p<0.05). FCM analysis found that human NP cells with increased FasL expression resulted in significantly increased apoptosis ratio of macrophages and CD8(+) T cells. Our study demonstrated that FasL expression tends to decrease in degenerated discs and FasL plays an important role in human disc immune privilege, which might provide a novel target for the treatment strategies for IDD.en_US
dc.languageengen_US
dc.publisherIvyspring International Publisher. The Journal's web site is located at http://www.medsci.org/-
dc.relation.ispartofInt J Med Scien_US
dc.rightsInternational journal of medical sciences. Copyright © Ivyspring International Publisher-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titleFasL expression on human nucleus pulposus cells contributes to the immune privilege of intervertebral disc by interacting with immunocytesen_US
dc.typeArticleen_US
dc.identifier.emailSamartzis, D: dspine@hku.hken_US
dc.identifier.authoritySamartzis, D=rp01430en_US
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.7150/ijms.6223en_US
dc.identifier.pmid23801893-
dc.identifier.pmcidPMC3691805-
dc.identifier.hkuros237996en_US
dc.identifier.volume10en_US
dc.identifier.issue8en_US
dc.identifier.spage1053en_US
dc.identifier.epage1060en_US
dc.identifier.isiWOS:000321514900015-

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