File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1084/jem.187.5.787
- Scopus: eid_2-s2.0-2642610290
- PMID: 9480988
- WOS: WOS:000072444700013
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Selective expression of a stable cell surface molecule on type 2 but not type 1 helper T cells
Title | Selective expression of a stable cell surface molecule on type 2 but not type 1 helper T cells |
---|---|
Authors | |
Issue Date | 1998 |
Citation | Journal of Experimental Medicine, 1998, v. 187, n. 5, p. 787-794 How to Cite? |
Abstract | T helper cell type 1 (Th1) and 2 (Th2) are central to immune regulation. However, no stable cell surface marker capable of distinguishing and separating these two subsets of CD4 + cells has yet been found. Using differential display PCR, we have identified a gene encoding a cell membrane bound molecule, originally designated ST2L, T1, DER4, or Fit, expressed constitutively and stably on the surface of murine Th2s, but not Th1s even after stimulation with a range of immunological stimuli. Antibody against a peptide derived from ST2L strongly and stably labeled the surface of cloned Th2s but not Th1s, and Th2s but not Th1s derived from naive T cells of ovalbumin T cell receptor-α/β transgenic mice. Three-color single cell flow cytometric analysis shows that cell surface ST2L coexpressed with intracellular interleukin (IL)-4, but not with interferon (IFN)-γ. The antibody selectively lysed Th2s in vitro in a complement-dependent manner. In vivo, it enhanced Th1 responses by increasing IFN-γ production and decreasing IL-4 and IL-5 synthesis. It induced resistance to Leishmania major infection in BALB/c mice and exacerbated collagen-induced arthritis in DBA/1 mice. Thus, ST2L is a stable marker distinguishing Th2s from Th1s and is also associated with Th2 functions. Hence, it may be a target for therapeutic intervention. |
Persistent Identifier | http://hdl.handle.net/10722/199907 |
ISSN | 2023 Impact Factor: 12.6 2023 SCImago Journal Rankings: 6.838 |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Xu, Damo | - |
dc.contributor.author | Chan, WoonLing | - |
dc.contributor.author | Leung, Bernard | - |
dc.contributor.author | Huang, Fangping | - |
dc.contributor.author | Wheeler, Rachel D. | - |
dc.contributor.author | Piedrafita, David M. | - |
dc.contributor.author | Robinson, John H. | - |
dc.contributor.author | Liew, Fooyew | - |
dc.date.accessioned | 2014-07-26T23:10:53Z | - |
dc.date.available | 2014-07-26T23:10:53Z | - |
dc.date.issued | 1998 | - |
dc.identifier.citation | Journal of Experimental Medicine, 1998, v. 187, n. 5, p. 787-794 | - |
dc.identifier.issn | 0022-1007 | - |
dc.identifier.uri | http://hdl.handle.net/10722/199907 | - |
dc.description.abstract | T helper cell type 1 (Th1) and 2 (Th2) are central to immune regulation. However, no stable cell surface marker capable of distinguishing and separating these two subsets of CD4 + cells has yet been found. Using differential display PCR, we have identified a gene encoding a cell membrane bound molecule, originally designated ST2L, T1, DER4, or Fit, expressed constitutively and stably on the surface of murine Th2s, but not Th1s even after stimulation with a range of immunological stimuli. Antibody against a peptide derived from ST2L strongly and stably labeled the surface of cloned Th2s but not Th1s, and Th2s but not Th1s derived from naive T cells of ovalbumin T cell receptor-α/β transgenic mice. Three-color single cell flow cytometric analysis shows that cell surface ST2L coexpressed with intracellular interleukin (IL)-4, but not with interferon (IFN)-γ. The antibody selectively lysed Th2s in vitro in a complement-dependent manner. In vivo, it enhanced Th1 responses by increasing IFN-γ production and decreasing IL-4 and IL-5 synthesis. It induced resistance to Leishmania major infection in BALB/c mice and exacerbated collagen-induced arthritis in DBA/1 mice. Thus, ST2L is a stable marker distinguishing Th2s from Th1s and is also associated with Th2 functions. Hence, it may be a target for therapeutic intervention. | - |
dc.language | eng | - |
dc.relation.ispartof | Journal of Experimental Medicine | - |
dc.title | Selective expression of a stable cell surface molecule on type 2 but not type 1 helper T cells | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1084/jem.187.5.787 | - |
dc.identifier.pmid | 9480988 | - |
dc.identifier.scopus | eid_2-s2.0-2642610290 | - |
dc.identifier.volume | 187 | - |
dc.identifier.issue | 5 | - |
dc.identifier.spage | 787 | - |
dc.identifier.epage | 794 | - |
dc.identifier.isi | WOS:000072444700013 | - |
dc.identifier.issnl | 0022-1007 | - |