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Article: Characterization and specificity controls of murine monoclonal antibodies against serogroup C1 Salmonella

TitleCharacterization and specificity controls of murine monoclonal antibodies against serogroup C1 Salmonella
Authors
Issue Date1992
Citation
Diagnostic Microbiology and Infectious Disease, 1992, v. 15, n. 3, p. 213-223 How to Cite?
AbstractTwo IgG3 murine monoclonal antibodies, Cl-1 and Cl-2, that showed serologic specificities for the O antigens of serogroup C1 (O:6,6) Salmonella were established. The epitopes for the antibodies were found to reside on the repeating units of the serogroup C1 Salmonella lipopolysaccharide and were labile to sodium metaperiodate oxidation. Serologic reactivities of Cl-1 and Cl-2 were not inhibited by commercial monospecific antiserum to O antigen 7, but were inhibited to various degrees by anti-[O:6,7] serum. Both antibodies reacted strongly with all strains of serogroup C1 Salmonella that have either O:61,7, O:62,7, or O:61,2,7 antigens. Reactivities of Cl-1 and Cl-2 with the phage-14 lysogenized C1 strains that bear the phage-modified O antigen (O:6,7 → O:6,7,14) were detected by slide agglutination method only and not by wholecell enzyme-linked immunosorbent assay. Both Cl-1 and Cl-2 antibodies did not react with other O serogroups of salmonellae, nor with other Gram-negative or Gram-positive bacteria. The diagnostic value of these monoclonal antibodies together with a previously described monoclonal antibody against the serogroup C2 Salmonella was demonstrated using the slide agglutination method with monoclonal antibodies ascitic fluids. © 1992.
Persistent Identifierhttp://hdl.handle.net/10722/200042
ISSN
2015 Impact Factor: 2.45
2015 SCImago Journal Rankings: 1.142
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorTsang, Raymond-
dc.contributor.authorAleksić, Stojanka-
dc.contributor.authorChan, Kwokhung-
dc.contributor.authorLau, N. W H-
dc.contributor.authorNg, Muni H.-
dc.date.accessioned2014-07-26T23:11:04Z-
dc.date.available2014-07-26T23:11:04Z-
dc.date.issued1992-
dc.identifier.citationDiagnostic Microbiology and Infectious Disease, 1992, v. 15, n. 3, p. 213-223-
dc.identifier.issn0732-8893-
dc.identifier.urihttp://hdl.handle.net/10722/200042-
dc.description.abstractTwo IgG3 murine monoclonal antibodies, Cl-1 and Cl-2, that showed serologic specificities for the O antigens of serogroup C1 (O:6,6) Salmonella were established. The epitopes for the antibodies were found to reside on the repeating units of the serogroup C1 Salmonella lipopolysaccharide and were labile to sodium metaperiodate oxidation. Serologic reactivities of Cl-1 and Cl-2 were not inhibited by commercial monospecific antiserum to O antigen 7, but were inhibited to various degrees by anti-[O:6,7] serum. Both antibodies reacted strongly with all strains of serogroup C1 Salmonella that have either O:61,7, O:62,7, or O:61,2,7 antigens. Reactivities of Cl-1 and Cl-2 with the phage-14 lysogenized C1 strains that bear the phage-modified O antigen (O:6,7 → O:6,7,14) were detected by slide agglutination method only and not by wholecell enzyme-linked immunosorbent assay. Both Cl-1 and Cl-2 antibodies did not react with other O serogroups of salmonellae, nor with other Gram-negative or Gram-positive bacteria. The diagnostic value of these monoclonal antibodies together with a previously described monoclonal antibody against the serogroup C2 Salmonella was demonstrated using the slide agglutination method with monoclonal antibodies ascitic fluids. © 1992.-
dc.languageeng-
dc.relation.ispartofDiagnostic Microbiology and Infectious Disease-
dc.titleCharacterization and specificity controls of murine monoclonal antibodies against serogroup C1 Salmonella-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/0732-8893(92)90116-B-
dc.identifier.pmid1374696-
dc.identifier.scopuseid_2-s2.0-0026596744-
dc.identifier.volume15-
dc.identifier.issue3-
dc.identifier.spage213-
dc.identifier.epage223-
dc.identifier.isiWOS:A1992HM34200005-

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