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Conference Paper: Revisit laser scanning fluorescence microscopy performance under fluorescence-lifetime-limited regime

TitleRevisit laser scanning fluorescence microscopy performance under fluorescence-lifetime-limited regime
Authors
KeywordsImage resolution
Laser scanning fluorescence microscopy
Signal-to-noise ratio
Speed limit
Issue Date2014
PublisherSPIE - International Society for Optical Engineering.
Citation
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII; San Francisco, CA., 3-6 February 2014. In Proceedings of SPIE, 2014, v. 8947, article no. 894726 How to Cite?
AbstractContinuing desire for higher-speed laser scanning fluorescence microscopy (LSFM) and progressive advancement in ultrafast and sensitive photodetectors might imply that our conventional understanding of LSFM is not adequate when approaching to the intrinsic speed limit - fluorescence lifetime. In this regard, we here revisit the theoretical framework of LSFM and evaluate its general performance in lifetime-limited and noise-limited regimes. Our model suggests that there still exists an order-of-magnitude gap between the current LSFM speed and the intrinsic limit. An imaging frame rate of > 100 kHz could be viable with the emerging laser-scanning techniques using ultrafast wavelength-swept sources, or optical time-stretch. © 2014 SPIE.
Persistent Identifierhttp://hdl.handle.net/10722/201229
ISBN
ISSN

 

DC FieldValueLanguage
dc.contributor.authorChan, ACSen_US
dc.contributor.authorWong, TTWen_US
dc.contributor.authorWong, KKYen_US
dc.contributor.authorLam, EYMen_US
dc.contributor.authorTsia, KKMen_US
dc.date.accessioned2014-08-21T07:18:17Z-
dc.date.available2014-08-21T07:18:17Z-
dc.date.issued2014en_US
dc.identifier.citationImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII; San Francisco, CA., 3-6 February 2014. In Proceedings of SPIE, 2014, v. 8947, article no. 894726en_US
dc.identifier.isbn978-081949860-1-
dc.identifier.issn1605-7422-
dc.identifier.urihttp://hdl.handle.net/10722/201229-
dc.description.abstractContinuing desire for higher-speed laser scanning fluorescence microscopy (LSFM) and progressive advancement in ultrafast and sensitive photodetectors might imply that our conventional understanding of LSFM is not adequate when approaching to the intrinsic speed limit - fluorescence lifetime. In this regard, we here revisit the theoretical framework of LSFM and evaluate its general performance in lifetime-limited and noise-limited regimes. Our model suggests that there still exists an order-of-magnitude gap between the current LSFM speed and the intrinsic limit. An imaging frame rate of > 100 kHz could be viable with the emerging laser-scanning techniques using ultrafast wavelength-swept sources, or optical time-stretch. © 2014 SPIE.-
dc.languageengen_US
dc.publisherSPIE - International Society for Optical Engineering.-
dc.relation.ispartofProgress in Biomedical Optics and Imaging: Proceedings of the SPIEen_US
dc.rightsProgress in Biomedical Optics and Imaging: Proceedings of the SPIE. Copyright © SPIE - International Society for Optical Engineering.-
dc.subjectImage resolution-
dc.subjectLaser scanning fluorescence microscopy-
dc.subjectSignal-to-noise ratio-
dc.subjectSpeed limit-
dc.titleRevisit laser scanning fluorescence microscopy performance under fluorescence-lifetime-limited regimeen_US
dc.typeConference_Paperen_US
dc.identifier.emailWong, KKY: kywong04@hkucc.hku.hken_US
dc.identifier.emailLam, EYM: elam@eee.hku.hken_US
dc.identifier.emailTsia, KKM: tsia@hku.hken_US
dc.identifier.authorityWong, KKY=rp00189en_US
dc.identifier.authorityLam, EYM=rp00131en_US
dc.identifier.authorityTsia, KKM=rp01389en_US
dc.identifier.doi10.1117/12.2038766-
dc.identifier.scopuseid_2-s2.0-84901779012-
dc.identifier.hkuros233699en_US
dc.identifier.volume8947-
dc.publisher.placeUnited States-

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