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Article: Production of nitric oxide in the synovial membrane of rheumatoid and osteoarthritis patients

TitleProduction of nitric oxide in the synovial membrane of rheumatoid and osteoarthritis patients
Authors
Issue Date1996
Citation
Journal of Experimental Medicine, 1996, v. 184, n. 4, p. 1519-1524 How to Cite?
AbstractWe have demonstrated spontaneous nitric oxide (NO) production by primary synovial cultures from rheumatoid (RA) and osteoarthritis patients. Increased NO production followed addition of staphylococcal enterotoxin B. Immunochemical double staining with specific anti-human inducible NO synthase (iNOS) and nonspecific esterase (NSE), or anti-CD68 (markers for tissue macrophages) showed that although many lining layer cells in RA synovium expressed iNOS, most (~90%) were NSE+ and CD68+, with only a minor population (~10%) which were iNOS+, CD68+/NSE+. These data demonstrate the capacity for high output of NO by human synovial tissue and show that, although human macrophages can express high levels of iNOS, the majority of cells expressing iNOS are fibroblasts. We also report that synoviocytes, and macrophage cell lines, cultured with the NO donor, S-nitroso-acetyl penicillamine, produced high concentrations of tumor necrosis factor (TNF)- α. These results suggest that NO may mediate pathology in RA through the induction of TNF-α production.
Persistent Identifierhttp://hdl.handle.net/10722/199899
ISSN
2015 Impact Factor: 11.24
2015 SCImago Journal Rankings: 10.762

 

DC FieldValueLanguage
dc.contributor.authorMcInnes, Iain B.-
dc.contributor.authorLeung, Bernard-
dc.contributor.authorField, Max H.-
dc.contributor.authorWei, Xiaoqing-
dc.contributor.authorHuang, Fangping-
dc.contributor.authorSturrock, Roger D.-
dc.contributor.authorKinninmonth, Andrew WG G-
dc.contributor.authorWeidner, Jeffrey R.-
dc.contributor.authorMumford, Richard A.-
dc.contributor.authorLiew, Fooyew-
dc.date.accessioned2014-07-26T23:10:53Z-
dc.date.available2014-07-26T23:10:53Z-
dc.date.issued1996-
dc.identifier.citationJournal of Experimental Medicine, 1996, v. 184, n. 4, p. 1519-1524-
dc.identifier.issn0022-1007-
dc.identifier.urihttp://hdl.handle.net/10722/199899-
dc.description.abstractWe have demonstrated spontaneous nitric oxide (NO) production by primary synovial cultures from rheumatoid (RA) and osteoarthritis patients. Increased NO production followed addition of staphylococcal enterotoxin B. Immunochemical double staining with specific anti-human inducible NO synthase (iNOS) and nonspecific esterase (NSE), or anti-CD68 (markers for tissue macrophages) showed that although many lining layer cells in RA synovium expressed iNOS, most (~90%) were NSE+ and CD68+, with only a minor population (~10%) which were iNOS+, CD68+/NSE+. These data demonstrate the capacity for high output of NO by human synovial tissue and show that, although human macrophages can express high levels of iNOS, the majority of cells expressing iNOS are fibroblasts. We also report that synoviocytes, and macrophage cell lines, cultured with the NO donor, S-nitroso-acetyl penicillamine, produced high concentrations of tumor necrosis factor (TNF)- α. These results suggest that NO may mediate pathology in RA through the induction of TNF-α production.-
dc.languageeng-
dc.relation.ispartofJournal of Experimental Medicine-
dc.titleProduction of nitric oxide in the synovial membrane of rheumatoid and osteoarthritis patients-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1084/jem.184.4.1519-
dc.identifier.pmid8879223-
dc.identifier.scopuseid_2-s2.0-10244253892-
dc.identifier.volume184-
dc.identifier.issue4-
dc.identifier.spage1519-
dc.identifier.epage1524-

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