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Article: Vaults are dynamically unconstrained cytoplasmic nanoparticles capable of half vault exchange

TitleVaults are dynamically unconstrained cytoplasmic nanoparticles capable of half vault exchange
Authors
Issue Date2010
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journals/ancac3/index.html
Citation
ACS Nano, 2010, v. 4 n. 12, p. 7229-7240 How to Cite?
AbstractVaults are naturally occurring ribonucleoprotein particles with an enormous interior volume, large enough to encapsulate hundreds of proteins. They are highly conserved and are present in nearly all eukaryotic cells ranging from 10(4) to 10(7) particles per cell. Recombinant vaults can be produced in vitro and engineered to allow cell targeting and protein packaging. These nanometer-sized particles have many desirable characteristics that may give them advantages for use as drug delivery vehicles. Using photoactivatable green fluorescent protein (PAGFP) labeled vaults, we demonstrate that the particles rapidly diffuse throughout the cytoplasm following single pixel photoactivation in live cells. Their in vivo movement remained relatively unchanged despite exposure to a variety of cellular stresses, suggesting that vaults are largely unconstrained in the cytoplasm. Fluorescence resonance energy transfer (FRET) was observed from polyethylene glycol (PEG) fused hybrid cells that expressed either CFP or YFP labeled vaults, indicating that vaults can exchange major vault protein (MVP) subunits in vivo. Investigation into the mechanism of this exchange in vitro using recombinant vaults demonstrated that they were capable of rapidly separating at the particle waist and reassembling back into whole vaults, supporting a half vault exchange mechanism. This data suggests a means whereby vaults can functionally interact with their cellular environment and deliver materials packaged within their interior.
Persistent Identifierhttp://hdl.handle.net/10722/197919
ISSN
2015 Impact Factor: 13.334
2015 SCImago Journal Rankings: 7.120
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorYang, J-
dc.contributor.authorKickhoefer, VA-
dc.contributor.authorNg, BC-
dc.contributor.authorGopal, A-
dc.contributor.authorBentolila, LA-
dc.contributor.authorJohn, S-
dc.contributor.authorTolbert, SH-
dc.contributor.authorRome, LH-
dc.date.accessioned2014-06-11T07:13:44Z-
dc.date.available2014-06-11T07:13:44Z-
dc.date.issued2010-
dc.identifier.citationACS Nano, 2010, v. 4 n. 12, p. 7229-7240-
dc.identifier.issn1936-0851-
dc.identifier.urihttp://hdl.handle.net/10722/197919-
dc.description.abstractVaults are naturally occurring ribonucleoprotein particles with an enormous interior volume, large enough to encapsulate hundreds of proteins. They are highly conserved and are present in nearly all eukaryotic cells ranging from 10(4) to 10(7) particles per cell. Recombinant vaults can be produced in vitro and engineered to allow cell targeting and protein packaging. These nanometer-sized particles have many desirable characteristics that may give them advantages for use as drug delivery vehicles. Using photoactivatable green fluorescent protein (PAGFP) labeled vaults, we demonstrate that the particles rapidly diffuse throughout the cytoplasm following single pixel photoactivation in live cells. Their in vivo movement remained relatively unchanged despite exposure to a variety of cellular stresses, suggesting that vaults are largely unconstrained in the cytoplasm. Fluorescence resonance energy transfer (FRET) was observed from polyethylene glycol (PEG) fused hybrid cells that expressed either CFP or YFP labeled vaults, indicating that vaults can exchange major vault protein (MVP) subunits in vivo. Investigation into the mechanism of this exchange in vitro using recombinant vaults demonstrated that they were capable of rapidly separating at the particle waist and reassembling back into whole vaults, supporting a half vault exchange mechanism. This data suggests a means whereby vaults can functionally interact with their cellular environment and deliver materials packaged within their interior.-
dc.languageeng-
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journals/ancac3/index.html-
dc.relation.ispartofACS Nano-
dc.subject.meshCytoplasm - metabolism-
dc.subject.meshFluorescence Resonance Energy Transfer-
dc.subject.meshNanoparticles-
dc.subject.meshPolyethylene Glycols - chemistry-
dc.subject.meshVault Ribonucleoprotein Particles - chemistry - metabolism-
dc.titleVaults are dynamically unconstrained cytoplasmic nanoparticles capable of half vault exchangeen_US
dc.typeArticleen_US
dc.identifier.emailYang, J: jianyang@hku.hk-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1021/nn102051r-
dc.identifier.pmid21121616-
dc.identifier.pmcidPMC3020078-
dc.identifier.hkuros202526-
dc.identifier.volume4-
dc.identifier.issue12-
dc.identifier.spage7229-
dc.identifier.epage7240-
dc.identifier.isiWOS:000285449100024-
dc.publisher.placeUnited States-

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