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Article: Unraveling the molecular basis of temperature-dependent genetic regulation in Penicillium marneffei

TitleUnraveling the molecular basis of temperature-dependent genetic regulation in Penicillium marneffei
Authors
Issue Date2013
PublisherAmerican Society for Microbiology.
Citation
Eukaryotic Cell , 2013, v. 12 n. 9, p. 1214-1224 How to Cite?
AbstractPenicillium marneffei is an opportunistic fungal pathogen endemic in Southeast Asia, causing lethal systemic infections in immunocompromised patients. P. marneffei grows in a mycelial form at the ambient temperature of 25 degrees C and transitions to a yeast form at 37 degrees C. The ability to alternate between the mycelial and yeast forms at different temperatures, namely, thermal dimorphism, has long been considered critical for the pathogenicity of P. marneffei, yet the underlying genetic mechanisms remain elusive. Here we employed high-throughput sequencing to unravel global transcriptional profiles of P. marneffei PM1 grown at 25 and 37 degrees C. Among approximately 11,000 protein-coding genes, 1,447 were overexpressed and 1,414 were underexpressed at 37 degrees C. Counterintuitively, heat-responsive genes, predicted in P. marneffei through sequence comparison, did not tend to be overexpressed at 37 degrees C. These results suggest that P. marneffei may take a distinct strategy of genetic regulation at the elevated temperature; the current knowledge concerning fungal heat response, based on studies of model fungal organisms, may not be applicable to P. marneffei. Our results further showed that the tandem repeat sequences (TRSs) are overrepresented in coding regions of P. marneffei genes, and TRS-containing genes tend to be overexpressed at 37 degrees C. Furthermore, genomic sequences and expression data were integrated to characterize gene clusters, multigene families, and species-specific genes of P. marneffei. In sum, we present an integrated analysis and a comprehensive resource toward a better understanding of temperature-dependent genetic regulation in P. marneffei.
Persistent Identifierhttp://hdl.handle.net/10722/200746
ISSN
2015 Impact Factor: 2.946
2015 SCImago Journal Rankings: 1.918
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorYang, E-
dc.contributor.authorWang, G-
dc.contributor.authorWoo, PCY-
dc.contributor.authorLau, SKP-
dc.contributor.authorChow, WN-
dc.contributor.authorChong, KTK-
dc.contributor.authorTse, H-
dc.contributor.authorKao, RYT-
dc.contributor.authorChan, CM-
dc.contributor.authorChe, X-
dc.contributor.authorYuen, KY-
dc.contributor.authorCai, JJ-
dc.date.accessioned2014-08-21T06:58:11Z-
dc.date.available2014-08-21T06:58:11Z-
dc.date.issued2013-
dc.identifier.citationEukaryotic Cell , 2013, v. 12 n. 9, p. 1214-1224-
dc.identifier.issn1535-9778-
dc.identifier.urihttp://hdl.handle.net/10722/200746-
dc.description.abstractPenicillium marneffei is an opportunistic fungal pathogen endemic in Southeast Asia, causing lethal systemic infections in immunocompromised patients. P. marneffei grows in a mycelial form at the ambient temperature of 25 degrees C and transitions to a yeast form at 37 degrees C. The ability to alternate between the mycelial and yeast forms at different temperatures, namely, thermal dimorphism, has long been considered critical for the pathogenicity of P. marneffei, yet the underlying genetic mechanisms remain elusive. Here we employed high-throughput sequencing to unravel global transcriptional profiles of P. marneffei PM1 grown at 25 and 37 degrees C. Among approximately 11,000 protein-coding genes, 1,447 were overexpressed and 1,414 were underexpressed at 37 degrees C. Counterintuitively, heat-responsive genes, predicted in P. marneffei through sequence comparison, did not tend to be overexpressed at 37 degrees C. These results suggest that P. marneffei may take a distinct strategy of genetic regulation at the elevated temperature; the current knowledge concerning fungal heat response, based on studies of model fungal organisms, may not be applicable to P. marneffei. Our results further showed that the tandem repeat sequences (TRSs) are overrepresented in coding regions of P. marneffei genes, and TRS-containing genes tend to be overexpressed at 37 degrees C. Furthermore, genomic sequences and expression data were integrated to characterize gene clusters, multigene families, and species-specific genes of P. marneffei. In sum, we present an integrated analysis and a comprehensive resource toward a better understanding of temperature-dependent genetic regulation in P. marneffei.-
dc.languageeng-
dc.publisherAmerican Society for Microbiology.-
dc.relation.ispartofEukaryotic Cell-
dc.rightsEukaryotic Cell . Copyright © American Society for Microbiology.-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subject.meshGene Expression Regulation, Fungal-
dc.subject.meshHeat-Shock Response - genetics-
dc.subject.meshPenicillium - genetics - metabolism-
dc.subject.meshTranscription, Genetic-
dc.titleUnraveling the molecular basis of temperature-dependent genetic regulation in Penicillium marneffei-
dc.typeArticle-
dc.identifier.emailWoo, PCY: pcywoo@hkucc.hku.hk-
dc.identifier.emailLau, SKP: skplau@hkucc.hku.hk-
dc.identifier.emailChow, WN: chow5810@hku.hk-
dc.identifier.emailChong, KTK: chongtk@hkucc.hku.hk-
dc.identifier.emailTse, H: herman@graduate.hku.hk-
dc.identifier.emailKao, RYT: rytkao@hkucc.hku.hk-
dc.identifier.emailChan, CM: cmchan@hku.hk-
dc.identifier.emailYuen, KY: kyyuen@hkucc.hku.hk-
dc.identifier.authorityWoo, PCY=rp00430-
dc.identifier.authorityLau, SKP=rp00486-
dc.identifier.authorityChow, WN=rp02493-
dc.identifier.authorityTse, H=rp00519-
dc.identifier.authorityKao, RYT=rp00481-
dc.identifier.authorityYuen, KY=rp00366-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1128/EC.00159-13-
dc.identifier.pmid23851338-
dc.identifier.pmcidPMC3811563-
dc.identifier.scopuseid_2-s2.0-84883211743-
dc.identifier.hkuros234963-
dc.identifier.volume12-
dc.identifier.issue9-
dc.identifier.spage1214-
dc.identifier.epage1224-
dc.identifier.isiWOS:000323756100006-
dc.publisher.placeUnited States-

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