File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Down-regulated CK8 expression in human intervertebral disc degeneration

TitleDown-regulated CK8 expression in human intervertebral disc degeneration
Authors
Issue Date2013
PublisherIvyspring International Publisher. The Journal's web site is located at http://www.medsci.org/
Citation
Int J Med Sci, 2013, v. 10 n. 8, p. 948-956 How to Cite?
AbstractAs an intermediate filament protein, cytokeratin 8 (CK8) exerts multiple cellular functions. Moreover, it has been identified as a marker of notochord cells, which play essential roles in human nucleus pulposus (NP). However, the distribution of CK8 positive cells in human NP and their relationship with intervertebral disc degeneration (IDD) have not been clarified until now. Here, we found the percentage of CK8 positive cells in IDD (25.7+/-4.14%) was significantly lower than that in normal and scoliosis NP (51.9+/-9.73% and 47.8+/-5.51%, respectively, p<0.05). Western blotting and qRT-PCR results confirmed the down-regulation of CK8 expression in IDD on both of protein and mRNA levels. Furthermore, approximately 37.4% of cell clusters were CK8 positive in IDD. Taken together, this is the first study to show a down-regulated CK8 expression and the percentage of CK8 positive cell clusters in IDD based upon multiple lines of evidence. Consequently, CK8 positive cells might be considered as a potential option in the development of cellular treatment strategies for NP repair.
Persistent Identifierhttp://hdl.handle.net/10722/203239
ISSN
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorSun, Zen_US
dc.contributor.authorWang, HQen_US
dc.contributor.authorLiu, ZHen_US
dc.contributor.authorChang, Len_US
dc.contributor.authorChen, YFen_US
dc.contributor.authorZhang, YZen_US
dc.contributor.authorZhang, WLen_US
dc.contributor.authorGao, Yen_US
dc.contributor.authorWan, ZYen_US
dc.contributor.authorChe, Len_US
dc.contributor.authorLiu, Xen_US
dc.contributor.authorSamartzis, Den_US
dc.contributor.authorLuo, ZJen_US
dc.date.accessioned2014-09-19T13:11:20Z-
dc.date.available2014-09-19T13:11:20Z-
dc.date.issued2013en_US
dc.identifier.citationInt J Med Sci, 2013, v. 10 n. 8, p. 948-956en_US
dc.identifier.issn1449-1907 (Electronic) 1449-1907 (Linkinen_US
dc.identifier.urihttp://hdl.handle.net/10722/203239-
dc.description.abstractAs an intermediate filament protein, cytokeratin 8 (CK8) exerts multiple cellular functions. Moreover, it has been identified as a marker of notochord cells, which play essential roles in human nucleus pulposus (NP). However, the distribution of CK8 positive cells in human NP and their relationship with intervertebral disc degeneration (IDD) have not been clarified until now. Here, we found the percentage of CK8 positive cells in IDD (25.7+/-4.14%) was significantly lower than that in normal and scoliosis NP (51.9+/-9.73% and 47.8+/-5.51%, respectively, p<0.05). Western blotting and qRT-PCR results confirmed the down-regulation of CK8 expression in IDD on both of protein and mRNA levels. Furthermore, approximately 37.4% of cell clusters were CK8 positive in IDD. Taken together, this is the first study to show a down-regulated CK8 expression and the percentage of CK8 positive cell clusters in IDD based upon multiple lines of evidence. Consequently, CK8 positive cells might be considered as a potential option in the development of cellular treatment strategies for NP repair.en_US
dc.languageengen_US
dc.publisherIvyspring International Publisher. The Journal's web site is located at http://www.medsci.org/-
dc.relation.ispartofInt J Med Scien_US
dc.rightsInternational journal of medical sciences. Copyright © Ivyspring International Publisher-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titleDown-regulated CK8 expression in human intervertebral disc degenerationen_US
dc.typeArticleen_US
dc.identifier.emailSamartzis, D: dspine@hku.hken_US
dc.identifier.authoritySamartzis, D=rp01430en_US
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.7150/ijms.5642en_US
dc.identifier.pmid23801880-
dc.identifier.pmcidPMC3691792-
dc.identifier.hkuros237994en_US
dc.identifier.volume10en_US
dc.identifier.issue8en_US
dc.identifier.spage948en_US
dc.identifier.epage956en_US
dc.identifier.isiWOS:000321514900002-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats