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Article: Insights into the hallmarks of human nucleus pulposus cells with particular reference to cell viability, phagocytic potential and long process formation

TitleInsights into the hallmarks of human nucleus pulposus cells with particular reference to cell viability, phagocytic potential and long process formation
Authors
Issue Date2013
PublisherIvyspring International Publisher. The Journal's web site is located at http://www.medsci.org/
Citation
Int J Med Sci, 2013, v. 10 n. 13, p. 1805-1816 How to Cite?
AbstractOBJECTIVE: As a main cellular component within the disc, nucleus pulposus (NP) cells play important roles in disc physiology. However, little is known on the biologic hallmarks of human NP cells. Therefore, the present study aimed to address the features of human NP cells. METHODS: Human NP samples were collected from normal cadavers, patients with scoliosis and disc degeneration as normal, disease control and degenerative NP, respectively. The NP samples were studied using transmission electron microscopy and TUNEL assay. Pre-digested NP samples were studied using flow cytometry with PI/Annexin V staining. RESULTS: Both control and degenerative human NP consisted of mainly viable cells with a variety of morphology. Both necrosis and apoptosis were noted in human NP as forms of cell death with increased apoptosis in degenerative NP, which was further confirmed by the TUNEL assay. Phagocytic NP cells had the hallmarks of both stationary macrophages with lysosomes and NP cells with the endoplasmic reticulum. Annulus fibrosus cells have similar morphologic characteristics with NP cells in terms of cell nest, phagocytosis and intracellular organs. Moreover, NP cells with long processes existed in degenerative and scoliotic NP rather than normal NP. When cultured in glucose-free medium, NP cells developed long and thin processes. CONCLUSION: Human degenerative NP consists of primarily viable cells. We present direct and in vivo evidence that both human annulus fibrosus and NP cells have phagocytic potential. Moreover, NP cells with long processes exist in both scoliotic and degenerative NP with lack of glucose as one of the possible underlying mechanisms.
Persistent Identifierhttp://hdl.handle.net/10722/203240
ISSN
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChen, YFen_US
dc.contributor.authorZhang, YZen_US
dc.contributor.authorZhang, WLen_US
dc.contributor.authorLuan, GNen_US
dc.contributor.authorLiu, ZHen_US
dc.contributor.authorGao, Yen_US
dc.contributor.authorWan, ZYen_US
dc.contributor.authorSun, Zen_US
dc.contributor.authorZhu, Sen_US
dc.contributor.authorSamartzis, Den_US
dc.contributor.authorWang, CMen_US
dc.contributor.authorWang, HQen_US
dc.contributor.authorLuo, ZJen_US
dc.date.accessioned2014-09-19T13:11:25Z-
dc.date.available2014-09-19T13:11:25Z-
dc.date.issued2013en_US
dc.identifier.citationInt J Med Sci, 2013, v. 10 n. 13, p. 1805-1816en_US
dc.identifier.issn1449-1907 (Electronic) 1449-1907 (Linkinen_US
dc.identifier.urihttp://hdl.handle.net/10722/203240-
dc.description.abstractOBJECTIVE: As a main cellular component within the disc, nucleus pulposus (NP) cells play important roles in disc physiology. However, little is known on the biologic hallmarks of human NP cells. Therefore, the present study aimed to address the features of human NP cells. METHODS: Human NP samples were collected from normal cadavers, patients with scoliosis and disc degeneration as normal, disease control and degenerative NP, respectively. The NP samples were studied using transmission electron microscopy and TUNEL assay. Pre-digested NP samples were studied using flow cytometry with PI/Annexin V staining. RESULTS: Both control and degenerative human NP consisted of mainly viable cells with a variety of morphology. Both necrosis and apoptosis were noted in human NP as forms of cell death with increased apoptosis in degenerative NP, which was further confirmed by the TUNEL assay. Phagocytic NP cells had the hallmarks of both stationary macrophages with lysosomes and NP cells with the endoplasmic reticulum. Annulus fibrosus cells have similar morphologic characteristics with NP cells in terms of cell nest, phagocytosis and intracellular organs. Moreover, NP cells with long processes existed in degenerative and scoliotic NP rather than normal NP. When cultured in glucose-free medium, NP cells developed long and thin processes. CONCLUSION: Human degenerative NP consists of primarily viable cells. We present direct and in vivo evidence that both human annulus fibrosus and NP cells have phagocytic potential. Moreover, NP cells with long processes exist in both scoliotic and degenerative NP with lack of glucose as one of the possible underlying mechanisms.en_US
dc.languageengen_US
dc.publisherIvyspring International Publisher. The Journal's web site is located at http://www.medsci.org/-
dc.relation.ispartofInt J Med Scien_US
dc.rightsInternational journal of medical sciences. Copyright © Ivyspring International Publisher-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titleInsights into the hallmarks of human nucleus pulposus cells with particular reference to cell viability, phagocytic potential and long process formationen_US
dc.typeArticleen_US
dc.identifier.emailSamartzis, D: dspine@hku.hken_US
dc.identifier.authoritySamartzis, D=rp01430en_US
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.7150/ijms.6530en_US
dc.identifier.pmid24324357-
dc.identifier.hkuros237995en_US
dc.identifier.volume10en_US
dc.identifier.issue13en_US
dc.identifier.spage1805en_US
dc.identifier.epage1816en_US
dc.identifier.isiWOS:000328163400001-

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