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Conference Paper: Reduced level of induced endocytosis in Candida albicans sap7 mutants

TitleReduced level of induced endocytosis in Candida albicans sap7 mutants
Authors
KeywordsGenetics
Host-microbial interactions
Infection
Pathogenicity
Issue Date2014
PublisherInternational Association for Dental Research. The Conference abstracts' website is located at https://iadr.confex.com/iadr/search.epl
Citation
The 2014 International Association for Dental Research (IADR) - Pan European Regional (PER) Congress, Dubrovnik, Croatia, 10-13 September 2014. How to Cite?
AbstractOBJECTIVE: To investigate the effect of C. albicans SAP7 gene in induced endocytosis in Candida-epithelial cell interactions. METHOD: In this study, C. albicans sap7 mutant strain was constructed in our laboratory by a PCR – based gene disruption method. In the endocytosis assay, the number of C. albicans cells that were cell-associated with and endocytosed by OKF6/TERT2 was determined using differential fluorescence assay with minor modifications. Briefly, conflucent OKF6/TERT2 cells were infected with 1×105 Candida cells for 3 h incubation. After removing nonadherent organisms, the cells were fixed with 3% paraformaldehyde. The adherent but not endocytosed ...
DescriptionPoster Session: Scientific Groups - 90. Microbiology/Immunology III: no. 655
Persistent Identifierhttp://hdl.handle.net/10722/198156

 

DC FieldValueLanguage
dc.contributor.authorYang, H-
dc.contributor.authorTsang, PCS-
dc.contributor.authorPow, EHN-
dc.contributor.authorTsang, PWK-
dc.date.accessioned2014-06-25T02:49:36Z-
dc.date.available2014-06-25T02:49:36Z-
dc.date.issued2014-
dc.identifier.citationThe 2014 International Association for Dental Research (IADR) - Pan European Regional (PER) Congress, Dubrovnik, Croatia, 10-13 September 2014.-
dc.identifier.urihttp://hdl.handle.net/10722/198156-
dc.descriptionPoster Session: Scientific Groups - 90. Microbiology/Immunology III: no. 655-
dc.description.abstractOBJECTIVE: To investigate the effect of C. albicans SAP7 gene in induced endocytosis in Candida-epithelial cell interactions. METHOD: In this study, C. albicans sap7 mutant strain was constructed in our laboratory by a PCR – based gene disruption method. In the endocytosis assay, the number of C. albicans cells that were cell-associated with and endocytosed by OKF6/TERT2 was determined using differential fluorescence assay with minor modifications. Briefly, conflucent OKF6/TERT2 cells were infected with 1×105 Candida cells for 3 h incubation. After removing nonadherent organisms, the cells were fixed with 3% paraformaldehyde. The adherent but not endocytosed ...-
dc.languageeng-
dc.publisherInternational Association for Dental Research. The Conference abstracts' website is located at https://iadr.confex.com/iadr/search.epl-
dc.relation.ispartofIADR/PER Congress 2014-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectGenetics-
dc.subjectHost-microbial interactions-
dc.subjectInfection-
dc.subjectPathogenicity-
dc.titleReduced level of induced endocytosis in Candida albicans sap7 mutants-
dc.typeConference_Paper-
dc.identifier.emailTsang, PCS: csptsang@hkucc.hku.hk-
dc.identifier.emailPow, EHN: ehnpow@hku.hk-
dc.identifier.emailTsang, PWK: pwktsang@hku.hk-
dc.identifier.authorityTsang, PCS=rp00026-
dc.identifier.authorityPow, EHN=rp00030-
dc.identifier.authorityTsang, PWK=rp01388-
dc.description.naturepostprint-
dc.identifier.hkuros229297-
dc.publisher.placeUnited States-

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