File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Response of the Hepatic Transcriptome to Aflatoxin B1 in Domestic Turkey (Meleagris gallopavo)

TitleResponse of the Hepatic Transcriptome to Aflatoxin B1 in Domestic Turkey (Meleagris gallopavo)
Authors
Issue Date2014
PublisherPLOS. The Journal's web site is located at http://www.plosone.org/home.action
Citation
PLoS One, 2014, v. 9 n. 6, p. e100930 How to Cite?
AbstractDietary exposure to aflatoxin B1 (AFB1) is detrimental to avian health and leads to major economic losses for the poultry industry. AFB1 is especially hepatotoxic in domestic turkeys (Meleagris gallopavo), since these birds are unable to detoxify AFB1 by glutathione-conjugation. The impacts of AFB1 on the turkey hepatic transcriptome and the potential protection from pretreatment with a Lactobacillus-based probiotic mixture were investigated through RNA-sequencing. Animals were divided into four treatment groups and RNA was subsequently recovered from liver samples. Four pooled RNA-seq libraries were sequenced to produce over 322 M reads totaling 13.8 Gb of sequence. Approximately 170,000 predicted transcripts were de novo assembled, of which 803 had significant differential expression in at least one pair-wise comparison between treatment groups. Functional analysis linked many of the transcripts significantly affected by AFB1 exposure to cancer, apoptosis, the cell cycle or lipid regulation. Most notable were transcripts from the genes encoding E3 ubiquitin-protein ligase Mdm2, osteopontin, S-adenosylmethionine synthase isoform type-2, and lipoprotein lipase. Expression was modulated by the probiotics, but treatment did not completely mitigate the effects of AFB1. Genes identified through transcriptome analysis provide candidates for further study of AFB1 toxicity and targets for efforts to improve the health of domestic turkeys exposed to AFB1.
Persistent Identifierhttp://hdl.handle.net/10722/201544
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorMonson, MSen_US
dc.contributor.authorSettlage, REen_US
dc.contributor.authorMcMahon, KWen_US
dc.contributor.authorMendoza, KMen_US
dc.contributor.authorRawal, Sen_US
dc.contributor.authorEl-Nezamy, HSen_US
dc.contributor.authorCoulombe, RAen_US
dc.contributor.authorReed, KMen_US
dc.date.accessioned2014-08-21T07:30:23Z-
dc.date.available2014-08-21T07:30:23Z-
dc.date.issued2014en_US
dc.identifier.citationPLoS One, 2014, v. 9 n. 6, p. e100930en_US
dc.identifier.urihttp://hdl.handle.net/10722/201544-
dc.description.abstractDietary exposure to aflatoxin B1 (AFB1) is detrimental to avian health and leads to major economic losses for the poultry industry. AFB1 is especially hepatotoxic in domestic turkeys (Meleagris gallopavo), since these birds are unable to detoxify AFB1 by glutathione-conjugation. The impacts of AFB1 on the turkey hepatic transcriptome and the potential protection from pretreatment with a Lactobacillus-based probiotic mixture were investigated through RNA-sequencing. Animals were divided into four treatment groups and RNA was subsequently recovered from liver samples. Four pooled RNA-seq libraries were sequenced to produce over 322 M reads totaling 13.8 Gb of sequence. Approximately 170,000 predicted transcripts were de novo assembled, of which 803 had significant differential expression in at least one pair-wise comparison between treatment groups. Functional analysis linked many of the transcripts significantly affected by AFB1 exposure to cancer, apoptosis, the cell cycle or lipid regulation. Most notable were transcripts from the genes encoding E3 ubiquitin-protein ligase Mdm2, osteopontin, S-adenosylmethionine synthase isoform type-2, and lipoprotein lipase. Expression was modulated by the probiotics, but treatment did not completely mitigate the effects of AFB1. Genes identified through transcriptome analysis provide candidates for further study of AFB1 toxicity and targets for efforts to improve the health of domestic turkeys exposed to AFB1.en_US
dc.languageengen_US
dc.publisherPLOS. The Journal's web site is located at http://www.plosone.org/home.actionen_US
dc.relation.ispartofPLoS Oneen_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titleResponse of the Hepatic Transcriptome to Aflatoxin B1 in Domestic Turkey (Meleagris gallopavo)en_US
dc.typeArticleen_US
dc.identifier.emailEl-Nezamy, HS: elnezami@hkucc.hku.hken_US
dc.identifier.authorityEl-Nezamy, HS=rp00694en_US
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1371/journal.pone.0100930en_US
dc.identifier.pmid24979717-
dc.identifier.hkuros232330en_US
dc.identifier.volume9en_US
dc.identifier.isiWOS:000338506400051-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats