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Article: Evaluation of real-time PCR for quantitative detection of Escherichia coli in beach water

TitleEvaluation of real-time PCR for quantitative detection of Escherichia coli in beach water
Authors
Issue Date2014
Citation
J Water Health, 2014, v. 12 n. 1, p. 51-6 How to Cite?
AbstractThe current investigation evaluated the use of real-time polymerase chain reaction (PCR) for quantitative detection of Escherichia coli in marine beach water. Densities of E. coli in 263 beach water samples collected from 13 bathing beaches in Hong Kong between November 2008 and December 2009 were determined using both real-time PCR and culture-based methods. Regression analysis showed that these two methods had a significant positive linear relationship with a correlation coefficient (r) of 0.64. Serial dilution of spiked samples indicated that the real-time PCR had a limit of quantification of 25 E. coli colonies in 100 mL water sample. This study showed that the rapid real-time PCR has potential to complement the traditional culture method of assessing fecal pollution in marine beach water.
Persistent Identifierhttp://hdl.handle.net/10722/203188
ISSN
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLam, THen_US
dc.contributor.authorLui, Een_US
dc.contributor.authorChau, Sen_US
dc.contributor.authorKueh, CSen_US
dc.contributor.authorYung, YKen_US
dc.contributor.authorYam, WCen_US
dc.date.accessioned2014-09-19T12:56:57Z-
dc.date.available2014-09-19T12:56:57Z-
dc.date.issued2014en_US
dc.identifier.citationJ Water Health, 2014, v. 12 n. 1, p. 51-6en_US
dc.identifier.issn1477-8920 (Print) 1477-8920 (Linking)en_US
dc.identifier.urihttp://hdl.handle.net/10722/203188-
dc.description.abstractThe current investigation evaluated the use of real-time polymerase chain reaction (PCR) for quantitative detection of Escherichia coli in marine beach water. Densities of E. coli in 263 beach water samples collected from 13 bathing beaches in Hong Kong between November 2008 and December 2009 were determined using both real-time PCR and culture-based methods. Regression analysis showed that these two methods had a significant positive linear relationship with a correlation coefficient (r) of 0.64. Serial dilution of spiked samples indicated that the real-time PCR had a limit of quantification of 25 E. coli colonies in 100 mL water sample. This study showed that the rapid real-time PCR has potential to complement the traditional culture method of assessing fecal pollution in marine beach water.en_US
dc.languageengen_US
dc.relation.ispartofJ Water Healthen_US
dc.titleEvaluation of real-time PCR for quantitative detection of Escherichia coli in beach wateren_US
dc.typeArticleen_US
dc.identifier.emailLam, TH: jasonlam@graduate.hku.hken_US
dc.identifier.emailYam, WC: wcyam@hkucc.hku.hken_US
dc.identifier.authorityYam, WC=rp00313en_US
dc.identifier.doi10.2166/wh.2013.038en_US
dc.identifier.pmid24642432-
dc.identifier.hkuros240440en_US
dc.identifier.volume12en_US
dc.identifier.issue1en_US
dc.identifier.spage51en_US
dc.identifier.epage6en_US
dc.identifier.isiWOS:000338511500006-

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