File Download
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1155/2014/405860
- Scopus: eid_2-s2.0-84904136232
- PMID: 25045670
- WOS: WOS:000338558300001
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Long non-coding RNA deregulation in tongue squamous cell carcinoma
Title | Long non-coding RNA deregulation in tongue squamous cell carcinoma |
---|---|
Authors | |
Issue Date | 2014 |
Publisher | Hindawi Publishing Corporation. The Journal's web site is located at http://www.hindawi.com/journals/jbb/index.html |
Citation | Biomed Research International , 2014, v. 2014, article no. 405860 How to Cite? |
Abstract | Background. The deregulated tumorigenic long non-coding RNA (lncRNA) has been reported in several malignancies. However, there is still no comprehensive study on tongue squamous cell carcinoma (SCC). Methods. Functional reannotation for the human lncRNA was carried out by ncFANs. Real-time quantitative PCR was used to validate the identified lncRNAs. Results. Using the functional annotation algorithm from ncFANs, 8 differentially expressed lncRNAs were identified. Lnc-PPP2R4-5, lnc-SPRR2D-1, lnc-MAN1A2-1, lnc-FAM46A-1, lnc-MBL2-4:1, and lnc-MBL2-4:3 were upregulated in the microdissected tongue SCC tissues. In comparison, lnc-AL355149.1-1 and lnc-STXBP5-1 showed significant downregulation. High level of lnc-MBL2-4:3 was significantly associated with the node positive tongue SCC patients. Further, patients with advanced T-stage demonstrated a further reduction of lnc-AL355149.1-1 in the tumor tissues. Treatment of tongue SCC cells with 5-fluorouracil and paclitaxel can reserve the expression patterns observed in the tongue SCC tissues. Further, changes of lnc-MBL2-4:3 and lnc-AL355149.1-1 expression levels were noticed in the cisplatin-resistant tongue SCC cells. Conclusions. Our results demonstrated that functional reannotation allows us to identify novel lncRNAs using the existing gene expression array dataset. The association of lncRNA with the T-stage and nodal status of tongue SCC patients suggested that lncRNA deregulation was involved in the pathogenesis of tongue SCC. |
Persistent Identifier | http://hdl.handle.net/10722/198111 |
ISSN | 2023 Impact Factor: 2.6 2023 SCImago Journal Rankings: 0.656 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Gao, W | en_US |
dc.contributor.author | Chan, JYW | en_US |
dc.contributor.author | Wong, TS | en_US |
dc.date.accessioned | 2014-06-25T02:47:31Z | - |
dc.date.available | 2014-06-25T02:47:31Z | - |
dc.date.issued | 2014 | en_US |
dc.identifier.citation | Biomed Research International , 2014, v. 2014, article no. 405860 | en_US |
dc.identifier.issn | 2314-6133 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/198111 | - |
dc.description.abstract | Background. The deregulated tumorigenic long non-coding RNA (lncRNA) has been reported in several malignancies. However, there is still no comprehensive study on tongue squamous cell carcinoma (SCC). Methods. Functional reannotation for the human lncRNA was carried out by ncFANs. Real-time quantitative PCR was used to validate the identified lncRNAs. Results. Using the functional annotation algorithm from ncFANs, 8 differentially expressed lncRNAs were identified. Lnc-PPP2R4-5, lnc-SPRR2D-1, lnc-MAN1A2-1, lnc-FAM46A-1, lnc-MBL2-4:1, and lnc-MBL2-4:3 were upregulated in the microdissected tongue SCC tissues. In comparison, lnc-AL355149.1-1 and lnc-STXBP5-1 showed significant downregulation. High level of lnc-MBL2-4:3 was significantly associated with the node positive tongue SCC patients. Further, patients with advanced T-stage demonstrated a further reduction of lnc-AL355149.1-1 in the tumor tissues. Treatment of tongue SCC cells with 5-fluorouracil and paclitaxel can reserve the expression patterns observed in the tongue SCC tissues. Further, changes of lnc-MBL2-4:3 and lnc-AL355149.1-1 expression levels were noticed in the cisplatin-resistant tongue SCC cells. Conclusions. Our results demonstrated that functional reannotation allows us to identify novel lncRNAs using the existing gene expression array dataset. The association of lncRNA with the T-stage and nodal status of tongue SCC patients suggested that lncRNA deregulation was involved in the pathogenesis of tongue SCC. | - |
dc.language | eng | en_US |
dc.publisher | Hindawi Publishing Corporation. The Journal's web site is located at http://www.hindawi.com/journals/jbb/index.html | en_US |
dc.relation.ispartof | Biomed Research International | en_US |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.title | Long non-coding RNA deregulation in tongue squamous cell carcinoma | en_US |
dc.type | Article | en_US |
dc.identifier.email | Gao, W: weigao@graduate.hku.hk | en_US |
dc.identifier.email | Chan, JYW: jywchan1@hku.hk | en_US |
dc.identifier.email | Wong, TS: thiansze@graduate.hku.hk | en_US |
dc.identifier.authority | Chan, JYW=rp01314 | en_US |
dc.identifier.authority | Wong, TS=rp00478 | en_US |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.1155/2014/405860 | - |
dc.identifier.pmid | 25045670 | - |
dc.identifier.pmcid | PMC4090519 | - |
dc.identifier.scopus | eid_2-s2.0-84904136232 | - |
dc.identifier.hkuros | 229329 | en_US |
dc.identifier.hkuros | 229895 | - |
dc.identifier.volume | 2014 | - |
dc.identifier.isi | WOS:000338558300001 | - |
dc.publisher.place | United States | en_US |
dc.identifier.issnl | 2314-6133 | - |