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Conference Paper: Etch-and-rinse versus Self-etch Pretreatment: the Ultra-structural Investigation on Intact Enamel

TitleEtch-and-rinse versus Self-etch Pretreatment: the Ultra-structural Investigation on Intact Enamel
Authors
KeywordsAcid etch
Dental materials
Enamel
Histology - ultrastructure and Self-etching primer
Issue Date2014
PublisherSage Publications, Inc.. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925
Citation
The 92nd General Session & Exhibition of the International Association for Dental Research (IADR), Cape Town, South Africa, 25-28 June 2014. In Journal of Dental Research, 2014, v. 93 n. Special issue B: abstract no. 1394 How to Cite?
AbstractObjective: To investigate the ultra-structural changes produced by phosphoric acid (PA) and self-etching primer (SEP) on intact enamel surface. Methods: Sound human teeth were collected with ethical approval (IRB No.: UW13-262) and randomly assigned to four groups: Group A (40% PA liquid), Group B (40% PA gel), Group C (Clearfil SE Bond primer, Kuraray Co.), and Group D (Transbond Plus SEP, 3M Unitek). Four coronal central buccal enamel specimens with surface dimension of 1x1.5 mm2 were sectioned and gently cleansed. In groups A and B, specimens were etched for 0, 15, 30, and 60 seconds, respectively, rinsed thoroughly with de-ionized water and dried afterwards. In groups C and D, SEPs were applied following manufacturer’s instructions. Specimens without treatment were set as control. Surface chemical composition, crystalline structure, and morphology were determined by energy dispersive x-ray spectroscopy (EDX), x-ray diffraction (XRD) and scanning electron microscopy (SEM). Results: Calcium to phosphate (Ca/P) ratio was calculated and analyzed by two-way analysis of variance (α=0.05). After PA-etch, Ca/P ratio was initially increased and then decreased, without significant differences among various etching durations (p>0.05). Statistical difference was demonstrated between liquid and gel (p=0.016). Application of SEP dramatically decreased the Ca/P ratio (p<0.001), which was also significantly influenced by the type of SEP (p=0.003). XRD spectra revealed existence of new phosphate compounds in all groups, while intensity changes of hydroxyapatite characteristic peaks were only found in groups A and B. SEM exhibited typical etch patterns generated by PA-etch. Whereas, very conservative etch pattern was produced by SEP, which masked enamel surface with a hybrid layer. Conclusion: PA-etch dissolved the superficial layer of intact enamel, changed the crystalline structure of enamel hydroxyapatite and efficiently roughed the surface. SEP had limited dissolution ability and less actively reacted with enamel substrate. However, new phosphate compounds were formed by either technique.
DescriptionOral Presentation
Session 193: Keynote Address; Adhesive Interaction with Enamel, Dentin and New Materials
Persistent Identifierhttp://hdl.handle.net/10722/199315
ISSN
2015 Impact Factor: 4.602
2015 SCImago Journal Rankings: 1.714

 

DC FieldValueLanguage
dc.contributor.authorZhu, Jen_US
dc.contributor.authorTsoi, KHen_US
dc.contributor.authorTang, ATHen_US
dc.contributor.authorHagg, EUOen_US
dc.contributor.authorMatinlinna, JPen_US
dc.date.accessioned2014-07-22T01:13:34Z-
dc.date.available2014-07-22T01:13:34Z-
dc.date.issued2014en_US
dc.identifier.citationThe 92nd General Session & Exhibition of the International Association for Dental Research (IADR), Cape Town, South Africa, 25-28 June 2014. In Journal of Dental Research, 2014, v. 93 n. Special issue B: abstract no. 1394en_US
dc.identifier.issn0022-0345-
dc.identifier.urihttp://hdl.handle.net/10722/199315-
dc.descriptionOral Presentation-
dc.descriptionSession 193: Keynote Address; Adhesive Interaction with Enamel, Dentin and New Materials-
dc.description.abstractObjective: To investigate the ultra-structural changes produced by phosphoric acid (PA) and self-etching primer (SEP) on intact enamel surface. Methods: Sound human teeth were collected with ethical approval (IRB No.: UW13-262) and randomly assigned to four groups: Group A (40% PA liquid), Group B (40% PA gel), Group C (Clearfil SE Bond primer, Kuraray Co.), and Group D (Transbond Plus SEP, 3M Unitek). Four coronal central buccal enamel specimens with surface dimension of 1x1.5 mm2 were sectioned and gently cleansed. In groups A and B, specimens were etched for 0, 15, 30, and 60 seconds, respectively, rinsed thoroughly with de-ionized water and dried afterwards. In groups C and D, SEPs were applied following manufacturer’s instructions. Specimens without treatment were set as control. Surface chemical composition, crystalline structure, and morphology were determined by energy dispersive x-ray spectroscopy (EDX), x-ray diffraction (XRD) and scanning electron microscopy (SEM). Results: Calcium to phosphate (Ca/P) ratio was calculated and analyzed by two-way analysis of variance (α=0.05). After PA-etch, Ca/P ratio was initially increased and then decreased, without significant differences among various etching durations (p>0.05). Statistical difference was demonstrated between liquid and gel (p=0.016). Application of SEP dramatically decreased the Ca/P ratio (p<0.001), which was also significantly influenced by the type of SEP (p=0.003). XRD spectra revealed existence of new phosphate compounds in all groups, while intensity changes of hydroxyapatite characteristic peaks were only found in groups A and B. SEM exhibited typical etch patterns generated by PA-etch. Whereas, very conservative etch pattern was produced by SEP, which masked enamel surface with a hybrid layer. Conclusion: PA-etch dissolved the superficial layer of intact enamel, changed the crystalline structure of enamel hydroxyapatite and efficiently roughed the surface. SEP had limited dissolution ability and less actively reacted with enamel substrate. However, new phosphate compounds were formed by either technique.-
dc.languageengen_US
dc.publisherSage Publications, Inc.. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925-
dc.relation.ispartofJournal of Dental Researchen_US
dc.rightsJournal of Dental Research. Copyright © Sage Publications, Inc..-
dc.subjectAcid etch-
dc.subjectDental materials-
dc.subjectEnamel-
dc.subjectHistology - ultrastructure and Self-etching primer-
dc.titleEtch-and-rinse versus Self-etch Pretreatment: the Ultra-structural Investigation on Intact Enamelen_US
dc.typeConference_Paperen_US
dc.identifier.emailTsoi, KH: jkhtsoi@hku.hken_US
dc.identifier.emailTang, ATH: athtang@hku.hken_US
dc.identifier.emailHagg, EUO: euohagg@hku.hken_US
dc.identifier.emailMatinlinna, JP: jpmat@hku.hken_US
dc.identifier.authorityTsoi, KH=rp01609en_US
dc.identifier.authorityTang, ATH=rp00054en_US
dc.identifier.authorityHagg, EUO=rp00020en_US
dc.identifier.authorityMatinlinna, JP=rp00052en_US
dc.identifier.hkuros230566en_US
dc.identifier.volume93-
dc.identifier.issueSpecial issue B: abstract no. 1394-
dc.publisher.placeUnited States-

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