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Conference Paper: The Effect of Astragalus Membranaceus on Pathological Angiogenesis

TitleThe Effect of Astragalus Membranaceus on Pathological Angiogenesis
Authors
Issue Date2013
PublisherMedcom Limited. The Journal's web site is located at http://www.hkcchk.com/journals.php
Citation
The 17th Annual Scientific Meeting of the Institute of Cardiovascular Science and Medicine (ICSM), The University of Hong Kong, Hong Kong, China, 23 November 2013. In Journal of the Hong Kong College of Cardiology, 2013, v. 21 n. 2, p. 75, abstract no. P07 How to Cite?
AbstractAbnormality of angiogenesis can contribute to several pathologic processes including atherosclerosis and cancer. Different bioactive components of astragalus membranaceus (huangqi), a commonly used herb in traditional Chinese medicine, exert different effects on the angiogenesis of human cancer cells and endothelial cells. Therefore, the effect of astragalus membranaceus and its active components on angiogenesis under different pathological conditions were investigated. Human umbilical endothelial cells (HUVECs) and human colon cancer cell HCT 116 were used for in vitro studies. The effect of astragalus membranaceus on proliferation were examined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The results indicated that the viabilities of HUVECs treated with astragalus saponin (0.1 to 25 µg/ml), astragaloside IV (0.01 to 100 µM) and calycosin (1 to 200 µM) for 24-72 hours were not significantly different from the control group (without any treatment). Astragalus saponin and astragaloside IV, at the same concentrations as used in HUVECs, also did not affect the viability of HCT 116 cells, while the highest concentration of calycosin (200 µM) inhibited the growth of HCT 116 cells. The angiogenic potential of these components of astragalus membranaceus are examined in both HUVECs [under the stimulation of phorbol myristate acetate (PMA), a protein kinase C activator] and HCT 116 cells by wound healing migration assay and tube formation assay, in order to identify the influence of astragalus membranaceus and its components on pathological angiogenesis.
DescriptionConference Theme: Translating Advances in Science into Improvements in Cardiovascular Health
Poster Presentation
Persistent Identifierhttp://hdl.handle.net/10722/201339
ISSN
2015 SCImago Journal Rankings: 0.102

 

DC FieldValueLanguage
dc.contributor.authorLiu, WYen_US
dc.contributor.authorAuyeung, KKWen_US
dc.contributor.authorKo, JKSen_US
dc.contributor.authorMan, RYKen_US
dc.contributor.authorLeung, SWSen_US
dc.date.accessioned2014-08-21T07:24:49Z-
dc.date.available2014-08-21T07:24:49Z-
dc.date.issued2013en_US
dc.identifier.citationThe 17th Annual Scientific Meeting of the Institute of Cardiovascular Science and Medicine (ICSM), The University of Hong Kong, Hong Kong, China, 23 November 2013. In Journal of the Hong Kong College of Cardiology, 2013, v. 21 n. 2, p. 75, abstract no. P07en_US
dc.identifier.issn1027-7811-
dc.identifier.urihttp://hdl.handle.net/10722/201339-
dc.descriptionConference Theme: Translating Advances in Science into Improvements in Cardiovascular Health-
dc.descriptionPoster Presentation-
dc.description.abstractAbnormality of angiogenesis can contribute to several pathologic processes including atherosclerosis and cancer. Different bioactive components of astragalus membranaceus (huangqi), a commonly used herb in traditional Chinese medicine, exert different effects on the angiogenesis of human cancer cells and endothelial cells. Therefore, the effect of astragalus membranaceus and its active components on angiogenesis under different pathological conditions were investigated. Human umbilical endothelial cells (HUVECs) and human colon cancer cell HCT 116 were used for in vitro studies. The effect of astragalus membranaceus on proliferation were examined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The results indicated that the viabilities of HUVECs treated with astragalus saponin (0.1 to 25 µg/ml), astragaloside IV (0.01 to 100 µM) and calycosin (1 to 200 µM) for 24-72 hours were not significantly different from the control group (without any treatment). Astragalus saponin and astragaloside IV, at the same concentrations as used in HUVECs, also did not affect the viability of HCT 116 cells, while the highest concentration of calycosin (200 µM) inhibited the growth of HCT 116 cells. The angiogenic potential of these components of astragalus membranaceus are examined in both HUVECs [under the stimulation of phorbol myristate acetate (PMA), a protein kinase C activator] and HCT 116 cells by wound healing migration assay and tube formation assay, in order to identify the influence of astragalus membranaceus and its components on pathological angiogenesis.-
dc.languageengen_US
dc.publisherMedcom Limited. The Journal's web site is located at http://www.hkcchk.com/journals.php-
dc.relation.ispartofJournal of the Hong Kong College of Cardiologyen_US
dc.titleThe Effect of Astragalus Membranaceus on Pathological Angiogenesisen_US
dc.typeConference_Paperen_US
dc.identifier.emailMan, RYK: rykman@hkucc.hku.hken_US
dc.identifier.emailLeung, SWS: swsleung@hku.hken_US
dc.identifier.authorityMan, RYK=rp00236en_US
dc.identifier.authorityLeung, SWS=rp00235en_US
dc.identifier.hkuros233045en_US
dc.identifier.volume21-
dc.identifier.issue2-
dc.identifier.spage75-
dc.identifier.epage75-
dc.publisher.placeHong Kong-

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