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Article: Interferometric time-stretch microscopy for ultrafast quantitative cellular and tissue imaging at 1 μm

TitleInterferometric time-stretch microscopy for ultrafast quantitative cellular and tissue imaging at 1 μm
Authors
Issue Date2014
PublisherSPIE - International Society for Optical Engineering. The Journal's web site is located at http://spie.org/jbo
Citation
Journal of Biomedical Optics, 2014, v. 19 n. 7, article no. 076001, p. 076001-1-076001-7 How to Cite?
AbstractQuantitative phase imaging (QPI) has been proven to be a powerful tool for label-free characterization of biological specimens. However, the imaging speed, largely limited by the image sensor technology, impedes its utility in applications where high-throughput screening and efficient big-data analysis are mandated. We here demonstrate interferometric time-stretch (iTS) microscopy for delivering ultrafast quantitative phase cellular and tissue imaging at an imaging line-scan rate >20 MHz-orders-of-magnitude faster than conventional QPI. Enabling an efficient time-stretch operation in the 1-mum wavelength window, we present an iTS microscope system for practical ultrafast QPI of fixed cells and tissue sections, as well as ultrafast flowing cells (at a flow speed of up to 8 ms). To the best of our knowledge, this is the first time that time-stretch imaging could reveal quantitative morphological information of cells and tissues with nanometer precision. As many parameters can be further extracted from the phase and can serve as the intrinsic biomarkers for disease diagnosis, iTS microscopy could find its niche in high-throughput and high-content cellular assays (e.g., imaging flow cytometry) as well as tissue refractometric imaging (e.g., whole-slide imaging for digital pathology).
Persistent Identifierhttp://hdl.handle.net/10722/200609
ISSN
2015 Impact Factor: 2.556
2015 SCImago Journal Rankings: 1.201
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLau, KSAen_US
dc.contributor.authorWONG, TTWen_US
dc.contributor.authorHo, KYKen_US
dc.contributor.authorTang, MTHen_US
dc.contributor.authorCHAN, ACSen_US
dc.contributor.authorWEI, Xen_US
dc.contributor.authorLam, EYMen_US
dc.contributor.authorShum, HCen_US
dc.contributor.authorWong, KKYen_US
dc.contributor.authorTsia, KKMen_US
dc.date.accessioned2014-08-21T06:52:39Z-
dc.date.available2014-08-21T06:52:39Z-
dc.date.issued2014en_US
dc.identifier.citationJournal of Biomedical Optics, 2014, v. 19 n. 7, article no. 076001, p. 076001-1-076001-7en_US
dc.identifier.issn1083-3668-
dc.identifier.urihttp://hdl.handle.net/10722/200609-
dc.description.abstractQuantitative phase imaging (QPI) has been proven to be a powerful tool for label-free characterization of biological specimens. However, the imaging speed, largely limited by the image sensor technology, impedes its utility in applications where high-throughput screening and efficient big-data analysis are mandated. We here demonstrate interferometric time-stretch (iTS) microscopy for delivering ultrafast quantitative phase cellular and tissue imaging at an imaging line-scan rate >20 MHz-orders-of-magnitude faster than conventional QPI. Enabling an efficient time-stretch operation in the 1-mum wavelength window, we present an iTS microscope system for practical ultrafast QPI of fixed cells and tissue sections, as well as ultrafast flowing cells (at a flow speed of up to 8 ms). To the best of our knowledge, this is the first time that time-stretch imaging could reveal quantitative morphological information of cells and tissues with nanometer precision. As many parameters can be further extracted from the phase and can serve as the intrinsic biomarkers for disease diagnosis, iTS microscopy could find its niche in high-throughput and high-content cellular assays (e.g., imaging flow cytometry) as well as tissue refractometric imaging (e.g., whole-slide imaging for digital pathology).-
dc.languageengen_US
dc.publisherSPIE - International Society for Optical Engineering. The Journal's web site is located at http://spie.org/jbo-
dc.relation.ispartofJournal of Biomedical Opticsen_US
dc.rightsJournal of Biomedical Optics. Copyright © SPIE - International Society for Optical Engineering.-
dc.rightsCopyright notice format: Copyright 2014 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titleInterferometric time-stretch microscopy for ultrafast quantitative cellular and tissue imaging at 1 μmen_US
dc.typeArticleen_US
dc.identifier.emailLau, KSA: andylks@hku.hken_US
dc.identifier.emailHo, KYK: kennetho@hku.hken_US
dc.identifier.emailLam, EYM: elam@eee.hku.hken_US
dc.identifier.emailShum, HC: ashum@hku.hken_US
dc.identifier.emailWong, KKY: kywong04@hkucc.hku.hken_US
dc.identifier.emailTsia, KKM: tsia@hku.hken_US
dc.identifier.authorityLam, EYM=rp00131en_US
dc.identifier.authorityShum, HC=rp01439en_US
dc.identifier.authorityWong, KKY=rp00189en_US
dc.identifier.authorityTsia, KKM=rp01389en_US
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1117/1.JBO.19.7.076001-
dc.identifier.pmid24983913-
dc.identifier.hkuros233047en_US
dc.identifier.volume19en_US
dc.identifier.issue7en_US
dc.identifier.spage076001-1en_US
dc.identifier.epage076001-7en_US
dc.identifier.isiWOS:000340490400025-
dc.publisher.placeUnited States-

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