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Article: Detection of extracellular matrix degradation in intervertebral disc degeneration by diffusion magnetic resonance spectroscopy

TitleDetection of extracellular matrix degradation in intervertebral disc degeneration by diffusion magnetic resonance spectroscopy
Authors
KeywordsDiffusion weighted MRS
Extracellular matrix
Intervertebral disc degeneration
Magnetic resonance spectroscopy
Proteoglycan
Issue Date2015
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0740-3194/
Citation
Magnetic Resonance in Medicine, 2015, v. 73 n. 5, p. 1703-1712 How to Cite?
AbstractPurpose To investigate whether diffusion magnetic resonance spectroscopy (MRS) can detect the extracellular matrix (ECM) degradation during intervertebral disc degeneration (IVDD) by the increased mobility of ECM macromolecules such as proteoglycans and collagens. Methods Fresh bovine intervertebral discs were injected with papain solution to induce ECM degradation. The apparent diffusion coefficients (ADCs), T2 values, and contents of ECM macromolecules and water resonances were measured longitudinally in the nucleus pulposus. Results The macromolecule ADCs increased drastically at day 1 after papain injection, and continued increasing for 5 days. In contrast, the proteoglycan content exhibited a small and slow decrease after injection while the macromolecule T2 values, water T2, ADC, and content showed slight increase or no change. The protein gel electrophoresis analysis confirmed the gradually increased ECM fragmentation in accordance with the observed macromolecule ADC increases. Conclusion Diffusion MRS provides a new method to characterize the ECM degradation processes directly and sensitively. Macromolecule ADCs offer a potentially more sensitive and earlier marker for ECM degradation than the proteoglycan content and T2, and water MR properties during early IVDD. Such diffusion approach offers the possibility to directly monitor ECM integrity and degradation processes in vivo at molecular and microstructural levels in both preclinical and clinical settings. Magn Reson Med 73:1703-1712, 2015.
Persistent Identifierhttp://hdl.handle.net/10722/202531
ISSN
2017 Impact Factor: 4.082
2015 SCImago Journal Rankings: 2.197
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWang, AM-
dc.contributor.authorCao, P-
dc.contributor.authorYee, FYA-
dc.contributor.authorChan, D-
dc.contributor.authorWu, EX-
dc.date.accessioned2014-09-19T08:25:14Z-
dc.date.available2014-09-19T08:25:14Z-
dc.date.issued2015-
dc.identifier.citationMagnetic Resonance in Medicine, 2015, v. 73 n. 5, p. 1703-1712-
dc.identifier.issn0740-3194-
dc.identifier.urihttp://hdl.handle.net/10722/202531-
dc.description.abstractPurpose To investigate whether diffusion magnetic resonance spectroscopy (MRS) can detect the extracellular matrix (ECM) degradation during intervertebral disc degeneration (IVDD) by the increased mobility of ECM macromolecules such as proteoglycans and collagens. Methods Fresh bovine intervertebral discs were injected with papain solution to induce ECM degradation. The apparent diffusion coefficients (ADCs), T2 values, and contents of ECM macromolecules and water resonances were measured longitudinally in the nucleus pulposus. Results The macromolecule ADCs increased drastically at day 1 after papain injection, and continued increasing for 5 days. In contrast, the proteoglycan content exhibited a small and slow decrease after injection while the macromolecule T2 values, water T2, ADC, and content showed slight increase or no change. The protein gel electrophoresis analysis confirmed the gradually increased ECM fragmentation in accordance with the observed macromolecule ADC increases. Conclusion Diffusion MRS provides a new method to characterize the ECM degradation processes directly and sensitively. Macromolecule ADCs offer a potentially more sensitive and earlier marker for ECM degradation than the proteoglycan content and T2, and water MR properties during early IVDD. Such diffusion approach offers the possibility to directly monitor ECM integrity and degradation processes in vivo at molecular and microstructural levels in both preclinical and clinical settings. Magn Reson Med 73:1703-1712, 2015.-
dc.languageeng-
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0740-3194/-
dc.relation.ispartofMagnetic Resonance in Medicine-
dc.subjectDiffusion weighted MRS-
dc.subjectExtracellular matrix-
dc.subjectIntervertebral disc degeneration-
dc.subjectMagnetic resonance spectroscopy-
dc.subjectProteoglycan-
dc.titleDetection of extracellular matrix degradation in intervertebral disc degeneration by diffusion magnetic resonance spectroscopy-
dc.typeArticle-
dc.identifier.emailCao, P: caopeng1@hku.hk-
dc.identifier.emailYee, FYA: fyayee@hku.hk-
dc.identifier.emailChan, D: chand@hku.hk-
dc.identifier.emailWu, EX: ewu1@hkucc.hku.hk-
dc.identifier.authorityChan, D=rp00540-
dc.identifier.authorityWu, EX=rp00193-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/mrm.25289-
dc.identifier.pmid24817583-
dc.identifier.scopuseid_2-s2.0-84928012350-
dc.identifier.hkuros240278-
dc.identifier.hkuros255269-
dc.identifier.volume73-
dc.identifier.issue5-
dc.identifier.spage1703-
dc.identifier.epage1712-
dc.identifier.isiWOS:000353240600001-
dc.publisher.placeUnited States-

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