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Conference Paper: In-vitro agarose hydrogel system for biomimetic mineralisation on demineralised dentine

TitleIn-vitro agarose hydrogel system for biomimetic mineralisation on demineralised dentine
Authors
KeywordsCollagen
Dentin
Enamel
Regeneration and Remineralization
Issue Date2014
PublisherSage Publications, Inc. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925
Citation
The 92nd General Session & Exhibition of the International Association for Dental Research (IADR), Cape Town, South Africa, 25-28 June 2014. In Journal of Dental Research, 2014, v. 93 n. Special issue B: abstract no. 1207 How to Cite?
AbstractObjective: To develop an in vitro agarose hydrogel system for biomimetic mineralisation on demineralised dentine. Method: Human dentine slices were prepared from extracted sound human molars. They were polished and acid-etched with 37% phosphoric acid for 15 sec to create demineralized dentine surfaces. The slices were put into the polyethylene tubes with the demineralized dentine surface covered by a 2-mm-thick layer of calcium chloride (CaCl2) agarose hydrogel. Another 2-mm-thick layer of ion-free agarose hydrogel was added on top of the CaCl2 agarose hydrogel. They were immersed into a freshly prepared solution containing phosphate and fluoride and incubated at 37˚C for 6 days. The solution was replaced every 24 hours and the agarose hydrogels were replaced every 48 hours. Scanning electron microscopy (SEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) were used to evaluate the crystals formed on dentine surface. Nanoscratch test was carried out using a nano-indentor to evaluate the tribological property of the regenerated crystals with dentine. Result: Observation under SEM showed that the crystals occluded the dentinal tubules and an enamel prism-like tissue formed on the demineralized dentine surface. XRD and FTIR analyses suggested that the crystals were hydroxyapatite. No crack was observed in the nanoscratch test. Conclusion: In this study, an in vitro agarose hydrogel system was successfully developed to regenerate enamel prism-like tissue on demineralised dentine surface.
DescriptionPoster Presentation
Session 164: Emerging Knowledge on Pathogenesis and Management of Dental Caries
Persistent Identifierhttp://hdl.handle.net/10722/199335
ISSN
2015 Impact Factor: 4.602
2015 SCImago Journal Rankings: 1.714

 

DC FieldValueLanguage
dc.contributor.authorCao, Yen_US
dc.contributor.authorLi, QLen_US
dc.contributor.authorLo, ECMen_US
dc.contributor.authorChu, CHen_US
dc.date.accessioned2014-07-22T01:13:40Z-
dc.date.available2014-07-22T01:13:40Z-
dc.date.issued2014en_US
dc.identifier.citationThe 92nd General Session & Exhibition of the International Association for Dental Research (IADR), Cape Town, South Africa, 25-28 June 2014. In Journal of Dental Research, 2014, v. 93 n. Special issue B: abstract no. 1207en_US
dc.identifier.issn0022-0345en_US
dc.identifier.urihttp://hdl.handle.net/10722/199335-
dc.descriptionPoster Presentation-
dc.descriptionSession 164: Emerging Knowledge on Pathogenesis and Management of Dental Caries-
dc.description.abstractObjective: To develop an in vitro agarose hydrogel system for biomimetic mineralisation on demineralised dentine. Method: Human dentine slices were prepared from extracted sound human molars. They were polished and acid-etched with 37% phosphoric acid for 15 sec to create demineralized dentine surfaces. The slices were put into the polyethylene tubes with the demineralized dentine surface covered by a 2-mm-thick layer of calcium chloride (CaCl2) agarose hydrogel. Another 2-mm-thick layer of ion-free agarose hydrogel was added on top of the CaCl2 agarose hydrogel. They were immersed into a freshly prepared solution containing phosphate and fluoride and incubated at 37˚C for 6 days. The solution was replaced every 24 hours and the agarose hydrogels were replaced every 48 hours. Scanning electron microscopy (SEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) were used to evaluate the crystals formed on dentine surface. Nanoscratch test was carried out using a nano-indentor to evaluate the tribological property of the regenerated crystals with dentine. Result: Observation under SEM showed that the crystals occluded the dentinal tubules and an enamel prism-like tissue formed on the demineralized dentine surface. XRD and FTIR analyses suggested that the crystals were hydroxyapatite. No crack was observed in the nanoscratch test. Conclusion: In this study, an in vitro agarose hydrogel system was successfully developed to regenerate enamel prism-like tissue on demineralised dentine surface.-
dc.languageengen_US
dc.publisherSage Publications, Inc. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925en_US
dc.relation.ispartofJournal of Dental Researchen_US
dc.rightsJournal of Dental Research. Copyright © Sage Publications, Inc.en_US
dc.subjectCollagen-
dc.subjectDentin-
dc.subjectEnamel-
dc.subjectRegeneration and Remineralization-
dc.titleIn-vitro agarose hydrogel system for biomimetic mineralisation on demineralised dentineen_US
dc.typeConference_Paperen_US
dc.identifier.emailLo, ECM: edward-lo@hku.hken_US
dc.identifier.emailChu, CH: chchu@hku.hken_US
dc.identifier.authorityLo, ECM=rp00015en_US
dc.identifier.authorityChu, CH=rp00022en_US
dc.identifier.hkuros231074en_US
dc.identifier.volume93en_US
dc.identifier.issueSpecial issue B: abstract no. 1207en_US
dc.publisher.placeUnited Statesen_US

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