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- Publisher Website: 10.1128/JCM.34.10.2411-2413.1996
- Scopus: eid_2-s2.0-0029820917
- PMID: 8880490
- WOS: WOS:A1996VK78700014
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Article: Serodiagnosis of Porphyromonas gingivalis infection by immunoblot analysis with recombinant collagenase
Title | Serodiagnosis of Porphyromonas gingivalis infection by immunoblot analysis with recombinant collagenase |
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Authors | |
Issue Date | 1996 |
Citation | Journal of Clinical Microbiology, 1996, v. 34, n. 10, p. 2411-2413 How to Cite? |
Abstract | The Porphyromonas gingivalis collagenase-specific serum immunoglobulin A (IgA), IgM, and IgG responses from 20 patients with early-onset periodontitis (EOP), 20 patients with adult periodontitis (AP), and 20 age- and sex- matched healthy controls were examined by immunoblot analysis. A recombinant collagenase antigen used for the immunoblot analysis was produced by using the plasmid pGEX-2T, which allows the fusion between the collagenase and glutathione S-transferase. There was no significant difference in collagenase-specific IgG antibody detection between samples from the EOP, AP, and control groups. In contrast, 85% of AP and EOP sera had collagenase- specific IgA antibodies, whereas only 20% of control sera showed collagenase- specific IgA reactivity. Plaque samples from all groups were assessed by PCR with primers complementary to the collagenase-encoding gene prtC. The results indicated that 90% of AP and EOP plaque samples and 10% of control samples were positive for P. gingivalis. All patients with collagenase-specific IgA antibodies were PCR positive. The results of the study indicate a nearly complete concordance (k = 0.856) between the presence of collagenase-specific IgA antibodies and PCR detection of P. gingivalis. By using PCR as the 'gold standard,' the sensitivity and specificity of the IgA immunoblot test were 94.7 and 90.9%, respectively. Therefore, the recombinant collagenase is a potential candidate for use in the serodiagnosis of periodontitis. |
Persistent Identifier | http://hdl.handle.net/10722/200051 |
ISSN | 2023 Impact Factor: 6.1 2023 SCImago Journal Rankings: 1.653 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Wittstock, Marcus | - |
dc.contributor.author | Flemmig, Thomas Frank | - |
dc.contributor.author | Schmidt, Herbert H. | - |
dc.contributor.author | Mutters, Reinier | - |
dc.contributor.author | Karch, Helge W. | - |
dc.date.accessioned | 2014-07-26T23:11:04Z | - |
dc.date.available | 2014-07-26T23:11:04Z | - |
dc.date.issued | 1996 | - |
dc.identifier.citation | Journal of Clinical Microbiology, 1996, v. 34, n. 10, p. 2411-2413 | - |
dc.identifier.issn | 0095-1137 | - |
dc.identifier.uri | http://hdl.handle.net/10722/200051 | - |
dc.description.abstract | The Porphyromonas gingivalis collagenase-specific serum immunoglobulin A (IgA), IgM, and IgG responses from 20 patients with early-onset periodontitis (EOP), 20 patients with adult periodontitis (AP), and 20 age- and sex- matched healthy controls were examined by immunoblot analysis. A recombinant collagenase antigen used for the immunoblot analysis was produced by using the plasmid pGEX-2T, which allows the fusion between the collagenase and glutathione S-transferase. There was no significant difference in collagenase-specific IgG antibody detection between samples from the EOP, AP, and control groups. In contrast, 85% of AP and EOP sera had collagenase- specific IgA antibodies, whereas only 20% of control sera showed collagenase- specific IgA reactivity. Plaque samples from all groups were assessed by PCR with primers complementary to the collagenase-encoding gene prtC. The results indicated that 90% of AP and EOP plaque samples and 10% of control samples were positive for P. gingivalis. All patients with collagenase-specific IgA antibodies were PCR positive. The results of the study indicate a nearly complete concordance (k = 0.856) between the presence of collagenase-specific IgA antibodies and PCR detection of P. gingivalis. By using PCR as the 'gold standard,' the sensitivity and specificity of the IgA immunoblot test were 94.7 and 90.9%, respectively. Therefore, the recombinant collagenase is a potential candidate for use in the serodiagnosis of periodontitis. | - |
dc.language | eng | - |
dc.relation.ispartof | Journal of Clinical Microbiology | - |
dc.title | Serodiagnosis of Porphyromonas gingivalis infection by immunoblot analysis with recombinant collagenase | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1128/JCM.34.10.2411-2413.1996 | - |
dc.identifier.pmid | 8880490 | - |
dc.identifier.scopus | eid_2-s2.0-0029820917 | - |
dc.identifier.volume | 34 | - |
dc.identifier.issue | 10 | - |
dc.identifier.spage | 2411 | - |
dc.identifier.epage | 2413 | - |
dc.identifier.isi | WOS:A1996VK78700014 | - |
dc.identifier.issnl | 0095-1137 | - |