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Article: Serodiagnosis of Porphyromonas gingivalis infection by immunoblot analysis with recombinant collagenase

TitleSerodiagnosis of Porphyromonas gingivalis infection by immunoblot analysis with recombinant collagenase
Authors
Issue Date1996
Citation
Journal of Clinical Microbiology, 1996, v. 34, n. 10, p. 2411-2413 How to Cite?
AbstractThe Porphyromonas gingivalis collagenase-specific serum immunoglobulin A (IgA), IgM, and IgG responses from 20 patients with early-onset periodontitis (EOP), 20 patients with adult periodontitis (AP), and 20 age- and sex- matched healthy controls were examined by immunoblot analysis. A recombinant collagenase antigen used for the immunoblot analysis was produced by using the plasmid pGEX-2T, which allows the fusion between the collagenase and glutathione S-transferase. There was no significant difference in collagenase-specific IgG antibody detection between samples from the EOP, AP, and control groups. In contrast, 85% of AP and EOP sera had collagenase- specific IgA antibodies, whereas only 20% of control sera showed collagenase- specific IgA reactivity. Plaque samples from all groups were assessed by PCR with primers complementary to the collagenase-encoding gene prtC. The results indicated that 90% of AP and EOP plaque samples and 10% of control samples were positive for P. gingivalis. All patients with collagenase-specific IgA antibodies were PCR positive. The results of the study indicate a nearly complete concordance (k = 0.856) between the presence of collagenase-specific IgA antibodies and PCR detection of P. gingivalis. By using PCR as the 'gold standard,' the sensitivity and specificity of the IgA immunoblot test were 94.7 and 90.9%, respectively. Therefore, the recombinant collagenase is a potential candidate for use in the serodiagnosis of periodontitis.
Persistent Identifierhttp://hdl.handle.net/10722/200051
ISSN
2015 Impact Factor: 3.631
2015 SCImago Journal Rankings: 2.151
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWittstock, Marcus-
dc.contributor.authorFlemmig, Thomas Frank-
dc.contributor.authorSchmidt, Herbert H.-
dc.contributor.authorMutters, Reinier-
dc.contributor.authorKarch, Helge W.-
dc.date.accessioned2014-07-26T23:11:04Z-
dc.date.available2014-07-26T23:11:04Z-
dc.date.issued1996-
dc.identifier.citationJournal of Clinical Microbiology, 1996, v. 34, n. 10, p. 2411-2413-
dc.identifier.issn0095-1137-
dc.identifier.urihttp://hdl.handle.net/10722/200051-
dc.description.abstractThe Porphyromonas gingivalis collagenase-specific serum immunoglobulin A (IgA), IgM, and IgG responses from 20 patients with early-onset periodontitis (EOP), 20 patients with adult periodontitis (AP), and 20 age- and sex- matched healthy controls were examined by immunoblot analysis. A recombinant collagenase antigen used for the immunoblot analysis was produced by using the plasmid pGEX-2T, which allows the fusion between the collagenase and glutathione S-transferase. There was no significant difference in collagenase-specific IgG antibody detection between samples from the EOP, AP, and control groups. In contrast, 85% of AP and EOP sera had collagenase- specific IgA antibodies, whereas only 20% of control sera showed collagenase- specific IgA reactivity. Plaque samples from all groups were assessed by PCR with primers complementary to the collagenase-encoding gene prtC. The results indicated that 90% of AP and EOP plaque samples and 10% of control samples were positive for P. gingivalis. All patients with collagenase-specific IgA antibodies were PCR positive. The results of the study indicate a nearly complete concordance (k = 0.856) between the presence of collagenase-specific IgA antibodies and PCR detection of P. gingivalis. By using PCR as the 'gold standard,' the sensitivity and specificity of the IgA immunoblot test were 94.7 and 90.9%, respectively. Therefore, the recombinant collagenase is a potential candidate for use in the serodiagnosis of periodontitis.-
dc.languageeng-
dc.relation.ispartofJournal of Clinical Microbiology-
dc.titleSerodiagnosis of Porphyromonas gingivalis infection by immunoblot analysis with recombinant collagenase-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid8880490-
dc.identifier.scopuseid_2-s2.0-0029820917-
dc.identifier.volume34-
dc.identifier.issue10-
dc.identifier.spage2411-
dc.identifier.epage2413-
dc.identifier.isiWOS:A1996VK78700014-

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