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Article: Heterogeneity of the prtC gene of Porphyromonas gingivalis

TitleHeterogeneity of the prtC gene of Porphyromonas gingivalis
Authors
KeywordsEpidemiology
PrtC
Porphyromonas gingivalis
Restriction fragment-length polymorphism
Heterogeneity
Issue Date2000
Citation
Oral Microbiology and Immunology, 2000, v. 15, n. 1, p. 33-39 How to Cite?
AbstractIn this study, the nucleotide sequences of the prtC genes of six clinical Porphyromonas gingivalis isolates obtained from patients with periodontitis and from reference strain 53977 were determined. All analyzed genes were heterogeneous in their nucleotide composition and differed in up to 13 nucleotides. Moreover, substantial differences were found in comparison to prtC of reference strain 53977. The prtC genes of 45 Porphyromonas gingivalis isolates were amplified by polymerase chain reaction (PCR) and the PCR products were also digested with restriction endonucleases Tsp509I, NlaIII and DraII (PCR-restriction fragment-length polymorphism). Nine different restriction pattern combinations were observed, with four being most frequent (28.9%, 26.7%, 17.8% and 11.1%). The data presented here demonstrates that prtC genes are heterogeneous in their nucleotide sequence and therefore may be used as a target for molecular epidemiological studies. The observed heterogeneity of prtC genes may be a result of microevolution processes.
Persistent Identifierhttp://hdl.handle.net/10722/200077
ISSN
2011 Impact Factor: 2.807
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWittstock, Marcus-
dc.contributor.authorSchmidt, Herbert H.-
dc.contributor.authorFlemmig, Thomas Frank-
dc.contributor.authorKarch, Helge W.-
dc.date.accessioned2014-07-26T23:11:06Z-
dc.date.available2014-07-26T23:11:06Z-
dc.date.issued2000-
dc.identifier.citationOral Microbiology and Immunology, 2000, v. 15, n. 1, p. 33-39-
dc.identifier.issn0902-0055-
dc.identifier.urihttp://hdl.handle.net/10722/200077-
dc.description.abstractIn this study, the nucleotide sequences of the prtC genes of six clinical Porphyromonas gingivalis isolates obtained from patients with periodontitis and from reference strain 53977 were determined. All analyzed genes were heterogeneous in their nucleotide composition and differed in up to 13 nucleotides. Moreover, substantial differences were found in comparison to prtC of reference strain 53977. The prtC genes of 45 Porphyromonas gingivalis isolates were amplified by polymerase chain reaction (PCR) and the PCR products were also digested with restriction endonucleases Tsp509I, NlaIII and DraII (PCR-restriction fragment-length polymorphism). Nine different restriction pattern combinations were observed, with four being most frequent (28.9%, 26.7%, 17.8% and 11.1%). The data presented here demonstrates that prtC genes are heterogeneous in their nucleotide sequence and therefore may be used as a target for molecular epidemiological studies. The observed heterogeneity of prtC genes may be a result of microevolution processes.-
dc.languageeng-
dc.relation.ispartofOral Microbiology and Immunology-
dc.subjectEpidemiology-
dc.subjectPrtC-
dc.subjectPorphyromonas gingivalis-
dc.subjectRestriction fragment-length polymorphism-
dc.subjectHeterogeneity-
dc.titleHeterogeneity of the prtC gene of Porphyromonas gingivalis-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1034/j.1399-302X.2000.150106.x-
dc.identifier.pmid11155162-
dc.identifier.scopuseid_2-s2.0-0033959873-
dc.identifier.volume15-
dc.identifier.issue1-
dc.identifier.spage33-
dc.identifier.epage39-
dc.identifier.isiWOS:000084918900006-

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