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Conference Paper: FBI-1 and choriocarcinoma cell proliferation

TitleFBI-1 and choriocarcinoma cell proliferation
Authors
Issue Date2013
Citation
The 20th Hong Kong International Cancer Congress (HKICC 2013), Hong Kong, 14-15 November 2013. How to Cite?
AbstractGestational trophoblastic disease (GTD) includes a spectrum of diseases that involve abnormal growth of trophoblastic cells inside the uterus. It can range from benign hydatidiform moles (HM) to frankly malignant choriocarcinoma, placental site trophoblastic tumor (PSTT) or epithelioid trophoblastic tumour (ETT). Most choriocarcinoma arise from HM but can develop from any pregnancy related events such as ectopic pregnancy, live-birth or stillbirth. Being the most aggressive neoplasm in GTD, choriocarcinoma can develop widespread metastasis and can be fatal. FBI-1 (Pokemon) is a transcriptional factor that is often overexpressed in various types of human cancer. We have reported overexpression of FBI-1 in ovarian cancer in association with cell proliferation and invasiveness. Our recent study also suggested that overexpression of FBI-1 in HM was related to subsequent development of gestational trophoblastic neoplasia (GTN). In this study, we evaluated the role of FBI-1 gene and choriocarcinoma cell proliferation. By MTT assay, the proliferation rates of two choriocarcinoma cell lines (JAR and JEG-3) was found to decrease when FBI-1 was downregulated by shRNA approach with statistical significance reached in JEG-3 (p < 0.05). By quantitative real time PCR, the relative levels of a panel of stem cell related genes, including Shh, Patched, Gli1, Gli2, Gli3, Kif7, Nanog, Oct4, Sox2, and Stat3, were assessed after knockdown of FBI-1 gene. Significant downregulation of Shh (Sonic Hedgehog) transcript in choriocarcinoma cells in association with reduced proliferation. In conclusion, FBI-1 may play a role in choriocarcinoma cell proliferation and FBI-1 may be explored as one potential therapeutic target for GTD in the future.
DescriptionTheme: New Horizons in Cancer Care
Persistent Identifierhttp://hdl.handle.net/10722/201316

 

DC FieldValueLanguage
dc.contributor.authorCheung, AMen_US
dc.contributor.authorWong, GWen_US
dc.contributor.authorChan, KKen_US
dc.contributor.authorWong, ESYen_US
dc.contributor.authorCheung, ANYen_US
dc.date.accessioned2014-08-21T07:22:57Z-
dc.date.available2014-08-21T07:22:57Z-
dc.date.issued2013en_US
dc.identifier.citationThe 20th Hong Kong International Cancer Congress (HKICC 2013), Hong Kong, 14-15 November 2013.en_US
dc.identifier.urihttp://hdl.handle.net/10722/201316-
dc.descriptionTheme: New Horizons in Cancer Care-
dc.description.abstractGestational trophoblastic disease (GTD) includes a spectrum of diseases that involve abnormal growth of trophoblastic cells inside the uterus. It can range from benign hydatidiform moles (HM) to frankly malignant choriocarcinoma, placental site trophoblastic tumor (PSTT) or epithelioid trophoblastic tumour (ETT). Most choriocarcinoma arise from HM but can develop from any pregnancy related events such as ectopic pregnancy, live-birth or stillbirth. Being the most aggressive neoplasm in GTD, choriocarcinoma can develop widespread metastasis and can be fatal. FBI-1 (Pokemon) is a transcriptional factor that is often overexpressed in various types of human cancer. We have reported overexpression of FBI-1 in ovarian cancer in association with cell proliferation and invasiveness. Our recent study also suggested that overexpression of FBI-1 in HM was related to subsequent development of gestational trophoblastic neoplasia (GTN). In this study, we evaluated the role of FBI-1 gene and choriocarcinoma cell proliferation. By MTT assay, the proliferation rates of two choriocarcinoma cell lines (JAR and JEG-3) was found to decrease when FBI-1 was downregulated by shRNA approach with statistical significance reached in JEG-3 (p < 0.05). By quantitative real time PCR, the relative levels of a panel of stem cell related genes, including Shh, Patched, Gli1, Gli2, Gli3, Kif7, Nanog, Oct4, Sox2, and Stat3, were assessed after knockdown of FBI-1 gene. Significant downregulation of Shh (Sonic Hedgehog) transcript in choriocarcinoma cells in association with reduced proliferation. In conclusion, FBI-1 may play a role in choriocarcinoma cell proliferation and FBI-1 may be explored as one potential therapeutic target for GTD in the future.en_US
dc.languageengen_US
dc.relation.ispartof20th Hong Kong International Cancer Congress, HKICC 2013en_US
dc.titleFBI-1 and choriocarcinoma cell proliferationen_US
dc.typeConference_Paperen_US
dc.identifier.emailWong, GW: wonggw@hkucc.hku.hken_US
dc.identifier.emailChan, KK: kuiasdf@hku.hken_US
dc.identifier.emailWong, ESY: esywong@hkucc.hku.hken_US
dc.identifier.emailCheung, ANY: anycheun@hkucc.hku.hken_US
dc.identifier.authorityCheung, ANY=rp00542en_US
dc.identifier.hkuros232392en_US

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