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Article: A novel dirofilaria species causing human and canine infections in Hong Kong

TitleA novel dirofilaria species causing human and canine infections in Hong Kong
Authors
Issue Date2012
Citation
Journal of Clinical Microbiology, 2012, v. 50, n. 11, p. 3534-3541 How to Cite?
AbstractDirofilariasis is globally the commonest manifestation of zoonotic filariasis. We report the detection of a novel canine species causing human and canine dirofilariasis in Hong Kong. Three human cases occurring over 10 months were identified, one presenting with cervical lymphadenopathy, one with an abdominal subcutaneous mass, and one with a subconjunctival nodule. Transected worms recovered from the resected abdominal subcutaneous mass were morphologically compatible with Dirofilaria. The cox1 gene sequences of the three human isolates were identical; however, they were only 96.2% and 89.3% identical to the cox1 gene of Dirofilaria repens and Dirofilaria immitis, respectively. Sequencing of the 18S-ITS1-5.8S gene cluster was successful in the intact worm, and the nucleotide sequences were 94.0% and 94.9% identical to those of D. repens and D. immitis, respectively. Screening of the blood samples from 200 dogs and 100 cats showed the presence of the novel Dirofilaria species in 3% (6/200) of the dogs' but none of the cats' blood samples. Nucleotide sequences of the cox1 gene and 18S-ITS1-5.8S gene clusters of the dogs' samples were identical to those in the human samples. The sera of canines infected by this novel Dirofilaria species were negative when tested with the SNAP 4Dx D. immitis detection kit, except in the case of dogs with a mixed infection with D. immitis as detected by PCR. The results from this study suggest that this novel Dirofilaria species is a cause of filarial infection in humans and dogs in Hong Kong. We propose to name this Dirofilaria species "Candidatus Dirofilaria hongkongensis." Copyright © 2012, American Society for Microbiology. All Rights Reserved.
Persistent Identifierhttp://hdl.handle.net/10722/200109
ISSN
2015 Impact Factor: 3.631
2015 SCImago Journal Rankings: 2.151
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorTo, Kelvin Kai Wang-
dc.contributor.authorWong, Samson Sai Yin-
dc.contributor.authorPoon, Rosana Wing Shan-
dc.contributor.authorTrendell-Smith, Nigel Jeremy-
dc.contributor.authorNgan, Antonio H Y-
dc.contributor.authorLam, Jacky W K-
dc.contributor.authorTang, Tommy H C-
dc.contributor.authorAhChong, Ah Kian-
dc.contributor.authorKan, Joshua Chi Hang-
dc.contributor.authorChan, Kwokhung-
dc.contributor.authorYuen, Kwok Yung-
dc.date.accessioned2014-07-26T23:11:09Z-
dc.date.available2014-07-26T23:11:09Z-
dc.date.issued2012-
dc.identifier.citationJournal of Clinical Microbiology, 2012, v. 50, n. 11, p. 3534-3541-
dc.identifier.issn0095-1137-
dc.identifier.urihttp://hdl.handle.net/10722/200109-
dc.description.abstractDirofilariasis is globally the commonest manifestation of zoonotic filariasis. We report the detection of a novel canine species causing human and canine dirofilariasis in Hong Kong. Three human cases occurring over 10 months were identified, one presenting with cervical lymphadenopathy, one with an abdominal subcutaneous mass, and one with a subconjunctival nodule. Transected worms recovered from the resected abdominal subcutaneous mass were morphologically compatible with Dirofilaria. The cox1 gene sequences of the three human isolates were identical; however, they were only 96.2% and 89.3% identical to the cox1 gene of Dirofilaria repens and Dirofilaria immitis, respectively. Sequencing of the 18S-ITS1-5.8S gene cluster was successful in the intact worm, and the nucleotide sequences were 94.0% and 94.9% identical to those of D. repens and D. immitis, respectively. Screening of the blood samples from 200 dogs and 100 cats showed the presence of the novel Dirofilaria species in 3% (6/200) of the dogs' but none of the cats' blood samples. Nucleotide sequences of the cox1 gene and 18S-ITS1-5.8S gene clusters of the dogs' samples were identical to those in the human samples. The sera of canines infected by this novel Dirofilaria species were negative when tested with the SNAP 4Dx D. immitis detection kit, except in the case of dogs with a mixed infection with D. immitis as detected by PCR. The results from this study suggest that this novel Dirofilaria species is a cause of filarial infection in humans and dogs in Hong Kong. We propose to name this Dirofilaria species "Candidatus Dirofilaria hongkongensis." Copyright © 2012, American Society for Microbiology. All Rights Reserved.-
dc.languageeng-
dc.relation.ispartofJournal of Clinical Microbiology-
dc.titleA novel dirofilaria species causing human and canine infections in Hong Kong-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1128/JCM.01590-12-
dc.identifier.pmid22915604-
dc.identifier.scopuseid_2-s2.0-84867525358-
dc.identifier.volume50-
dc.identifier.issue11-
dc.identifier.spage3534-
dc.identifier.epage3541-
dc.identifier.eissn1098-660X-
dc.identifier.isiWOS:000309966900020-

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