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Article: Long non-coding RNA deregulation in tongue squamous cell carcinoma

TitleLong non-coding RNA deregulation in tongue squamous cell carcinoma
Authors
Issue Date2014
PublisherHindawi Publishing Corporation. The Journal's web site is located at http://www.hindawi.com/journals/jbb/index.html
Citation
Biomed Research International , 2014, v. 2014, article no. 405860 How to Cite?
AbstractBackground. The deregulated tumorigenic long non-coding RNA (lncRNA) has been reported in several malignancies. However, there is still no comprehensive study on tongue squamous cell carcinoma (SCC). Methods. Functional reannotation for the human lncRNA was carried out by ncFANs. Real-time quantitative PCR was used to validate the identified lncRNAs. Results. Using the functional annotation algorithm from ncFANs, 8 differentially expressed lncRNAs were identified. Lnc-PPP2R4-5, lnc-SPRR2D-1, lnc-MAN1A2-1, lnc-FAM46A-1, lnc-MBL2-4:1, and lnc-MBL2-4:3 were upregulated in the microdissected tongue SCC tissues. In comparison, lnc-AL355149.1-1 and lnc-STXBP5-1 showed significant downregulation. High level of lnc-MBL2-4:3 was significantly associated with the node positive tongue SCC patients. Further, patients with advanced T-stage demonstrated a further reduction of lnc-AL355149.1-1 in the tumor tissues. Treatment of tongue SCC cells with 5-fluorouracil and paclitaxel can reserve the expression patterns observed in the tongue SCC tissues. Further, changes of lnc-MBL2-4:3 and lnc-AL355149.1-1 expression levels were noticed in the cisplatin-resistant tongue SCC cells. Conclusions. Our results demonstrated that functional reannotation allows us to identify novel lncRNAs using the existing gene expression array dataset. The association of lncRNA with the T-stage and nodal status of tongue SCC patients suggested that lncRNA deregulation was involved in the pathogenesis of tongue SCC.
Persistent Identifierhttp://hdl.handle.net/10722/198111
ISSN
2015 Impact Factor: 2.134
2015 SCImago Journal Rankings: 0.725
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorGao, Wen_US
dc.contributor.authorChan, JYWen_US
dc.contributor.authorWong, TSen_US
dc.date.accessioned2014-06-25T02:47:31Z-
dc.date.available2014-06-25T02:47:31Z-
dc.date.issued2014en_US
dc.identifier.citationBiomed Research International , 2014, v. 2014, article no. 405860en_US
dc.identifier.issn2314-6133en_US
dc.identifier.urihttp://hdl.handle.net/10722/198111-
dc.description.abstractBackground. The deregulated tumorigenic long non-coding RNA (lncRNA) has been reported in several malignancies. However, there is still no comprehensive study on tongue squamous cell carcinoma (SCC). Methods. Functional reannotation for the human lncRNA was carried out by ncFANs. Real-time quantitative PCR was used to validate the identified lncRNAs. Results. Using the functional annotation algorithm from ncFANs, 8 differentially expressed lncRNAs were identified. Lnc-PPP2R4-5, lnc-SPRR2D-1, lnc-MAN1A2-1, lnc-FAM46A-1, lnc-MBL2-4:1, and lnc-MBL2-4:3 were upregulated in the microdissected tongue SCC tissues. In comparison, lnc-AL355149.1-1 and lnc-STXBP5-1 showed significant downregulation. High level of lnc-MBL2-4:3 was significantly associated with the node positive tongue SCC patients. Further, patients with advanced T-stage demonstrated a further reduction of lnc-AL355149.1-1 in the tumor tissues. Treatment of tongue SCC cells with 5-fluorouracil and paclitaxel can reserve the expression patterns observed in the tongue SCC tissues. Further, changes of lnc-MBL2-4:3 and lnc-AL355149.1-1 expression levels were noticed in the cisplatin-resistant tongue SCC cells. Conclusions. Our results demonstrated that functional reannotation allows us to identify novel lncRNAs using the existing gene expression array dataset. The association of lncRNA with the T-stage and nodal status of tongue SCC patients suggested that lncRNA deregulation was involved in the pathogenesis of tongue SCC.-
dc.languageengen_US
dc.publisherHindawi Publishing Corporation. The Journal's web site is located at http://www.hindawi.com/journals/jbb/index.htmlen_US
dc.relation.ispartofBiomed Research Internationalen_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titleLong non-coding RNA deregulation in tongue squamous cell carcinomaen_US
dc.typeArticleen_US
dc.identifier.emailGao, W: weigao@graduate.hku.hken_US
dc.identifier.emailChan, JYW: jywchan1@hku.hken_US
dc.identifier.emailWong, TS: thiansze@graduate.hku.hken_US
dc.identifier.authorityChan, JYW=rp01314en_US
dc.identifier.authorityWong, TS=rp00478en_US
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1155/2014/405860-
dc.identifier.pmid25045670-
dc.identifier.pmcidPMC4090519-
dc.identifier.scopuseid_2-s2.0-84904136232-
dc.identifier.hkuros229329en_US
dc.identifier.hkuros229895-
dc.identifier.volume2014-
dc.identifier.isiWOS:000338558300001-
dc.publisher.placeUnited Statesen_US

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